Prostaglandin A1 inhibits the replication of Sindbis virus in monkey kidney and mosquito cells

Detalhes bibliográficos
Autor(a) principal: Gutarra, Melissa L. E.
Data de Publicação: 2008
Outros Autores: Burlandy, Fernanda Marcicano, Meneses, Marcelo Damião Ferreira de, Cortez, Letícia P., Rebello, Moacyr A.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/20540
Resumo: Abstracts:The present study reports the effect of prostaglandin A1 (PGA1) on the replication of Sindbis virus in monkey kidney and mosquito cells. In PGA1 treated cells we observed a severe reduction of virus yield. In both cells lines the highest nontoxic concentration of PGA1 (10 ìg/mL) decreased virus replication, dose dependently, by more than 90%. SDS-PAGE analysis of [35S] methionine labeled proteins showed that viral proteins (E1/E2 and C) were normally synthesized in PGA1 treated Vero cells, and induction of stress proteins (HSP70 and HSP90 ) was detected in uninfected and infected cells. In Vero cells the inhibition of virus replication was accompanied by a decrease in [3H] glucosamine incorporation into the virus glycoproteins.
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spelling Gutarra, Melissa L. E.Burlandy, Fernanda MarcicanoMeneses, Marcelo Damião Ferreira deCortez, Letícia P.Rebello, Moacyr A.Gutarra, Melissa L. E.Burlandy, Fernanda MarcicanoMeneses, Marcelo Damião Ferreira deCortez, Letícia P.Rebello, Moacyr A.2016-09-15T16:36:32Z2016-09-15T16:36:32Z2008-05GUTARRA, M. L. E. et al. Prostaglandin A1 inhibits the replication of Sindbis virus in monkey kidney and mosquito cells. R. Ci. méd. biol., Salvador, v. 7, n.2, p.169-174, mai./ago. 2008.2236-5222http://repositorio.ufba.br/ri/handle/ri/20540Abstracts:The present study reports the effect of prostaglandin A1 (PGA1) on the replication of Sindbis virus in monkey kidney and mosquito cells. In PGA1 treated cells we observed a severe reduction of virus yield. In both cells lines the highest nontoxic concentration of PGA1 (10 ìg/mL) decreased virus replication, dose dependently, by more than 90%. SDS-PAGE analysis of [35S] methionine labeled proteins showed that viral proteins (E1/E2 and C) were normally synthesized in PGA1 treated Vero cells, and induction of stress proteins (HSP70 and HSP90 ) was detected in uninfected and infected cells. In Vero cells the inhibition of virus replication was accompanied by a decrease in [3H] glucosamine incorporation into the virus glycoproteins.Neste estudo nós avaliamos o efeito da prostaglandina A1 (PGA1) na replicação do vírus Síndbis em células de macaco e em células de mosquito. Nas células tratadas com PGA1 nós observamos uma redução significativa na produção de partículas virais infecciosas. Em ambas as linhagens celulares tratadas com concentrações não-tóxicas de PGA1 (1-10 μg/ml) foi observado uma redução da replicação viral, de forma dose dependente, chegando a 90% na maior dose utilizada (10 μg/ml). A análise das proteínas virais e celulares marcadas com [35S]-metionina em SDS-PAGE mostrou que as proteínas virais (E1, E2 e C) foram normalmente sintetizas em células Vero tratadas com PGA1. Além disso, observamos também a indução das proteínas de estresse (HSP70 e HSP90) nas células Vero infectadas ou não infectadas. Em células Vero foi observado uma diminuição na incorporação de [3H] glicosamina nas glicoproteínas virais.Submitted by ROBERTO PAULO CORREIA DE ARAÚJO (ppgorgsistem@ufba.br) on 2016-09-15T16:36:32Z No. of bitstreams: 1 8_v.7_2.pdf: 88426 bytes, checksum: aa8e6738bdc1a420ba44ebd057cca03b (MD5)Made available in DSpace on 2016-09-15T16:36:32Z (GMT). No. of bitstreams: 1 8_v.7_2.pdf: 88426 bytes, checksum: aa8e6738bdc1a420ba44ebd057cca03b (MD5) Previous issue date: 2008-05SalvadorInstituto de Ciências da Saúde/ Universidade Federal da BahiaBrasilhttp://www.portalseer.ufba.br/index.php/cmbio/article/view/4447/3300reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAProstaglandina A1.Vírus Síndbis.Células de Aedes albopictus.Vírus Síndbis- PGA.Prostaglandin A1 inhibits the replication of Sindbis virus in monkey kidney and mosquito cellsRevista de Ciências Médicas e Biológicasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessporORIGINAL8_v.7_2.pdf8_v.7_2.pdfapplication/pdf88426https://repositorio.ufba.br/bitstream/ri/20540/1/8_v.7_2.pdfaa8e6738bdc1a420ba44ebd057cca03bMD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/20540/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXT8_v.7_2.pdf.txt8_v.7_2.pdf.txtExtracted texttext/plain20415https://repositorio.ufba.br/bitstream/ri/20540/3/8_v.7_2.pdf.txt4395246fa22c28bd13ccf73fdf27fa5eMD53ri/205402022-07-05 14:03:52.198oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:52Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Prostaglandin A1 inhibits the replication of Sindbis virus in monkey kidney and mosquito cells
dc.title.alternative.pt_BR.fl_str_mv Revista de Ciências Médicas e Biológicas
title Prostaglandin A1 inhibits the replication of Sindbis virus in monkey kidney and mosquito cells
spellingShingle Prostaglandin A1 inhibits the replication of Sindbis virus in monkey kidney and mosquito cells
Gutarra, Melissa L. E.
Prostaglandina A1.
Vírus Síndbis.
Células de Aedes albopictus.
Vírus Síndbis- PGA.
title_short Prostaglandin A1 inhibits the replication of Sindbis virus in monkey kidney and mosquito cells
title_full Prostaglandin A1 inhibits the replication of Sindbis virus in monkey kidney and mosquito cells
title_fullStr Prostaglandin A1 inhibits the replication of Sindbis virus in monkey kidney and mosquito cells
title_full_unstemmed Prostaglandin A1 inhibits the replication of Sindbis virus in monkey kidney and mosquito cells
title_sort Prostaglandin A1 inhibits the replication of Sindbis virus in monkey kidney and mosquito cells
author Gutarra, Melissa L. E.
author_facet Gutarra, Melissa L. E.
Burlandy, Fernanda Marcicano
Meneses, Marcelo Damião Ferreira de
Cortez, Letícia P.
Rebello, Moacyr A.
author_role author
author2 Burlandy, Fernanda Marcicano
Meneses, Marcelo Damião Ferreira de
Cortez, Letícia P.
Rebello, Moacyr A.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Gutarra, Melissa L. E.
Burlandy, Fernanda Marcicano
Meneses, Marcelo Damião Ferreira de
Cortez, Letícia P.
Rebello, Moacyr A.
Gutarra, Melissa L. E.
Burlandy, Fernanda Marcicano
Meneses, Marcelo Damião Ferreira de
Cortez, Letícia P.
Rebello, Moacyr A.
dc.subject.por.fl_str_mv Prostaglandina A1.
Vírus Síndbis.
Células de Aedes albopictus.
Vírus Síndbis- PGA.
topic Prostaglandina A1.
Vírus Síndbis.
Células de Aedes albopictus.
Vírus Síndbis- PGA.
description Abstracts:The present study reports the effect of prostaglandin A1 (PGA1) on the replication of Sindbis virus in monkey kidney and mosquito cells. In PGA1 treated cells we observed a severe reduction of virus yield. In both cells lines the highest nontoxic concentration of PGA1 (10 ìg/mL) decreased virus replication, dose dependently, by more than 90%. SDS-PAGE analysis of [35S] methionine labeled proteins showed that viral proteins (E1/E2 and C) were normally synthesized in PGA1 treated Vero cells, and induction of stress proteins (HSP70 and HSP90 ) was detected in uninfected and infected cells. In Vero cells the inhibition of virus replication was accompanied by a decrease in [3H] glucosamine incorporation into the virus glycoproteins.
publishDate 2008
dc.date.issued.fl_str_mv 2008-05
dc.date.accessioned.fl_str_mv 2016-09-15T16:36:32Z
dc.date.available.fl_str_mv 2016-09-15T16:36:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GUTARRA, M. L. E. et al. Prostaglandin A1 inhibits the replication of Sindbis virus in monkey kidney and mosquito cells. R. Ci. méd. biol., Salvador, v. 7, n.2, p.169-174, mai./ago. 2008.
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/20540
dc.identifier.issn.none.fl_str_mv 2236-5222
identifier_str_mv GUTARRA, M. L. E. et al. Prostaglandin A1 inhibits the replication of Sindbis virus in monkey kidney and mosquito cells. R. Ci. méd. biol., Salvador, v. 7, n.2, p.169-174, mai./ago. 2008.
2236-5222
url http://repositorio.ufba.br/ri/handle/ri/20540
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Instituto de Ciências da Saúde/ Universidade Federal da Bahia
dc.publisher.country.fl_str_mv Brasil
publisher.none.fl_str_mv Instituto de Ciências da Saúde/ Universidade Federal da Bahia
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