Experimental Infection of Rhodnius prolixus (Hemiptera, Triatominae) with Mycobacterium leprae Indicates Potential for Leprosy Transmission
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/14769 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. |
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Neumann, Arthur da SilvaDias, Felipe de AlmeidaFerreira, Jéssica da SilvaFontes, Amanda Nogueira BrumRosa, Patricia SammarcoMacedo, Rafael EnriqueOliveira, José HenriqueTeixeira, Raquel Lima de FigueiredoPessolani, Maria Cristina VidalMoraes, Milton OzórioSuffys, Philip NoelOliveira, Pedro L.Sorgine, Marcos Henrique FerreiraLara, Flavio Alves2016-07-05T13:20:09Z2016-07-08T12:21:57Z2016-07-05T13:20:09Z2016-07-08T12:21:57Z2016NEUMANN, Arthur da Silva; et al. Experimental Infection of Rhodnius prolixus (Hemiptera, Triatominae) with Mycobacterium leprae Indicates Potential for Leprosy Transmission. Plos One, v.11, n.5, e0156037, 14p, May 2016.1932-6203https://www.arca.fiocruz.br/handle/icict/1476910.1371/journal.pone.0156037engPublic Library of ScienceExperimental Infection of Rhodnius prolixus (Hemiptera, Triatominae) with Mycobacterium leprae Indicates Potential for Leprosy Transmissioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Laboratório de Bioquímica de Artrópodes Hematófagos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Instituto Lauro de Sousa Lima. Departmento de Biologia, Bauru, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Laboratório de Bioquímica de Artrópodes Hematófagos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Laboratório de Bioquímica de Artrópodes Hematófagos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Laboratório de Bioquímica de Artrópodes Hematófagos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Leprosy is a chronic dermato-neurological disease caused by infection with Mycobacterium leprae. In 2013 almost 200,000 new cases of leprosy were detected around the world. Since the first symptoms take from years to decades to appear, the total number of asymptomatic patients is impossible to predict. Although leprosy is one of the oldest records of human disease, the mechanisms involved with its transmission and epidemiology are still not completely understood. In the present work, we experimentally investigated the hypothesis that the mosquitoes Aedes aegypti and Culex quinquefasciatus and the hemiptera Rhodnius prolixus act as leprosy vectors. By means of real-time PCR quantification of M. leprae 16SrRNA, we found that M. leprae remained viable inside the digestive tract of Rhodnius prolixus for 20 days after oral infection. In contrast, in the gut of both mosquito species tested, we were not able to detect M. leprae RNA after a similar period of time. Inside the kissing bug Rhodnius prolixus digestive tract, M. leprae was initially restricted to the anterior midgut, but gradually moved towards the hindgut, in a time course reminiscent of the life cycle of Trypanosoma cruzi, a well-known pathogen transmitted by this insect. The maintenance of M. leprae infectivity inside the digestive tract of this kissing bug is further supported by successful mice footpad inoculation with feces collected 20 days after infection.We conclude that Rhodnius prolixus defecate infective M. leprae, justifying the evaluation of the presence of M. leprae among sylvatic and domestic kissing bugs in countries endemic for leprosy.LeprosyMycobacterium lepraeRhodnius prolixusInfectionHanseníaseMycobacterium lepraeHemípterosTriatominaeInfecçãoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTarthur_neumann_etal_IOC_2016.pdf.txtarthur_neumann_etal_IOC_2016.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Experimental Infection of Rhodnius prolixus (Hemiptera, Triatominae) with Mycobacterium leprae Indicates Potential for Leprosy Transmission |
| title |
Experimental Infection of Rhodnius prolixus (Hemiptera, Triatominae) with Mycobacterium leprae Indicates Potential for Leprosy Transmission |
| spellingShingle |
Experimental Infection of Rhodnius prolixus (Hemiptera, Triatominae) with Mycobacterium leprae Indicates Potential for Leprosy Transmission Neumann, Arthur da Silva Leprosy Mycobacterium leprae Rhodnius prolixus Infection Hanseníase Mycobacterium leprae Hemípteros Triatominae Infecção |
| title_short |
Experimental Infection of Rhodnius prolixus (Hemiptera, Triatominae) with Mycobacterium leprae Indicates Potential for Leprosy Transmission |
| title_full |
Experimental Infection of Rhodnius prolixus (Hemiptera, Triatominae) with Mycobacterium leprae Indicates Potential for Leprosy Transmission |
| title_fullStr |
Experimental Infection of Rhodnius prolixus (Hemiptera, Triatominae) with Mycobacterium leprae Indicates Potential for Leprosy Transmission |
| title_full_unstemmed |
Experimental Infection of Rhodnius prolixus (Hemiptera, Triatominae) with Mycobacterium leprae Indicates Potential for Leprosy Transmission |
| title_sort |
Experimental Infection of Rhodnius prolixus (Hemiptera, Triatominae) with Mycobacterium leprae Indicates Potential for Leprosy Transmission |
| author |
Neumann, Arthur da Silva |
| author_facet |
Neumann, Arthur da Silva Dias, Felipe de Almeida Ferreira, Jéssica da Silva Fontes, Amanda Nogueira Brum Rosa, Patricia Sammarco Macedo, Rafael Enrique Oliveira, José Henrique Teixeira, Raquel Lima de Figueiredo Pessolani, Maria Cristina Vidal Moraes, Milton Ozório Suffys, Philip Noel Oliveira, Pedro L. Sorgine, Marcos Henrique Ferreira Lara, Flavio Alves |
| author_role |
author |
| author2 |
Dias, Felipe de Almeida Ferreira, Jéssica da Silva Fontes, Amanda Nogueira Brum Rosa, Patricia Sammarco Macedo, Rafael Enrique Oliveira, José Henrique Teixeira, Raquel Lima de Figueiredo Pessolani, Maria Cristina Vidal Moraes, Milton Ozório Suffys, Philip Noel Oliveira, Pedro L. Sorgine, Marcos Henrique Ferreira Lara, Flavio Alves |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Neumann, Arthur da Silva Dias, Felipe de Almeida Ferreira, Jéssica da Silva Fontes, Amanda Nogueira Brum Rosa, Patricia Sammarco Macedo, Rafael Enrique Oliveira, José Henrique Teixeira, Raquel Lima de Figueiredo Pessolani, Maria Cristina Vidal Moraes, Milton Ozório Suffys, Philip Noel Oliveira, Pedro L. Sorgine, Marcos Henrique Ferreira Lara, Flavio Alves |
| dc.subject.en.pt_BR.fl_str_mv |
Leprosy Mycobacterium leprae Rhodnius prolixus Infection |
| topic |
Leprosy Mycobacterium leprae Rhodnius prolixus Infection Hanseníase Mycobacterium leprae Hemípteros Triatominae Infecção |
| dc.subject.decs.pt_BR.fl_str_mv |
Hanseníase Mycobacterium leprae Hemípteros Triatominae Infecção |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. |
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2016 |
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2016-07-05T13:20:09Z 2016-07-08T12:21:57Z |
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2016-07-05T13:20:09Z 2016-07-08T12:21:57Z |
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2016 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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NEUMANN, Arthur da Silva; et al. Experimental Infection of Rhodnius prolixus (Hemiptera, Triatominae) with Mycobacterium leprae Indicates Potential for Leprosy Transmission. Plos One, v.11, n.5, e0156037, 14p, May 2016. |
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https://www.arca.fiocruz.br/handle/icict/14769 |
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1932-6203 |
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10.1371/journal.pone.0156037 |
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NEUMANN, Arthur da Silva; et al. Experimental Infection of Rhodnius prolixus (Hemiptera, Triatominae) with Mycobacterium leprae Indicates Potential for Leprosy Transmission. Plos One, v.11, n.5, e0156037, 14p, May 2016. 1932-6203 10.1371/journal.pone.0156037 |
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