Inhibition of digestive enzymes (α-amylase, αglucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract

Detalhes bibliográficos
Autor(a) principal: Carvalho, Caio Eduardo de
Data de Publicação: 2022
Outros Autores: Mancini, Daiana Teixeira, Pereira, Chrystian Araújo, Thomasi, Sérgio Scherrer
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/55251
Resumo: Universidade Federal de Lavras. Faculdade de Ciências da Saúde. Lavras, MG, Brasil.
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spelling Carvalho, Caio Eduardo deMancini, Daiana TeixeiraPereira, Chrystian AraújoThomasi, Sérgio Scherrer2022-10-23T21:22:09Z2022-10-23T21:22:09Z2022CARVALHO, Caio Eduardo de et al. Inhibition of digestive enzymes (α-amylase, αglucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract. Revista Fitos, v. 16, n. 3, p. 231-247, sept. 2022.1808-9569https://www.arca.fiocruz.br/handle/icict/5525110.32712/2446-4775.2022.12172446-4775engFundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Centro de Inovação em Biodiversidade e SaúdeInhibition of digestive enzymes (α-amylase, αglucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extractInibição de enzimas digestivas (α-amilase, α-glicosidase, lipase, tripsina) pelo extrato aquoso de Hibiscus sabdariffainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Lavras. Faculdade de Ciências da Saúde. Lavras, MG, Brasil.Universidade Federal de Lavras. Instituto de Ciências Naturais. Departamento de Química. Lavras, MG, Brasil.Universidade Federal de Lavras. Faculdade de Ciências da Saúde. Lavras, MG, Brasil.Universidade Federal de Lavras. Instituto de Ciências Naturais. Departamento de Química. Lavras, MG, Brasil.Universidade Federal de Lavras. Instituto de Ciências Naturais. Departamento de Química. Lavras, MG, Brasil.A considerable portion of the world population uses medicinal plants to treat chronic diseases. In this context, hibiscus (Hibiscus sabdariffa L. - Malvaceae) stands out for its supposed hypoglycemic and slimming effect. The present work aimed to carry out enzyme inhibition tests for aqueous hibiscus extracts, as a possible mechanism of action related to the supposed slimming and hypoglycemic effects. The inhibition of four digestive enzymes (α-amylase, α-glucosidase, lipase and trypsin) was tested before and after exposure to a simulated gastric fluid. In addition, a molecular anchorage study was carried out, in order to highlight possible molecular interactions between target and ligand. The results showed that the aqueous extract of hibiscus, in the proportion 1:10 (w/v), inhibits only α-glucosidase. It was observed that cyanidin-3-sambubioside, has interaction with the enzyme with properties similar to acarbose, which corroborates the possibility of the presence of an inhibitory effect in the aqueous extract of hibiscus.Hibiscus sabdariffaGlucosidaseDiabetesObesityMolecular dockinginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/55251/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALc_e_d_et_all.pdfapplication/pdf492855https://www.arca.fiocruz.br/bitstream/icict/55251/2/c_e_d_et_all.pdf20cee3a61c13408263380ee6f46d43f5MD52icict/552512023-02-08 09:09:51.125oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-02-08T12:09:51Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Inhibition of digestive enzymes (α-amylase, αglucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract
dc.title.alternative.pt_BR.fl_str_mv Inibição de enzimas digestivas (α-amilase, α-glicosidase, lipase, tripsina) pelo extrato aquoso de Hibiscus sabdariffa
title Inhibition of digestive enzymes (α-amylase, αglucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract
spellingShingle Inhibition of digestive enzymes (α-amylase, αglucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract
Carvalho, Caio Eduardo de
Hibiscus sabdariffa
Glucosidase
Diabetes
Obesity
Molecular docking
title_short Inhibition of digestive enzymes (α-amylase, αglucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract
title_full Inhibition of digestive enzymes (α-amylase, αglucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract
title_fullStr Inhibition of digestive enzymes (α-amylase, αglucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract
title_full_unstemmed Inhibition of digestive enzymes (α-amylase, αglucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract
title_sort Inhibition of digestive enzymes (α-amylase, αglucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract
author Carvalho, Caio Eduardo de
author_facet Carvalho, Caio Eduardo de
Mancini, Daiana Teixeira
Pereira, Chrystian Araújo
Thomasi, Sérgio Scherrer
author_role author
author2 Mancini, Daiana Teixeira
Pereira, Chrystian Araújo
Thomasi, Sérgio Scherrer
author2_role author
author
author
dc.contributor.author.fl_str_mv Carvalho, Caio Eduardo de
Mancini, Daiana Teixeira
Pereira, Chrystian Araújo
Thomasi, Sérgio Scherrer
dc.subject.en.en_US.fl_str_mv Hibiscus sabdariffa
Glucosidase
Diabetes
Obesity
Molecular docking
topic Hibiscus sabdariffa
Glucosidase
Diabetes
Obesity
Molecular docking
description Universidade Federal de Lavras. Faculdade de Ciências da Saúde. Lavras, MG, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-10-23T21:22:09Z
dc.date.available.fl_str_mv 2022-10-23T21:22:09Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, Caio Eduardo de et al. Inhibition of digestive enzymes (α-amylase, αglucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract. Revista Fitos, v. 16, n. 3, p. 231-247, sept. 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/55251
dc.identifier.issn.none.fl_str_mv 1808-9569
dc.identifier.doi.none.fl_str_mv 10.32712/2446-4775.2022.1217
dc.identifier.eissn.none.fl_str_mv 2446-4775
identifier_str_mv CARVALHO, Caio Eduardo de et al. Inhibition of digestive enzymes (α-amylase, αglucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract. Revista Fitos, v. 16, n. 3, p. 231-247, sept. 2022.
1808-9569
10.32712/2446-4775.2022.1217
2446-4775
url https://www.arca.fiocruz.br/handle/icict/55251
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Centro de Inovação em Biodiversidade e Saúde
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Centro de Inovação em Biodiversidade e Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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