Inhibition of digestive enzymes (α-amylase, α-glucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Revista Fitos |
| Texto Completo: | https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1217 |
Resumo: | A considerable portion of the world population uses medicinal plants to treat chronic diseases. In this context, hibiscus (Hibiscus sabdariffa L. - Malvaceae) stands out for its supposed hypoglycemic and slimming effect. The present work aimed to carry out enzyme inhibition tests for aqueous hibiscus extracts, as a possible mechanism of action related to the supposed slimming and hypoglycemic effects. The inhibition of four digestive enzymes (α-amylase, α-glucosidase, lipase and trypsin) was tested before and after exposure to a simulated gastric fluid. In addition, a molecular anchorage study was carried out, in order to highlight possible molecular interactions between target and ligand. The results showed that the aqueous extract of hibiscus, in the proportion 1:10 (w/v), inhibits only α-glucosidase. It was observed that cyanidin-3-sambubioside, has interaction with the enzyme with properties similar to acarbose, which corroborates the possibility of the presence of an inhibitory effect in the aqueous extract of hibiscus. |
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Inhibition of digestive enzymes (α-amylase, α-glucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extractInibição de enzimas digestivas (α-amilase, α-glicosidase, lipase, tripsina) pelo extrato aquoso de Hibiscus sabdariffaHibiscus sabdariffaGlucosidaseDiabetesObesityMolecular dockingA considerable portion of the world population uses medicinal plants to treat chronic diseases. In this context, hibiscus (Hibiscus sabdariffa L. - Malvaceae) stands out for its supposed hypoglycemic and slimming effect. The present work aimed to carry out enzyme inhibition tests for aqueous hibiscus extracts, as a possible mechanism of action related to the supposed slimming and hypoglycemic effects. The inhibition of four digestive enzymes (α-amylase, α-glucosidase, lipase and trypsin) was tested before and after exposure to a simulated gastric fluid. In addition, a molecular anchorage study was carried out, in order to highlight possible molecular interactions between target and ligand. The results showed that the aqueous extract of hibiscus, in the proportion 1:10 (w/v), inhibits only α-glucosidase. It was observed that cyanidin-3-sambubioside, has interaction with the enzyme with properties similar to acarbose, which corroborates the possibility of the presence of an inhibitory effect in the aqueous extract of hibiscus.Parcela considerável da população mundial faz uso de plantas medicinais no tratamento de doenças crônicas. Neste contexto, o hibisco (Hibiscus sabdariffa) destaca-se pelo suposto efeito hipoglicemiante e emagrecedor. O presente trabalho objetivou a realização de ensaios de inibição enzimática por extratos aquosos de hibisco, como possível mecanismo de ação relacionado aos supostos efeitos emagrecedor e hipoglicemiante. Foi testada a inibição de quatro enzimas digestivas (α-amilase, α-glicosidase, lipase e tripsina), antes e após a exposição a um fluido gástrico simulado. Além disso, foi realizado o estudo de ancoramento molecular, afim de se evidenciar possíveis interações moleculares entre alvo e ligante. Os resultados demonstraram que o extrato aquoso de hibisco, na proporção 1:10 (p:v), inibe apenas a α-glicosidase . Observou-se que a cianidina-3-sambubioside, possui interação com a enzima com propriedades semelhantes à da acarbose o que corrobora a possibilidade da presença de efeito inibitório no extrato aquoso de hibisco.Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz2022-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmltext/xmlhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/121710.32712/2446-4775.2022.1217Revista Fitos; Vol. 16 No. 3 (2022); 315-323Revista Fitos; Vol. 16 Núm. 3 (2022); 315-323Revista Fitos; v. 16 n. 3 (2022); 315-3232446-47751808-9569reponame:Revista Fitosinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZenghttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1217/1060https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1217/1061https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1217/1162Copyright (c) 2022 Revista Fitosinfo:eu-repo/semantics/openAccessCarvalho, Caio Eduardo deMancini, Daiana TeixeiraPereira, Chrystian AraújoThomasi, Sérgio ScherrerPereira, Luciana Lopes SilvaEduardo de Carvalho, Caio2022-09-30T21:09:07Zoai:ojs.revistafitos.far.fiocruz.br:article/1217Revistahttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/PUBhttp://revistafitos.far.fiocruz.br/index.php/revista-fitos/oairevistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br2446-47752446-4775opendoar:2022-09-30T21:09:07Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.none.fl_str_mv |
Inhibition of digestive enzymes (α-amylase, α-glucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract Inibição de enzimas digestivas (α-amilase, α-glicosidase, lipase, tripsina) pelo extrato aquoso de Hibiscus sabdariffa |
| title |
Inhibition of digestive enzymes (α-amylase, α-glucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract |
| spellingShingle |
Inhibition of digestive enzymes (α-amylase, α-glucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract Carvalho, Caio Eduardo de Hibiscus sabdariffa Glucosidase Diabetes Obesity Molecular docking |
| title_short |
Inhibition of digestive enzymes (α-amylase, α-glucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract |
| title_full |
Inhibition of digestive enzymes (α-amylase, α-glucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract |
| title_fullStr |
Inhibition of digestive enzymes (α-amylase, α-glucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract |
| title_full_unstemmed |
Inhibition of digestive enzymes (α-amylase, α-glucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract |
| title_sort |
Inhibition of digestive enzymes (α-amylase, α-glucosidase, lipase, trypsin) by aqueous Hibiscus sabdariffa L. (Malvaceae) extract |
| author |
Carvalho, Caio Eduardo de |
| author_facet |
Carvalho, Caio Eduardo de Mancini, Daiana Teixeira Pereira, Chrystian Araújo Thomasi, Sérgio Scherrer Pereira, Luciana Lopes Silva Eduardo de Carvalho, Caio |
| author_role |
author |
| author2 |
Mancini, Daiana Teixeira Pereira, Chrystian Araújo Thomasi, Sérgio Scherrer Pereira, Luciana Lopes Silva Eduardo de Carvalho, Caio |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Carvalho, Caio Eduardo de Mancini, Daiana Teixeira Pereira, Chrystian Araújo Thomasi, Sérgio Scherrer Pereira, Luciana Lopes Silva Eduardo de Carvalho, Caio |
| dc.subject.por.fl_str_mv |
Hibiscus sabdariffa Glucosidase Diabetes Obesity Molecular docking |
| topic |
Hibiscus sabdariffa Glucosidase Diabetes Obesity Molecular docking |
| description |
A considerable portion of the world population uses medicinal plants to treat chronic diseases. In this context, hibiscus (Hibiscus sabdariffa L. - Malvaceae) stands out for its supposed hypoglycemic and slimming effect. The present work aimed to carry out enzyme inhibition tests for aqueous hibiscus extracts, as a possible mechanism of action related to the supposed slimming and hypoglycemic effects. The inhibition of four digestive enzymes (α-amylase, α-glucosidase, lipase and trypsin) was tested before and after exposure to a simulated gastric fluid. In addition, a molecular anchorage study was carried out, in order to highlight possible molecular interactions between target and ligand. The results showed that the aqueous extract of hibiscus, in the proportion 1:10 (w/v), inhibits only α-glucosidase. It was observed that cyanidin-3-sambubioside, has interaction with the enzyme with properties similar to acarbose, which corroborates the possibility of the presence of an inhibitory effect in the aqueous extract of hibiscus. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-09-30 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1217 10.32712/2446-4775.2022.1217 |
| url |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1217 |
| identifier_str_mv |
10.32712/2446-4775.2022.1217 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1217/1060 https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1217/1061 https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1217/1162 |
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Copyright (c) 2022 Revista Fitos info:eu-repo/semantics/openAccess |
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Copyright (c) 2022 Revista Fitos |
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openAccess |
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application/pdf text/html text/xml |
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Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz |
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Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz |
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Revista Fitos; Vol. 16 No. 3 (2022); 315-323 Revista Fitos; Vol. 16 Núm. 3 (2022); 315-323 Revista Fitos; v. 16 n. 3 (2022); 315-323 2446-4775 1808-9569 reponame:Revista Fitos instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Fundação Oswaldo Cruz (FIOCRUZ) |
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FIOCRUZ |
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Revista Fitos |
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Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ) |
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revistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br |
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