Inibição de enzimas digestivas (α-amilase, α-glicosidase, lipase, tripsina) pelo extrato aquoso de Hibiscus sabdariffa

Detalhes bibliográficos
Autor(a) principal: Carvalho, Caio Eduardo de
Data de Publicação: 2022
Outros Autores: Mancini, Daiana Teixeira, Pereira, Chrystian Araújo, Thomasi, Sérgio Scherrer, Pereira, Luciana Lopes Silva
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/55228
Resumo: A considerable portion of the world population uses medicinal plants to treat chronic diseases. In this context, hibiscus (Hibiscus sabdariffa L. - Malvaceae) stands out for its supposed hypoglycemic and slimming effect. The present work aimed to carry out enzyme inhibition tests for aqueous hibiscus extracts, as a possible mechanism of action related to the supposed slimming and hypoglycemic effects. The inhibition of four digestive enzymes (α-amylase, α-glucosidase, lipase and trypsin) was tested before and after exposure to a simulated gastric fluid. In addition, a molecular anchorage study was carried out, in order to highlight possible molecular interactions between target and ligand. The results showed that the aqueous extract of hibiscus, in the proportion 1:10 (w/v), inhibits only α-glucosidase. It was observed that cyanidin-3- sambubioside, has interaction with the enzyme with properties similar to acarbose, which corroborates the possibility of the presence of an inhibitory effect in the aqueous extract of hibiscus.
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spelling Inibição de enzimas digestivas (α-amilase, α-glicosidase, lipase, tripsina) pelo extrato aquoso de Hibiscus sabdariffaHibiscus sabdariffaGlucosidaseMolecular dockingDiabetesObesityObesidadeA considerable portion of the world population uses medicinal plants to treat chronic diseases. In this context, hibiscus (Hibiscus sabdariffa L. - Malvaceae) stands out for its supposed hypoglycemic and slimming effect. The present work aimed to carry out enzyme inhibition tests for aqueous hibiscus extracts, as a possible mechanism of action related to the supposed slimming and hypoglycemic effects. The inhibition of four digestive enzymes (α-amylase, α-glucosidase, lipase and trypsin) was tested before and after exposure to a simulated gastric fluid. In addition, a molecular anchorage study was carried out, in order to highlight possible molecular interactions between target and ligand. The results showed that the aqueous extract of hibiscus, in the proportion 1:10 (w/v), inhibits only α-glucosidase. It was observed that cyanidin-3- sambubioside, has interaction with the enzyme with properties similar to acarbose, which corroborates the possibility of the presence of an inhibitory effect in the aqueous extract of hibiscus.Parcela considerável da população mundial faz uso de plantas medicinais no tratamento de doenças crônicas. Neste contexto, o hibisco (Hibiscus sabdariffa) destaca-se pelo suposto efeito hipoglicemiante e emagrecedor. O presente trabalho objetivou a realização de ensaios de inibição enzimática por extratos aquosos de hibisco, como possível mecanismo de ação relacionado aos supostos efeitos emagrecedor e hipoglicemiante. Foi testada a inibição de quatro enzimas digestivas (α-amilase, α-glicosidase, lipase e tripsina), antes e após a exposição a um fluido gástrico simulado. Além disso, foi realizado o estudo de ancoramento molecular, afim de se evidenciar possíveis interações moleculares entre alvo e ligante. Os resultados demonstraram que o extrato aquoso de hibisco, na proporção 1:10 (p:v), inibe apenas a α-glicosidase . Observou-se que a cianidina-3-sambubioside, possui interação com a enzima com propriedades semelhantes à da acarbose o que corrobora a possibilidade da presença de efeito inibitório no extrato aquoso de hibisco.Fundação Oswaldo Cruz2022-09-28T18:20:54Z2022-09-28T18:20:54Z2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCARVALHO, C. E. de et al. Inibição de enzimas digestivas (α-amilase, α-glicosidase, lipase, tripsina) pelo extrato aquoso de Hibiscus sabdariffa. Revista Fitos, Rio de Janeiro, 2022.http://repositorio.ufla.br/jspui/handle/1/55228Revista Fitosreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessCarvalho, Caio Eduardo deMancini, Daiana TeixeiraPereira, Chrystian AraújoThomasi, Sérgio ScherrerPereira, Luciana Lopes Silvapor2022-09-28T18:20:54Zoai:localhost:1/55228Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2022-09-28T18:20:54Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Inibição de enzimas digestivas (α-amilase, α-glicosidase, lipase, tripsina) pelo extrato aquoso de Hibiscus sabdariffa
title Inibição de enzimas digestivas (α-amilase, α-glicosidase, lipase, tripsina) pelo extrato aquoso de Hibiscus sabdariffa
spellingShingle Inibição de enzimas digestivas (α-amilase, α-glicosidase, lipase, tripsina) pelo extrato aquoso de Hibiscus sabdariffa
Carvalho, Caio Eduardo de
Hibiscus sabdariffa
Glucosidase
Molecular docking
Diabetes
Obesity
Obesidade
title_short Inibição de enzimas digestivas (α-amilase, α-glicosidase, lipase, tripsina) pelo extrato aquoso de Hibiscus sabdariffa
title_full Inibição de enzimas digestivas (α-amilase, α-glicosidase, lipase, tripsina) pelo extrato aquoso de Hibiscus sabdariffa
title_fullStr Inibição de enzimas digestivas (α-amilase, α-glicosidase, lipase, tripsina) pelo extrato aquoso de Hibiscus sabdariffa
title_full_unstemmed Inibição de enzimas digestivas (α-amilase, α-glicosidase, lipase, tripsina) pelo extrato aquoso de Hibiscus sabdariffa
title_sort Inibição de enzimas digestivas (α-amilase, α-glicosidase, lipase, tripsina) pelo extrato aquoso de Hibiscus sabdariffa
author Carvalho, Caio Eduardo de
author_facet Carvalho, Caio Eduardo de
Mancini, Daiana Teixeira
Pereira, Chrystian Araújo
Thomasi, Sérgio Scherrer
Pereira, Luciana Lopes Silva
author_role author
author2 Mancini, Daiana Teixeira
Pereira, Chrystian Araújo
Thomasi, Sérgio Scherrer
Pereira, Luciana Lopes Silva
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Caio Eduardo de
Mancini, Daiana Teixeira
Pereira, Chrystian Araújo
Thomasi, Sérgio Scherrer
Pereira, Luciana Lopes Silva
dc.subject.por.fl_str_mv Hibiscus sabdariffa
Glucosidase
Molecular docking
Diabetes
Obesity
Obesidade
topic Hibiscus sabdariffa
Glucosidase
Molecular docking
Diabetes
Obesity
Obesidade
description A considerable portion of the world population uses medicinal plants to treat chronic diseases. In this context, hibiscus (Hibiscus sabdariffa L. - Malvaceae) stands out for its supposed hypoglycemic and slimming effect. The present work aimed to carry out enzyme inhibition tests for aqueous hibiscus extracts, as a possible mechanism of action related to the supposed slimming and hypoglycemic effects. The inhibition of four digestive enzymes (α-amylase, α-glucosidase, lipase and trypsin) was tested before and after exposure to a simulated gastric fluid. In addition, a molecular anchorage study was carried out, in order to highlight possible molecular interactions between target and ligand. The results showed that the aqueous extract of hibiscus, in the proportion 1:10 (w/v), inhibits only α-glucosidase. It was observed that cyanidin-3- sambubioside, has interaction with the enzyme with properties similar to acarbose, which corroborates the possibility of the presence of an inhibitory effect in the aqueous extract of hibiscus.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-28T18:20:54Z
2022-09-28T18:20:54Z
2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv CARVALHO, C. E. de et al. Inibição de enzimas digestivas (α-amilase, α-glicosidase, lipase, tripsina) pelo extrato aquoso de Hibiscus sabdariffa. Revista Fitos, Rio de Janeiro, 2022.
http://repositorio.ufla.br/jspui/handle/1/55228
identifier_str_mv CARVALHO, C. E. de et al. Inibição de enzimas digestivas (α-amilase, α-glicosidase, lipase, tripsina) pelo extrato aquoso de Hibiscus sabdariffa. Revista Fitos, Rio de Janeiro, 2022.
url http://repositorio.ufla.br/jspui/handle/1/55228
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv Revista Fitos
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1807835149123977216

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