Sickle red cells as danger signals on proinflammatory gene expression, leukotriene B4 and interleukin-1 beta production in peripheral blood mononuclear cell
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | dx.doi.org/10.1016/j.cyto.2016.03.016 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/14136 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil |
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Pitanga, Thassila NogueiraOliveira, Ricardo RiccioZanette, Dalila LucíolaGuarda, Caroline Conceição daSantiago, Rayra PereiraSantana, Sanzio SilvaNascimento, Valma M. LLima, Jonilson BerlinkCarvalho, Graziele QuintelaMaffili, Vitor ValérioCarvalho, Magda Oliveira SeixasAlcantara, Luiz Carlos JúniorBorges, Valéria de MatosGoncalves, Marilda de Souza2016-05-09T18:29:16Z2016-05-09T18:29:16Z2016PITANGA, T. N. et al. Sickle red cells as danger signals on proinflammatory gene expression, leukotriene B4 and interleukin-1 beta production in peripheral blood mononuclear cell. Cytokine, v. 83, p. 75–84, 2016.1043-4666https://www.arca.fiocruz.br/handle/icict/14136dx.doi.org/10.1016/j.cyto.2016.03.016engElsevierSickle red cells as danger signals on proinflammatory gene expression, leukotriene B4 and interleukin-1 beta production in peripheral blood mononuclear cellinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação de Hematologia e Hemoterapia da Bahia. HEMOBA. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital Universitário Professor Edgard Santos. HUPES. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia, Salvador, BA, BrasilThis study tested the hypothesis that sickle red blood cell (SS-RBC) induce Toll-like receptors (TLR) and Nod-like receptor family, pyrin domain containing 3 (NLRP3)- inflammasome expression in peripheral blood mononuclear cells (PBMC). TLR and NLRP3 inflammasome could contribute to the maintenance of the inflammatory status in sickle cell anemia (SCA) patients, since SS-RBC act as danger signals activating these pathways. In this study, first, we evaluated TLR (2, 4, 5 and 9), NLRP3, Caspase-1, interleukin (IL)- 1b and IL-18 expression in PBMC freshly isolated from SCA patients (SS-PBMC) in comparison with PBMC from healthy individuals (AA-PBMC). In the second moment, we investigated whether SS-RBC could interfere with the expression of these molecules in PBMC from healthy donor, in the absence or presence of hydroxyurea (HU) in vitro. TLRs and NLRP3 inflammasome expression were investigated by qPCR. IL- 1b, Leukotriene-B4 (LTB4) and nitrite production were measured in PBMC (from healthy donor) culture supernatants. TLR2, TLR4, TLR5, NLRP3 and IL-1b were highly expressed in SS-PBMC when compared to AA-PBMC. Additionally, SS-RBC induced TLR9, NLRP3, Caspase-1, IL-1b and IL-18 expression and induced IL-1b, LTB4 and nitrite production in PBMC cultures. HU did not prevent TLR and NLRP3 inflammasome expression, but increased TLR2 and IL-18 expression and reduced nitrite production. In conclusion, our data suggest that TLR and inflammasome complexes may be key inducers of inflammation in SCA patients, probably through SS-RBC; also, HU does not prevent NLRP3 inflammasome- and TLR-dependent inflammation, indicating the need to develop new therapeutic strategies to SCA patients that act with different mechanisms of those observed for HU.Sickle cell anemiaSickle red cellIL-1BLeukotriene B4NLRP3 inflammasomeToll-like receptorsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/14136/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPitanga TN Sickle red....pdfPitanga TN Sickle red....pdfapplication/pdf1423177https://www.arca.fiocruz.br/bitstream/icict/14136/2/Pitanga%20TN%20Sickle%20red....pdf1193d7c6c6ba17a60060934f754028d3MD52TEXTPitanga TN Sickle red....pdf.txtPitanga TN Sickle red....pdf.txtExtracted texttext/plain54873https://www.arca.fiocruz.br/bitstream/icict/14136/3/Pitanga%20TN%20Sickle%20red....pdf.txtfe8499d80402a41f1740ff855520cf45MD53icict/141362023-03-15 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| dc.title.pt_BR.fl_str_mv |
Sickle red cells as danger signals on proinflammatory gene expression, leukotriene B4 and interleukin-1 beta production in peripheral blood mononuclear cell |
| title |
Sickle red cells as danger signals on proinflammatory gene expression, leukotriene B4 and interleukin-1 beta production in peripheral blood mononuclear cell |
| spellingShingle |
Sickle red cells as danger signals on proinflammatory gene expression, leukotriene B4 and interleukin-1 beta production in peripheral blood mononuclear cell Pitanga, Thassila Nogueira Sickle cell anemia Sickle red cell IL-1B Leukotriene B4 NLRP3 inflammasome Toll-like receptors |
| title_short |
Sickle red cells as danger signals on proinflammatory gene expression, leukotriene B4 and interleukin-1 beta production in peripheral blood mononuclear cell |
| title_full |
Sickle red cells as danger signals on proinflammatory gene expression, leukotriene B4 and interleukin-1 beta production in peripheral blood mononuclear cell |
| title_fullStr |
Sickle red cells as danger signals on proinflammatory gene expression, leukotriene B4 and interleukin-1 beta production in peripheral blood mononuclear cell |
| title_full_unstemmed |
Sickle red cells as danger signals on proinflammatory gene expression, leukotriene B4 and interleukin-1 beta production in peripheral blood mononuclear cell |
| title_sort |
Sickle red cells as danger signals on proinflammatory gene expression, leukotriene B4 and interleukin-1 beta production in peripheral blood mononuclear cell |
| author |
Pitanga, Thassila Nogueira |
| author_facet |
Pitanga, Thassila Nogueira Oliveira, Ricardo Riccio Zanette, Dalila Lucíola Guarda, Caroline Conceição da Santiago, Rayra Pereira Santana, Sanzio Silva Nascimento, Valma M. L Lima, Jonilson Berlink Carvalho, Graziele Quintela Maffili, Vitor Valério Carvalho, Magda Oliveira Seixas Alcantara, Luiz Carlos Júnior Borges, Valéria de Matos Goncalves, Marilda de Souza |
| author_role |
author |
| author2 |
Oliveira, Ricardo Riccio Zanette, Dalila Lucíola Guarda, Caroline Conceição da Santiago, Rayra Pereira Santana, Sanzio Silva Nascimento, Valma M. L Lima, Jonilson Berlink Carvalho, Graziele Quintela Maffili, Vitor Valério Carvalho, Magda Oliveira Seixas Alcantara, Luiz Carlos Júnior Borges, Valéria de Matos Goncalves, Marilda de Souza |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Pitanga, Thassila Nogueira Oliveira, Ricardo Riccio Zanette, Dalila Lucíola Guarda, Caroline Conceição da Santiago, Rayra Pereira Santana, Sanzio Silva Nascimento, Valma M. L Lima, Jonilson Berlink Carvalho, Graziele Quintela Maffili, Vitor Valério Carvalho, Magda Oliveira Seixas Alcantara, Luiz Carlos Júnior Borges, Valéria de Matos Goncalves, Marilda de Souza |
| dc.subject.en.pt_BR.fl_str_mv |
Sickle cell anemia Sickle red cell IL-1B Leukotriene B4 NLRP3 inflammasome Toll-like receptors |
| topic |
Sickle cell anemia Sickle red cell IL-1B Leukotriene B4 NLRP3 inflammasome Toll-like receptors |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil |
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2016 |
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2016-05-09T18:29:16Z |
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2016-05-09T18:29:16Z |
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2016 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
PITANGA, T. N. et al. Sickle red cells as danger signals on proinflammatory gene expression, leukotriene B4 and interleukin-1 beta production in peripheral blood mononuclear cell. Cytokine, v. 83, p. 75–84, 2016. |
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https://www.arca.fiocruz.br/handle/icict/14136 |
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1043-4666 |
| dc.identifier.doi.none.fl_str_mv |
dx.doi.org/10.1016/j.cyto.2016.03.016 |
| identifier_str_mv |
PITANGA, T. N. et al. Sickle red cells as danger signals on proinflammatory gene expression, leukotriene B4 and interleukin-1 beta production in peripheral blood mononuclear cell. Cytokine, v. 83, p. 75–84, 2016. 1043-4666 dx.doi.org/10.1016/j.cyto.2016.03.016 |
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https://www.arca.fiocruz.br/handle/icict/14136 |
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eng |
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Elsevier |
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Elsevier |
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