Combined occurrence of trypanosomal sialidase/trans-sialidase activities and leishmanial metalloproteinase gene homologues in Endotrypanum sp.

Detalhes bibliográficos
Autor(a) principal: Acosta, Enrique Medina
Data de Publicação: 1994
Outros Autores: Paul, Simon, Tomlinson, Stephen, Pontes-de-Carvalho, Lain Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8335
Resumo: The Rockfeller University. New York, NY, USA
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spelling Acosta, Enrique MedinaPaul, SimonTomlinson, StephenPontes-de-Carvalho, Lain Carlos2014-09-09T17:16:26Z2014-09-09T17:16:26Z1994MEDINA-ACOSTA, E. et al. Combined occurrence of trypanosomal sialidase/trans-sialidase activities and leishmanial metalloproteinase gene homologues in Endotrypanum sp. Molecular Biochemical Parasitology, v. 64, n. 2, p. 273-282, 1994.0166-6851https://www.arca.fiocruz.br/handle/icict/8335engElsevierCombined occurrence of trypanosomal sialidase/trans-sialidase activities and leishmanial metalloproteinase gene homologues in Endotrypanum sp.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleThe Rockfeller University. New York, NY, USAThe Rockfeller University. New York, NY, USANew York University Medical Center. New York, NY, USANew York University Medical Center. New York, NY, USA / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilEndotrypanum (order Kinetoplastida: family Trypanosomatidae) is a parasite of forest dwelling tree sloths (Edentata: genera Choleopus and Bradypus). Unique among the haemoflagellates, this protozoan has an intraerythrocytic phase in the mammalian host. Nevertheless, many striking similarities exist between Endotrypanum and the human pathogen Leishmania that make it a useful model for epidemiological and evolutionary aspects of the biology of trypanosomatids. Importantly, Endotrypanum species share both the insect vector and host reservoir with certain species of Leishmania (subgenus Viannia). Because mixed infections with Endotrypanum and Leishmania are common in sloths and, therefore, likely to occur in the sandfly vector, there is a need for adequate biochemical markers to distinguish Endotrypanum from Leishmania infections. In this paper we show that Endotrypanum promastigotes possess sialidase and trans-sialidase activities, which are absent from Leishmania, and which are not closely related to the previously described trypanosomal sialidase/trans-sialidase enzyme. We also document the occurrence in Endotrypanum of homologues of the leishmanial surface metalloproteinase gp63 genes. The combined occurrence of sialidase/trans-sialidase activities and gp63 gene homologues in a unique organism has important ramifications for both field and laboratory studies on the biology of trypanosomatids, especially those related to host infection and evolutionEndototrypanumKinetoplastidMetalloproteinaseNeuraminidaseTrans-sialidaseSlothMetaloendopeptidases/genéticaNeuraminidase/genéticaTrypanosomatina/enzimologiaTrypanosomatina/genéticaAnimaisEvolução BiológicaDNA de Protozoário/genéticaGenes de ProtozoáriosGlicoproteínas/genéticaLeishmania/enzimologiaLeishmania/genéticaHibridização de Ácido NucleicoEspecificidade da EspécieTrypanosoma/enzimologiaTrypanosoma/genéticaTrypanosomatina/classificaçãoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8335/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALMedina-Acosta E Trans-sialidase....pdfMedina-Acosta E Trans-sialidase....pdfapplication/pdf806326https://www.arca.fiocruz.br/bitstream/icict/8335/2/Medina-Acosta%20E%20Trans-sialidase....pdf6de64d4d093dee46c5513925f2f8d3e6MD52TEXTMedina-Acosta E Trans-sialidase....pdf.txtMedina-Acosta E Trans-sialidase....pdf.txtExtracted texttext/plain38384https://www.arca.fiocruz.br/bitstream/icict/8335/3/Medina-Acosta%20E%20Trans-sialidase....pdf.txt354c26dfc8d9dab1bc4a67b355894b25MD53icict/83352023-03-15 14:33:24.124oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:24Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Combined occurrence of trypanosomal sialidase/trans-sialidase activities and leishmanial metalloproteinase gene homologues in Endotrypanum sp.
title Combined occurrence of trypanosomal sialidase/trans-sialidase activities and leishmanial metalloproteinase gene homologues in Endotrypanum sp.
spellingShingle Combined occurrence of trypanosomal sialidase/trans-sialidase activities and leishmanial metalloproteinase gene homologues in Endotrypanum sp.
Acosta, Enrique Medina
Endototrypanum
Kinetoplastid
Metalloproteinase
Neuraminidase
Trans-sialidase
Sloth
Metaloendopeptidases/genética
Neuraminidase/genética
Trypanosomatina/enzimologia
Trypanosomatina/genética
Animais
Evolução Biológica
DNA de Protozoário/genética
Genes de Protozoários
Glicoproteínas/genética
Leishmania/enzimologia
Leishmania/genética
Hibridização de Ácido Nucleico
Especificidade da Espécie
Trypanosoma/enzimologia
Trypanosoma/genética
Trypanosomatina/classificação
title_short Combined occurrence of trypanosomal sialidase/trans-sialidase activities and leishmanial metalloproteinase gene homologues in Endotrypanum sp.
title_full Combined occurrence of trypanosomal sialidase/trans-sialidase activities and leishmanial metalloproteinase gene homologues in Endotrypanum sp.
title_fullStr Combined occurrence of trypanosomal sialidase/trans-sialidase activities and leishmanial metalloproteinase gene homologues in Endotrypanum sp.
title_full_unstemmed Combined occurrence of trypanosomal sialidase/trans-sialidase activities and leishmanial metalloproteinase gene homologues in Endotrypanum sp.
title_sort Combined occurrence of trypanosomal sialidase/trans-sialidase activities and leishmanial metalloproteinase gene homologues in Endotrypanum sp.
author Acosta, Enrique Medina
author_facet Acosta, Enrique Medina
Paul, Simon
Tomlinson, Stephen
Pontes-de-Carvalho, Lain Carlos
author_role author
author2 Paul, Simon
Tomlinson, Stephen
Pontes-de-Carvalho, Lain Carlos
author2_role author
author
author
dc.contributor.author.fl_str_mv Acosta, Enrique Medina
Paul, Simon
Tomlinson, Stephen
Pontes-de-Carvalho, Lain Carlos
dc.subject.en.pt_BR.fl_str_mv Endototrypanum
Kinetoplastid
Metalloproteinase
Neuraminidase
Trans-sialidase
Sloth
topic Endototrypanum
Kinetoplastid
Metalloproteinase
Neuraminidase
Trans-sialidase
Sloth
Metaloendopeptidases/genética
Neuraminidase/genética
Trypanosomatina/enzimologia
Trypanosomatina/genética
Animais
Evolução Biológica
DNA de Protozoário/genética
Genes de Protozoários
Glicoproteínas/genética
Leishmania/enzimologia
Leishmania/genética
Hibridização de Ácido Nucleico
Especificidade da Espécie
Trypanosoma/enzimologia
Trypanosoma/genética
Trypanosomatina/classificação
dc.subject.decs.pt_BR.fl_str_mv Metaloendopeptidases/genética
Neuraminidase/genética
Trypanosomatina/enzimologia
Trypanosomatina/genética
Animais
Evolução Biológica
DNA de Protozoário/genética
Genes de Protozoários
Glicoproteínas/genética
Leishmania/enzimologia
Leishmania/genética
Hibridização de Ácido Nucleico
Especificidade da Espécie
Trypanosoma/enzimologia
Trypanosoma/genética
Trypanosomatina/classificação
description The Rockfeller University. New York, NY, USA
publishDate 1994
dc.date.issued.fl_str_mv 1994
dc.date.accessioned.fl_str_mv 2014-09-09T17:16:26Z
dc.date.available.fl_str_mv 2014-09-09T17:16:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MEDINA-ACOSTA, E. et al. Combined occurrence of trypanosomal sialidase/trans-sialidase activities and leishmanial metalloproteinase gene homologues in Endotrypanum sp. Molecular Biochemical Parasitology, v. 64, n. 2, p. 273-282, 1994.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8335
dc.identifier.issn.none.fl_str_mv 0166-6851
identifier_str_mv MEDINA-ACOSTA, E. et al. Combined occurrence of trypanosomal sialidase/trans-sialidase activities and leishmanial metalloproteinase gene homologues in Endotrypanum sp. Molecular Biochemical Parasitology, v. 64, n. 2, p. 273-282, 1994.
0166-6851
url https://www.arca.fiocruz.br/handle/icict/8335
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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