Trans-sialidase and sialidase activities discriminate between morphologically indistinguishable trypanosomatids.

Detalhes bibliográficos
Autor(a) principal: Acosta, Enrique Medina
Data de Publicação: 1994
Outros Autores: Pereira, Antonia Maria Ramos Franco, Jansen, Ana Maria, Sampol, Marcos, Neves, Neuza Maria Alcântara, Pontes-de-Carvalho, Lain Carlos, Grimaldi Junior, Gabriel, Nussenzweig, Victor
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8336
Resumo: New York University Medical Center. Michael Heidelberger Division of Immunology. New York, USA
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spelling Acosta, Enrique MedinaPereira, Antonia Maria Ramos FrancoJansen, Ana MariaSampol, MarcosNeves, Neuza Maria AlcântaraPontes-de-Carvalho, Lain CarlosGrimaldi Junior, GabrielNussenzweig, Victor2014-09-09T18:08:39Z2014-09-09T18:08:39Z1994MEDINA-ACOSTA, E. et al. Trans-sialidase and sialidase activities discriminate between morphologically indistinguishable trypanosomatids. European Journal Biochemical, v. 225, n. 1, p. 333-339, 1994.0014-2956https://www.arca.fiocruz.br/handle/icict/8336engFEBSTrans-sialidase and sialidase activities discriminate between morphologically indistinguishable trypanosomatids.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNew York University Medical Center. Michael Heidelberger Division of Immunology. New York, USAFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilNew York University Medical Center. Michael Heidelberger Division of Immunology. New York, USA / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilNew York University Medical Center. Michael Heidelberger Division of Immunology. New York, USA / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilNew York University Medical Center. Michael Heidelberger Division of Immunology. New York, USA / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilNew York University Medical Center. Michael Heidelberger Division of Immunology. New York, USAThe expression of trans-sialidase and sialidase activities in the kinetoplastid protozoa was explored as a potential marker to discriminate between the morphologically indistinguishable flagellates isolated from human, insects and vertebrate reservoir hosts. By virtue of the differences observed in the ratios of these enzyme activities, a collection of 52 species and strains comprising the major taxa of these parasites could be separated into four expression types. Type-I parasites express comparable levels of both trans-sialidase and sialidase activities (Endotrypanum species and Trypanosoma lewisi). Type-I1 parasites express predominantly trans-sialidase activity (Trypanosoma cruzi and Trypanosoma conorhini).Type-I11 parasites express sialidase activity exclusively (Trypanosoma rangeli and Trypanosoma leeuwenhoeki). Type-IV parasites do not express either activity (Leishmania species and Trypanoplasma borreli). The measurement of trans-sialidase and sialidase activities thus permits the differentiation of parasites frequently found in the same insect vectors that are difficult to distinguish, such as T. cruzi and 7: rangeli, or in the same sylvatic vertebrate and invertebrate hosts, such as Leishmania and Endotrypanum.Neuraminidase/análiseSialiltransferases/análiseTrypanosoma/classificaçãoTrypanosomatina/classificaçãoAnimaisDNA de Protozoário/isolamento & purificaçãoReservatórios de DoençasHumanosInsetosNeuraminidase/biossínteseReação em Cadeia da PolimeraseSialiltransferases/biossínteseTrypanosoma/isolamento & purificaçãoTrypanosomatina/isolamento & purificaçãoVertebradosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8336/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALMedina-Acosta E Trans-sialidase....pdfMedina-Acosta E Trans-sialidase....pdfapplication/pdf806326https://www.arca.fiocruz.br/bitstream/icict/8336/2/Medina-Acosta%20E%20Trans-sialidase....pdf6de64d4d093dee46c5513925f2f8d3e6MD52TEXTMedina-Acosta E Trans-sialidase....pdf.txtMedina-Acosta E Trans-sialidase....pdf.txtExtracted texttext/plain38384https://www.arca.fiocruz.br/bitstream/icict/8336/3/Medina-Acosta%20E%20Trans-sialidase....pdf.txt354c26dfc8d9dab1bc4a67b355894b25MD53icict/83362023-03-15 14:33:24.423oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:24Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Trans-sialidase and sialidase activities discriminate between morphologically indistinguishable trypanosomatids.
title Trans-sialidase and sialidase activities discriminate between morphologically indistinguishable trypanosomatids.
spellingShingle Trans-sialidase and sialidase activities discriminate between morphologically indistinguishable trypanosomatids.
Acosta, Enrique Medina
Neuraminidase/análise
Sialiltransferases/análise
Trypanosoma/classificação
Trypanosomatina/classificação
Animais
DNA de Protozoário/isolamento & purificação
Reservatórios de Doenças
Humanos
Insetos
Neuraminidase/biossíntese
Reação em Cadeia da Polimerase
Sialiltransferases/biossíntese
Trypanosoma/isolamento & purificação
Trypanosomatina/isolamento & purificação
Vertebrados
title_short Trans-sialidase and sialidase activities discriminate between morphologically indistinguishable trypanosomatids.
title_full Trans-sialidase and sialidase activities discriminate between morphologically indistinguishable trypanosomatids.
title_fullStr Trans-sialidase and sialidase activities discriminate between morphologically indistinguishable trypanosomatids.
title_full_unstemmed Trans-sialidase and sialidase activities discriminate between morphologically indistinguishable trypanosomatids.
title_sort Trans-sialidase and sialidase activities discriminate between morphologically indistinguishable trypanosomatids.
author Acosta, Enrique Medina
author_facet Acosta, Enrique Medina
Pereira, Antonia Maria Ramos Franco
Jansen, Ana Maria
Sampol, Marcos
Neves, Neuza Maria Alcântara
Pontes-de-Carvalho, Lain Carlos
Grimaldi Junior, Gabriel
Nussenzweig, Victor
author_role author
author2 Pereira, Antonia Maria Ramos Franco
Jansen, Ana Maria
Sampol, Marcos
Neves, Neuza Maria Alcântara
Pontes-de-Carvalho, Lain Carlos
Grimaldi Junior, Gabriel
Nussenzweig, Victor
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Acosta, Enrique Medina
Pereira, Antonia Maria Ramos Franco
Jansen, Ana Maria
Sampol, Marcos
Neves, Neuza Maria Alcântara
Pontes-de-Carvalho, Lain Carlos
Grimaldi Junior, Gabriel
Nussenzweig, Victor
dc.subject.decs.pt_BR.fl_str_mv Neuraminidase/análise
Sialiltransferases/análise
Trypanosoma/classificação
Trypanosomatina/classificação
Animais
DNA de Protozoário/isolamento & purificação
Reservatórios de Doenças
Humanos
Insetos
Neuraminidase/biossíntese
Reação em Cadeia da Polimerase
Sialiltransferases/biossíntese
Trypanosoma/isolamento & purificação
Trypanosomatina/isolamento & purificação
Vertebrados
topic Neuraminidase/análise
Sialiltransferases/análise
Trypanosoma/classificação
Trypanosomatina/classificação
Animais
DNA de Protozoário/isolamento & purificação
Reservatórios de Doenças
Humanos
Insetos
Neuraminidase/biossíntese
Reação em Cadeia da Polimerase
Sialiltransferases/biossíntese
Trypanosoma/isolamento & purificação
Trypanosomatina/isolamento & purificação
Vertebrados
description New York University Medical Center. Michael Heidelberger Division of Immunology. New York, USA
publishDate 1994
dc.date.issued.fl_str_mv 1994
dc.date.accessioned.fl_str_mv 2014-09-09T18:08:39Z
dc.date.available.fl_str_mv 2014-09-09T18:08:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MEDINA-ACOSTA, E. et al. Trans-sialidase and sialidase activities discriminate between morphologically indistinguishable trypanosomatids. European Journal Biochemical, v. 225, n. 1, p. 333-339, 1994.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8336
dc.identifier.issn.none.fl_str_mv 0014-2956
identifier_str_mv MEDINA-ACOSTA, E. et al. Trans-sialidase and sialidase activities discriminate between morphologically indistinguishable trypanosomatids. European Journal Biochemical, v. 225, n. 1, p. 333-339, 1994.
0014-2956
url https://www.arca.fiocruz.br/handle/icict/8336
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv FEBS
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