Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation.

Detalhes bibliográficos
Autor(a) principal: Duarte, Tonya Azevedo
Data de Publicação: 2012
Outros Autores: Dutra, Alberto Augusto Noronha, Nery, Joilda Silva, Ribeiro, Samantha Brum, Pitanga, Thassila Nogueira, Silva, José R. Lapa e, Arruda, Sérgio Marcos, Boéchat, Neio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8802
Resumo: Federal University of Bahia. Health Science Institute. Salvador, BA, Brasil / Federal University of Rio de Janeiro. Clementino Fraga Filho University Hospital, Department of Internal Medicine. Multidisciplinary Research Laboratory. Rio de Janeiro, RJ, Brasil
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spelling Duarte, Tonya AzevedoDutra, Alberto Augusto NoronhaNery, Joilda SilvaRibeiro, Samantha BrumPitanga, Thassila NogueiraSilva, José R. Lapa eArruda, Sérgio MarcosBoéchat, Neio2014-11-12T14:22:42Z2014-11-12T14:22:42Z2012DUARTE, T. A. et al. Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation. Tuberculosis (Edinb), v. 92, n. 3, p. 218-225, 2012.1873-281Xhttps://www.arca.fiocruz.br/handle/icict/880210.1016/j.tube.2012.02.007engElsevier LtdMycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFederal University of Bahia. Health Science Institute. Salvador, BA, Brasil / Federal University of Rio de Janeiro. Clementino Fraga Filho University Hospital, Department of Internal Medicine. Multidisciplinary Research Laboratory. Rio de Janeiro, RJ, BrasilUniversity College London. Gower Street, London, UK / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFederal University of Rio de Janeiro. Clementino Fraga Filho University Hospital, Department of Internal Medicine. Multidisciplinary Research Laboratory. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFederal University of Rio de Janeiro. Clementino Fraga Filho University Hospital, Department of Internal Medicine. Multidisciplinary Research Laboratory. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFederal University of Rio de Janeiro. Clementino Fraga Filho University Hospital, Department of Internal Medicine. Multidisciplinary Research Laboratory. Rio de Janeiro, RJ, BrasilBACKGROUND: The existence of ectosome-like microvesicles released by neutrophils was proposed a few decades ago. Other studies revealed that the innate immune response during mycobacterial infection is accompanied by an intense migration of neutrophils to the site of infection, which may be important during the acute phase of tuberculosis. We found that the ectosomes derived from infected neutrophils are biologically active and can influence the survival of Mycobacterium tuberculosis within macrophages. METHODS: Mycobacteria were cultured on supplemented Middlebrook-7H9 broth. All strains were grown to the exponential phase and quantitated by serial dilution. Human neutrophils and macrophages were infected with mycobacteria. Ectosomes from neutrophils were isolated post-infection and characterized by transmission electron microscopy and flow cytometry. To determine whether these microvesicles influenced mycobactericidal activity, mycobacteria-infected macrophages were treated with isolated ectosomes. RESULTS: Ectosomes were released from neutrophils infected with mycobacteria. These ectosomes were derived from neutrophil plasma membrane and a small proportion stained with PKH26. These microvesicles, when incubated with infected macrophages, influenced antimycobacterial activity. CONCLUSIONS: This is the first study to demonstrate that ectosomes that are shed from infected neutrophils influence mycobactericidal activity in macrophages in vitro, suggesting that these microvesicles have biological significance. Nevertheless, major gaps in our knowledge of microvesicle biology remain.Cellular immune responseTuberculosisHuman polymorphonuclear neutrophilEctosomesMycobacterium tuberculosisMicropartículas Derivadas de Células/imunologiaAtivação de Macrófagos/imunologiaMycobacterium tuberculosis/imunologiaNeutrófilos/imunologiaTuberculose/imunologiaComunicação Celular/imunologiaMicropartículas Derivadas de Células/ultraestruturaCélulas CultivadasCorantes FluorescentesHumanosMacrófagos/imunologiaMicroscopia EletrônicaNeutrófilos/microbiologiaCompostos Orgânicosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8802/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALDuarte TA Mycobacterium....pdfDuarte TA Mycobacterium....pdfapplication/pdf1042368https://www.arca.fiocruz.br/bitstream/icict/8802/2/Duarte%20TA%20Mycobacterium....pdf72a82354af873a14f7ec429585669248MD52TEXTDuarte TA Mycobacterium....pdf.txtDuarte TA Mycobacterium....pdf.txtExtracted texttext/plain40486https://www.arca.fiocruz.br/bitstream/icict/8802/3/Duarte%20TA%20Mycobacterium....pdf.txt4a6ee23e58b6bf28950ef9d06a2ab3baMD53icict/88022023-03-15 14:33:20.763oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:20Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation.
title Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation.
spellingShingle Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation.
Duarte, Tonya Azevedo
Cellular immune response
Tuberculosis
Human polymorphonuclear neutrophil
Ectosomes
Mycobacterium tuberculosis
Micropartículas Derivadas de Células/imunologia
Ativação de Macrófagos/imunologia
Mycobacterium tuberculosis/imunologia
Neutrófilos/imunologia
Tuberculose/imunologia
Comunicação Celular/imunologia
Micropartículas Derivadas de Células/ultraestrutura
Células Cultivadas
Corantes Fluorescentes
Humanos
Macrófagos/imunologia
Microscopia Eletrônica
Neutrófilos/microbiologia
Compostos Orgânicos
title_short Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation.
title_full Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation.
title_fullStr Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation.
title_full_unstemmed Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation.
title_sort Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation.
author Duarte, Tonya Azevedo
author_facet Duarte, Tonya Azevedo
Dutra, Alberto Augusto Noronha
Nery, Joilda Silva
Ribeiro, Samantha Brum
Pitanga, Thassila Nogueira
Silva, José R. Lapa e
Arruda, Sérgio Marcos
Boéchat, Neio
author_role author
author2 Dutra, Alberto Augusto Noronha
Nery, Joilda Silva
Ribeiro, Samantha Brum
Pitanga, Thassila Nogueira
Silva, José R. Lapa e
Arruda, Sérgio Marcos
Boéchat, Neio
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Duarte, Tonya Azevedo
Dutra, Alberto Augusto Noronha
Nery, Joilda Silva
Ribeiro, Samantha Brum
Pitanga, Thassila Nogueira
Silva, José R. Lapa e
Arruda, Sérgio Marcos
Boéchat, Neio
dc.subject.en.pt_BR.fl_str_mv Cellular immune response
Tuberculosis
Human polymorphonuclear neutrophil
Ectosomes
Mycobacterium tuberculosis
topic Cellular immune response
Tuberculosis
Human polymorphonuclear neutrophil
Ectosomes
Mycobacterium tuberculosis
Micropartículas Derivadas de Células/imunologia
Ativação de Macrófagos/imunologia
Mycobacterium tuberculosis/imunologia
Neutrófilos/imunologia
Tuberculose/imunologia
Comunicação Celular/imunologia
Micropartículas Derivadas de Células/ultraestrutura
Células Cultivadas
Corantes Fluorescentes
Humanos
Macrófagos/imunologia
Microscopia Eletrônica
Neutrófilos/microbiologia
Compostos Orgânicos
dc.subject.decs.pt_BR.fl_str_mv Micropartículas Derivadas de Células/imunologia
Ativação de Macrófagos/imunologia
Mycobacterium tuberculosis/imunologia
Neutrófilos/imunologia
Tuberculose/imunologia
Comunicação Celular/imunologia
Micropartículas Derivadas de Células/ultraestrutura
Células Cultivadas
Corantes Fluorescentes
Humanos
Macrófagos/imunologia
Microscopia Eletrônica
Neutrófilos/microbiologia
Compostos Orgânicos
description Federal University of Bahia. Health Science Institute. Salvador, BA, Brasil / Federal University of Rio de Janeiro. Clementino Fraga Filho University Hospital, Department of Internal Medicine. Multidisciplinary Research Laboratory. Rio de Janeiro, RJ, Brasil
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-11-12T14:22:42Z
dc.date.available.fl_str_mv 2014-11-12T14:22:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DUARTE, T. A. et al. Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation. Tuberculosis (Edinb), v. 92, n. 3, p. 218-225, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8802
dc.identifier.issn.none.fl_str_mv 1873-281X
dc.identifier.doi.none.fl_str_mv 10.1016/j.tube.2012.02.007
identifier_str_mv DUARTE, T. A. et al. Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation. Tuberculosis (Edinb), v. 92, n. 3, p. 218-225, 2012.
1873-281X
10.1016/j.tube.2012.02.007
url https://www.arca.fiocruz.br/handle/icict/8802
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier Ltd
publisher.none.fl_str_mv Elsevier Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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collection Repositório Institucional da FIOCRUZ (ARCA)
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