Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation.
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/8802 |
Resumo: | Federal University of Bahia. Health Science Institute. Salvador, BA, Brasil / Federal University of Rio de Janeiro. Clementino Fraga Filho University Hospital, Department of Internal Medicine. Multidisciplinary Research Laboratory. Rio de Janeiro, RJ, Brasil |
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Duarte, Tonya AzevedoDutra, Alberto Augusto NoronhaNery, Joilda SilvaRibeiro, Samantha BrumPitanga, Thassila NogueiraSilva, José R. Lapa eArruda, Sérgio MarcosBoéchat, Neio2014-11-12T14:22:42Z2014-11-12T14:22:42Z2012DUARTE, T. A. et al. Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation. Tuberculosis (Edinb), v. 92, n. 3, p. 218-225, 2012.1873-281Xhttps://www.arca.fiocruz.br/handle/icict/880210.1016/j.tube.2012.02.007engElsevier LtdMycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFederal University of Bahia. Health Science Institute. Salvador, BA, Brasil / Federal University of Rio de Janeiro. Clementino Fraga Filho University Hospital, Department of Internal Medicine. Multidisciplinary Research Laboratory. Rio de Janeiro, RJ, BrasilUniversity College London. Gower Street, London, UK / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFederal University of Rio de Janeiro. Clementino Fraga Filho University Hospital, Department of Internal Medicine. Multidisciplinary Research Laboratory. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFederal University of Rio de Janeiro. Clementino Fraga Filho University Hospital, Department of Internal Medicine. Multidisciplinary Research Laboratory. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFederal University of Rio de Janeiro. Clementino Fraga Filho University Hospital, Department of Internal Medicine. Multidisciplinary Research Laboratory. Rio de Janeiro, RJ, BrasilBACKGROUND: The existence of ectosome-like microvesicles released by neutrophils was proposed a few decades ago. Other studies revealed that the innate immune response during mycobacterial infection is accompanied by an intense migration of neutrophils to the site of infection, which may be important during the acute phase of tuberculosis. We found that the ectosomes derived from infected neutrophils are biologically active and can influence the survival of Mycobacterium tuberculosis within macrophages. METHODS: Mycobacteria were cultured on supplemented Middlebrook-7H9 broth. All strains were grown to the exponential phase and quantitated by serial dilution. Human neutrophils and macrophages were infected with mycobacteria. Ectosomes from neutrophils were isolated post-infection and characterized by transmission electron microscopy and flow cytometry. To determine whether these microvesicles influenced mycobactericidal activity, mycobacteria-infected macrophages were treated with isolated ectosomes. RESULTS: Ectosomes were released from neutrophils infected with mycobacteria. These ectosomes were derived from neutrophil plasma membrane and a small proportion stained with PKH26. These microvesicles, when incubated with infected macrophages, influenced antimycobacterial activity. CONCLUSIONS: This is the first study to demonstrate that ectosomes that are shed from infected neutrophils influence mycobactericidal activity in macrophages in vitro, suggesting that these microvesicles have biological significance. Nevertheless, major gaps in our knowledge of microvesicle biology remain.Cellular immune responseTuberculosisHuman polymorphonuclear neutrophilEctosomesMycobacterium tuberculosisMicropartículas Derivadas de Células/imunologiaAtivação de Macrófagos/imunologiaMycobacterium tuberculosis/imunologiaNeutrófilos/imunologiaTuberculose/imunologiaComunicação Celular/imunologiaMicropartículas Derivadas de Células/ultraestruturaCélulas CultivadasCorantes FluorescentesHumanosMacrófagos/imunologiaMicroscopia EletrônicaNeutrófilos/microbiologiaCompostos Orgânicosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8802/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALDuarte TA Mycobacterium....pdfDuarte TA Mycobacterium....pdfapplication/pdf1042368https://www.arca.fiocruz.br/bitstream/icict/8802/2/Duarte%20TA%20Mycobacterium....pdf72a82354af873a14f7ec429585669248MD52TEXTDuarte TA Mycobacterium....pdf.txtDuarte TA Mycobacterium....pdf.txtExtracted texttext/plain40486https://www.arca.fiocruz.br/bitstream/icict/8802/3/Duarte%20TA%20Mycobacterium....pdf.txt4a6ee23e58b6bf28950ef9d06a2ab3baMD53icict/88022023-03-15 14:33:20.763oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:20Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation. |
title |
Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation. |
spellingShingle |
Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation. Duarte, Tonya Azevedo Cellular immune response Tuberculosis Human polymorphonuclear neutrophil Ectosomes Mycobacterium tuberculosis Micropartículas Derivadas de Células/imunologia Ativação de Macrófagos/imunologia Mycobacterium tuberculosis/imunologia Neutrófilos/imunologia Tuberculose/imunologia Comunicação Celular/imunologia Micropartículas Derivadas de Células/ultraestrutura Células Cultivadas Corantes Fluorescentes Humanos Macrófagos/imunologia Microscopia Eletrônica Neutrófilos/microbiologia Compostos Orgânicos |
title_short |
Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation. |
title_full |
Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation. |
title_fullStr |
Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation. |
title_full_unstemmed |
Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation. |
title_sort |
Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation. |
author |
Duarte, Tonya Azevedo |
author_facet |
Duarte, Tonya Azevedo Dutra, Alberto Augusto Noronha Nery, Joilda Silva Ribeiro, Samantha Brum Pitanga, Thassila Nogueira Silva, José R. Lapa e Arruda, Sérgio Marcos Boéchat, Neio |
author_role |
author |
author2 |
Dutra, Alberto Augusto Noronha Nery, Joilda Silva Ribeiro, Samantha Brum Pitanga, Thassila Nogueira Silva, José R. Lapa e Arruda, Sérgio Marcos Boéchat, Neio |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Duarte, Tonya Azevedo Dutra, Alberto Augusto Noronha Nery, Joilda Silva Ribeiro, Samantha Brum Pitanga, Thassila Nogueira Silva, José R. Lapa e Arruda, Sérgio Marcos Boéchat, Neio |
dc.subject.en.pt_BR.fl_str_mv |
Cellular immune response Tuberculosis Human polymorphonuclear neutrophil Ectosomes Mycobacterium tuberculosis |
topic |
Cellular immune response Tuberculosis Human polymorphonuclear neutrophil Ectosomes Mycobacterium tuberculosis Micropartículas Derivadas de Células/imunologia Ativação de Macrófagos/imunologia Mycobacterium tuberculosis/imunologia Neutrófilos/imunologia Tuberculose/imunologia Comunicação Celular/imunologia Micropartículas Derivadas de Células/ultraestrutura Células Cultivadas Corantes Fluorescentes Humanos Macrófagos/imunologia Microscopia Eletrônica Neutrófilos/microbiologia Compostos Orgânicos |
dc.subject.decs.pt_BR.fl_str_mv |
Micropartículas Derivadas de Células/imunologia Ativação de Macrófagos/imunologia Mycobacterium tuberculosis/imunologia Neutrófilos/imunologia Tuberculose/imunologia Comunicação Celular/imunologia Micropartículas Derivadas de Células/ultraestrutura Células Cultivadas Corantes Fluorescentes Humanos Macrófagos/imunologia Microscopia Eletrônica Neutrófilos/microbiologia Compostos Orgânicos |
description |
Federal University of Bahia. Health Science Institute. Salvador, BA, Brasil / Federal University of Rio de Janeiro. Clementino Fraga Filho University Hospital, Department of Internal Medicine. Multidisciplinary Research Laboratory. Rio de Janeiro, RJ, Brasil |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2014-11-12T14:22:42Z |
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2014-11-12T14:22:42Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
DUARTE, T. A. et al. Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation. Tuberculosis (Edinb), v. 92, n. 3, p. 218-225, 2012. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/8802 |
dc.identifier.issn.none.fl_str_mv |
1873-281X |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.tube.2012.02.007 |
identifier_str_mv |
DUARTE, T. A. et al. Mycobacterium tuberculosis-induced neutrophil ectosomes decrease macrophage activation. Tuberculosis (Edinb), v. 92, n. 3, p. 218-225, 2012. 1873-281X 10.1016/j.tube.2012.02.007 |
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https://www.arca.fiocruz.br/handle/icict/8802 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Elsevier Ltd |
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Elsevier Ltd |
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