TcI/TcII co-infection can enhance Trypanosoma cruzi growth in Rhodnius prolixus

Detalhes bibliográficos
Autor(a) principal: Araújo, Catarina A. C
Data de Publicação: 2014
Outros Autores: Waniek, Peter J, Jansen, Ana Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10093
Resumo: We thank Prof. J. Jurberg (Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, Fiocruz, Rio de Janeiro) for providing the insects and Prof. Dr. V. Bongertz (Laboratório de AIDS e Imunologia Molecular, Fiocruz, Rio de Janeiro) for the English corrections. This work was supported by Fundação de Amparo à Pesquisa no Estado do Rio de Janeiro (FAPERJ: E-26/ 100.456/2007), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq: Edital Universal 472276/2006-9; PDJ: 152069/2006-2) and Fundação Oswaldo Cruz (FIOCRUZ). CACA was a CNPq Research Fellow (151187/2009-6) and PJW was a FAPERJ Research Fellow (E-26/152.913/2005).
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spelling Araújo, Catarina A. CWaniek, Peter JJansen, Ana Maria2015-04-22T14:23:07Z2015-04-22T14:23:07Z2014ARAÚJO, Catarina A; WANIEK, Peter J.; JANSEN, Ana M. TcI/TcII co-infection can enhance Trypanosoma cruzi growth in Rhodnius prolixus. Parasites & Vectors, v.7, n.94, 8p, 2014.1756-3305https://www.arca.fiocruz.br/handle/icict/1009310.1186/1756-3305-7-94We thank Prof. J. Jurberg (Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, Fiocruz, Rio de Janeiro) for providing the insects and Prof. Dr. V. Bongertz (Laboratório de AIDS e Imunologia Molecular, Fiocruz, Rio de Janeiro) for the English corrections. This work was supported by Fundação de Amparo à Pesquisa no Estado do Rio de Janeiro (FAPERJ: E-26/ 100.456/2007), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq: Edital Universal 472276/2006-9; PDJ: 152069/2006-2) and Fundação Oswaldo Cruz (FIOCRUZ). CACA was a CNPq Research Fellow (151187/2009-6) and PJW was a FAPERJ Research Fellow (E-26/152.913/2005).Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, BrasilBackground: Rhodnius prolixus is an obligate haematophagous insect and one of the most important vectors of Trypanosoma cruzi, the causative agent of Chagas disease in the Americas. T. cruzi is a highly variable parasite which is not transmitted in the same efficiency by the different triatomine vectors. Because different T. cruzi genotypes are aetiopathologically divergent, further elucidation of the transmission abilities of different Chagas disease vectors is extremely important. Findings: In the present study, the growth behaviour of two T. cruzi isolates, MDID/BR/1993/C45 (TcI) and TBRA/BR/ 1999/JCA3 (TcII), sharing the same microhabitat (intestinal tract) in single and mixed infections, was examined. The distribution patterns and parasite population densities were evaluated at 7, 14 and 21 days after feeding (daf) by quantification of parasites using Neubauer haemocytometric measurements and mini-exon PCR to identify TcI and TcII subpopulations. Parasitic colonization in the small intestine was more successful in the mixed infection model than the single infection models at 21 daf. In the rectal lumen and wall, the growth behaviour of the mixed infection was similar to that of the TcI group, although the total parasite number was lower. In the TcII group, no metacyclic trypomastigote forms were found. PCR analysis of the contents of each dissected region showed different genotype fractions in the mixed infection model, in which TcI seemed to be the predominant isolate. Conclusion: The different growth behaviour of the TcI and TcII isolates in single and mixed infection models demonstrated that possibly an intraspecific factor modulates parasitic development in the intestine of R. prolixus.engBioMed CentralTrypanosomaTrato GastrointestinalDoença de ChagasRhodniusTrypanosoma cruziTclTdlMixed InfectionsRhodnius prolixusDigestive tractChagas DiseaseTracto GastrointestinalTrypanosomaEnfermedad de ChagasRhodniusRhodniusTrato GastrointestinalTcI/TcII co-infection can enhance Trypanosoma cruzi growth in Rhodnius prolixusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10093/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALcatarina_araujoetal_IOC_2014.pdfapplication/pdf362757https://www.arca.fiocruz.br/bitstream/icict/10093/2/catarina_araujoetal_IOC_2014.pdfa8aeb5f677f8cc4d71789993e7736fc9MD52TEXTcatarina_araujoetal_IOC_2014.pdf.txtcatarina_araujoetal_IOC_2014.pdf.txtExtracted texttext/plain39615https://www.arca.fiocruz.br/bitstream/icict/10093/3/catarina_araujoetal_IOC_2014.pdf.txt835ad1cb4012fc8507b134ff7fb478d7MD53icict/100932022-06-24 12:17:35.221oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:17:35Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv TcI/TcII co-infection can enhance Trypanosoma cruzi growth in Rhodnius prolixus
title TcI/TcII co-infection can enhance Trypanosoma cruzi growth in Rhodnius prolixus
spellingShingle TcI/TcII co-infection can enhance Trypanosoma cruzi growth in Rhodnius prolixus
Araújo, Catarina A. C
Trypanosoma
Trato Gastrointestinal
Doença de Chagas
Rhodnius
Trypanosoma cruzi
Tcl
Tdl
Mixed Infections
Rhodnius prolixus
Digestive tract
Chagas Disease
Tracto Gastrointestinal
Trypanosoma
Enfermedad de Chagas
Rhodnius
Rhodnius
Trato Gastrointestinal
title_short TcI/TcII co-infection can enhance Trypanosoma cruzi growth in Rhodnius prolixus
title_full TcI/TcII co-infection can enhance Trypanosoma cruzi growth in Rhodnius prolixus
title_fullStr TcI/TcII co-infection can enhance Trypanosoma cruzi growth in Rhodnius prolixus
title_full_unstemmed TcI/TcII co-infection can enhance Trypanosoma cruzi growth in Rhodnius prolixus
title_sort TcI/TcII co-infection can enhance Trypanosoma cruzi growth in Rhodnius prolixus
author Araújo, Catarina A. C
author_facet Araújo, Catarina A. C
Waniek, Peter J
Jansen, Ana Maria
author_role author
author2 Waniek, Peter J
Jansen, Ana Maria
author2_role author
author
dc.contributor.author.fl_str_mv Araújo, Catarina A. C
Waniek, Peter J
Jansen, Ana Maria
dc.subject.other.pt_BR.fl_str_mv Trypanosoma
Trato Gastrointestinal
Doença de Chagas
Rhodnius
topic Trypanosoma
Trato Gastrointestinal
Doença de Chagas
Rhodnius
Trypanosoma cruzi
Tcl
Tdl
Mixed Infections
Rhodnius prolixus
Digestive tract
Chagas Disease
Tracto Gastrointestinal
Trypanosoma
Enfermedad de Chagas
Rhodnius
Rhodnius
Trato Gastrointestinal
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Tcl
Tdl
Mixed Infections
Rhodnius prolixus
Digestive tract
Chagas Disease
dc.subject.es.pt_BR.fl_str_mv Tracto Gastrointestinal
Trypanosoma
Enfermedad de Chagas
Rhodnius
dc.subject.decs.pt_BR.fl_str_mv Rhodnius
Trato Gastrointestinal
description We thank Prof. J. Jurberg (Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, Fiocruz, Rio de Janeiro) for providing the insects and Prof. Dr. V. Bongertz (Laboratório de AIDS e Imunologia Molecular, Fiocruz, Rio de Janeiro) for the English corrections. This work was supported by Fundação de Amparo à Pesquisa no Estado do Rio de Janeiro (FAPERJ: E-26/ 100.456/2007), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq: Edital Universal 472276/2006-9; PDJ: 152069/2006-2) and Fundação Oswaldo Cruz (FIOCRUZ). CACA was a CNPq Research Fellow (151187/2009-6) and PJW was a FAPERJ Research Fellow (E-26/152.913/2005).
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-04-22T14:23:07Z
dc.date.available.fl_str_mv 2015-04-22T14:23:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv ARAÚJO, Catarina A; WANIEK, Peter J.; JANSEN, Ana M. TcI/TcII co-infection can enhance Trypanosoma cruzi growth in Rhodnius prolixus. Parasites & Vectors, v.7, n.94, 8p, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10093
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.pt_BR.fl_str_mv 10.1186/1756-3305-7-94
identifier_str_mv ARAÚJO, Catarina A; WANIEK, Peter J.; JANSEN, Ana M. TcI/TcII co-infection can enhance Trypanosoma cruzi growth in Rhodnius prolixus. Parasites & Vectors, v.7, n.94, 8p, 2014.
1756-3305
10.1186/1756-3305-7-94
url https://www.arca.fiocruz.br/handle/icict/10093
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv BioMed Central
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