A proteomic approach to identify proteins from Trichuris trichiura extract with immunomodulatory effects.

Detalhes bibliográficos
Autor(a) principal: Santos, Leonardo Nascimento
Data de Publicação: 2013
Outros Autores: Gaallo, Margareth Borges Coutinho, Silva, Eduardo Santos, Figueiredo, Camila Alexandrina Viana, Cooper, Philip John, Barreto, Maurício Lima, Loureiro, S, Pontes-de-Carvalho, Lain Carlos, Neves, Neuza Maria Alcântara
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8223
Resumo: Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeração. Salvador, BA, Brasil
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spelling Santos, Leonardo NascimentoGaallo, Margareth Borges CoutinhoSilva, Eduardo SantosFigueiredo, Camila Alexandrina VianaCooper, Philip JohnBarreto, Maurício LimaLoureiro, SPontes-de-Carvalho, Lain CarlosNeves, Neuza Maria Alcântara2014-08-25T16:31:26Z2014-08-25T16:31:26Z2013SANTOS, L. N. et al. A proteomic approach to identify proteins from Trichuris trichiura extract with immunomodulatory effects. Parasite Immunology, v. 35, n. 5-6, p. 188-193, 2013.1365-3024https://www.arca.fiocruz.br/handle/icict/822310.1111/pim.12025engJohn Wiley & Sons, LtdA proteomic approach to identify proteins from Trichuris trichiura extract with immunomodulatory effects.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeração. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Plataforma de Espectrometria de Massas. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeração. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeração. Salvador, BA, BrasilLiverpool School of Tropical Medicine. Department of Molecular and Biochemical Parasitology. Liverpool, UKUniversidade Federal da Bahia. Instituto de Saúde Coletiva. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Saúde Coletiva. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laborat orio de Patologia e Biointervenção. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeração. Salvador, BA, BrasilInfections with Trichuris trichiura and other trichurid nematodes have been reported to display protective effects against atopy, allergic and autoimmune diseases. The aims of the present study were to investigate the immunomodulatory properties of T. trichiura adult worm extract (TtE) and its fractions (TtEFs) on the production of cytokines by peripheral blood mononuclear cells and to identify their proteinaceous components. Fourteen TtEFs were obtained by ion exchange chromatography and tested for effects on cytokine production by peripheral blood mononuclear cells. The molecular constituents of the six most active fractions were evaluated using nano-LC/mass spectrometry. The homology between T. trichiura and the related nematode Trichinella spiralis was used to identify 12 proteins in TtEFs. Among those identified, fructose biphosphate aldolase, a homologue of macrophage migration inhibitory factor and heat-shock protein 70 may contribute to the immunomodulatory effects of TtEFs. The identification of such proteins could lead to the development of novel drugs for the therapy of allergic and other inflammatory diseasesCytokineImmunoregulationPeripheral blood mononuclear cellsProteomicsTrichuris sppCitocinas/sangueProteínas de Helminto/imunologiaLeucócitos Mononucleares/imunologiaTrichuris/imunologiaAdultoAnimaisCriançaCromatografia por Troca IônicaFrutose-Bifosfato Aldolase/químicaProteínas de Choque Térmico HSP70/imunologiaProteínas de Helminto/químicaHumanosProteômicaTrichinella spiralis/químicaTricuríase/imunologiaAdulto Joveminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8223/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALSANTOS. L.N A proteomic approach to identify.....pdfSANTOS. 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dc.title.pt_BR.fl_str_mv A proteomic approach to identify proteins from Trichuris trichiura extract with immunomodulatory effects.
title A proteomic approach to identify proteins from Trichuris trichiura extract with immunomodulatory effects.
spellingShingle A proteomic approach to identify proteins from Trichuris trichiura extract with immunomodulatory effects.
Santos, Leonardo Nascimento
Cytokine
Immunoregulation
Peripheral blood mononuclear cells
Proteomics
Trichuris spp
Citocinas/sangue
Proteínas de Helminto/imunologia
Leucócitos Mononucleares/imunologia
Trichuris/imunologia
Adulto
Animais
Criança
Cromatografia por Troca Iônica
Frutose-Bifosfato Aldolase/química
Proteínas de Choque Térmico HSP70/imunologia
Proteínas de Helminto/química
Humanos
Proteômica
Trichinella spiralis/química
Tricuríase/imunologia
Adulto Jovem
title_short A proteomic approach to identify proteins from Trichuris trichiura extract with immunomodulatory effects.
title_full A proteomic approach to identify proteins from Trichuris trichiura extract with immunomodulatory effects.
title_fullStr A proteomic approach to identify proteins from Trichuris trichiura extract with immunomodulatory effects.
title_full_unstemmed A proteomic approach to identify proteins from Trichuris trichiura extract with immunomodulatory effects.
title_sort A proteomic approach to identify proteins from Trichuris trichiura extract with immunomodulatory effects.
author Santos, Leonardo Nascimento
author_facet Santos, Leonardo Nascimento
Gaallo, Margareth Borges Coutinho
Silva, Eduardo Santos
Figueiredo, Camila Alexandrina Viana
Cooper, Philip John
Barreto, Maurício Lima
Loureiro, S
Pontes-de-Carvalho, Lain Carlos
Neves, Neuza Maria Alcântara
author_role author
author2 Gaallo, Margareth Borges Coutinho
Silva, Eduardo Santos
Figueiredo, Camila Alexandrina Viana
Cooper, Philip John
Barreto, Maurício Lima
Loureiro, S
Pontes-de-Carvalho, Lain Carlos
Neves, Neuza Maria Alcântara
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Leonardo Nascimento
Gaallo, Margareth Borges Coutinho
Silva, Eduardo Santos
Figueiredo, Camila Alexandrina Viana
Cooper, Philip John
Barreto, Maurício Lima
Loureiro, S
Pontes-de-Carvalho, Lain Carlos
Neves, Neuza Maria Alcântara
dc.subject.en.pt_BR.fl_str_mv Cytokine
Immunoregulation
Peripheral blood mononuclear cells
Proteomics
Trichuris spp
topic Cytokine
Immunoregulation
Peripheral blood mononuclear cells
Proteomics
Trichuris spp
Citocinas/sangue
Proteínas de Helminto/imunologia
Leucócitos Mononucleares/imunologia
Trichuris/imunologia
Adulto
Animais
Criança
Cromatografia por Troca Iônica
Frutose-Bifosfato Aldolase/química
Proteínas de Choque Térmico HSP70/imunologia
Proteínas de Helminto/química
Humanos
Proteômica
Trichinella spiralis/química
Tricuríase/imunologia
Adulto Jovem
dc.subject.decs.pt_BR.fl_str_mv Citocinas/sangue
Proteínas de Helminto/imunologia
Leucócitos Mononucleares/imunologia
Trichuris/imunologia
Adulto
Animais
Criança
Cromatografia por Troca Iônica
Frutose-Bifosfato Aldolase/química
Proteínas de Choque Térmico HSP70/imunologia
Proteínas de Helminto/química
Humanos
Proteômica
Trichinella spiralis/química
Tricuríase/imunologia
Adulto Jovem
description Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Ciências da Biointeração. Salvador, BA, Brasil
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-08-25T16:31:26Z
dc.date.available.fl_str_mv 2014-08-25T16:31:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, L. N. et al. A proteomic approach to identify proteins from Trichuris trichiura extract with immunomodulatory effects. Parasite Immunology, v. 35, n. 5-6, p. 188-193, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8223
dc.identifier.issn.none.fl_str_mv 1365-3024
dc.identifier.doi.none.fl_str_mv 10.1111/pim.12025
identifier_str_mv SANTOS, L. N. et al. A proteomic approach to identify proteins from Trichuris trichiura extract with immunomodulatory effects. Parasite Immunology, v. 35, n. 5-6, p. 188-193, 2013.
1365-3024
10.1111/pim.12025
url https://www.arca.fiocruz.br/handle/icict/8223
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons, Ltd
publisher.none.fl_str_mv John Wiley & Sons, Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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