Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules

Detalhes bibliográficos
Autor(a) principal: Shigunov, Patrícia
Data de Publicação: 2014
Outros Autores: Sotelo-Silveira, Jose, Stimamiglio, Marco Augusto, Kuligovski, Crisciele, Irigoín, Florencia, Badano, Jose L, Munroe, David J., Dominguez, Alejandro Correa, Dallagiovanna, Bruno
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8972
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
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spelling Shigunov, PatríciaSotelo-Silveira, JoseStimamiglio, Marco AugustoKuligovski, CriscieleIrigoín, FlorenciaBadano, Jose LMunroe, David J.Dominguez, Alejandro CorreaDallagiovanna, Bruno2014-11-26T13:11:26Z2014-11-26T13:11:26Z2014SHIGUNOV, Patrícia et al. Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules. BMC Molecular Biology, v.15, n. 12, p. 1-14, 2014.1471-2199https://www.arca.fiocruz.br/handle/icict/897210.1186/1471-2199-15-12porBioMed CentralRibonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granulesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Instituto de Investigaciones Biológicas Clemente Estable. Genomics Department. Montevideo, Uruguay. / Universidad de la República Uruguay. Facultad de Ciencias. Departamento de Biología Celular y Molecular. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Instituto Pasteur. Montevideo, Uruguay. / Universidad de la República Uruguay. Facultad de Ciencias. Departamento de Biología Celular y Molecular. Montevideo, Uruguay.Instituto Pasteur. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.National Laboratory for Cancer Research. Leidos Biomedical Research, Inc. Cancer Research Technology Program. Frederick, USA.Background: DZIP1 (DAZ-interacting protein 1) has been described as a component of the Hh signaling pathway with a putative regulatory role in ciliogenesis. DZIP1 interacts with DAZ RNA binding proteins in embryonic stem cells and human germ cells suggesting a role in mRNA regulation. Results: We investigated DZIP1 function in HeLa cells and its involvement in ribonucleoprotein complexes. DZIP1 was predominantly located in granules in the cytoplasm. Under oxidative stress conditions, DZIP1 re-localized to stress granules. DZIP appears to be important for the formation of stress granules during the stress response. We used immunoprecipitation assays with antibodies against DZIP1 and microarray hybridization to identify mRNAs associated with DZIP1. The genetic networks formed by the DZIP1-associated mRNAs were involved in cell cycle and gene expression regulation. DZIP1 is involved in the Hedgehog signaling pathway. We used cyclopamine, a specific inhibitor of this pathway, to analyze the expression of DZIP1 and its associated mRNAs. The abundance of DZIP1-associated mRNAs increased with treatment; however, the silencing or overexpression of DZIP1 in HeLa cells had no effect on the accumulation of the associated mRNAs. Polysomal profile analysis by sucrose gradient centrifugation demonstrated the presence of DZIP1 in the polysomal fraction. Conclusions: Our results suggest that DZIP1 is part of an RNP complex that occupies various subcellular locations. The diversity of the mRNAs associated with DZIP1 suggests that this protein is a component of different RNPs associated with translating polysomes and with RNA granules.DZIP1RibonucleoproteinStress granulesPolysomeHedgehog signalingRibonucleoproteínasPolirribossomosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALartigo 1.pdfartigo 1.pdfapplication/pdf1716335https://www.arca.fiocruz.br/bitstream/icict/8972/2/artigo%201.pdf17480039c8f2b4b9a96d1131ed066e83MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8972/3/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD53TEXTartigo 1.pdf.txtartigo 1.pdf.txtExtracted texttext/plain61796https://www.arca.fiocruz.br/bitstream/icict/8972/4/artigo%201.pdf.txtd4ebbe5c4e05f95aca2c128239440f2eMD54icict/89722019-11-23 20:10:37.455oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-11-23T23:10:37Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules
title Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules
spellingShingle Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules
Shigunov, Patrícia
DZIP1
Ribonucleoprotein
Stress granules
Polysome
Hedgehog signaling
Ribonucleoproteínas
Polirribossomos
title_short Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules
title_full Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules
title_fullStr Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules
title_full_unstemmed Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules
title_sort Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules
author Shigunov, Patrícia
author_facet Shigunov, Patrícia
Sotelo-Silveira, Jose
Stimamiglio, Marco Augusto
Kuligovski, Crisciele
Irigoín, Florencia
Badano, Jose L
Munroe, David J.
Dominguez, Alejandro Correa
Dallagiovanna, Bruno
author_role author
author2 Sotelo-Silveira, Jose
Stimamiglio, Marco Augusto
Kuligovski, Crisciele
Irigoín, Florencia
Badano, Jose L
Munroe, David J.
Dominguez, Alejandro Correa
Dallagiovanna, Bruno
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Shigunov, Patrícia
Sotelo-Silveira, Jose
Stimamiglio, Marco Augusto
Kuligovski, Crisciele
Irigoín, Florencia
Badano, Jose L
Munroe, David J.
Dominguez, Alejandro Correa
Dallagiovanna, Bruno
dc.subject.en.pt_BR.fl_str_mv DZIP1
Ribonucleoprotein
Stress granules
Polysome
Hedgehog signaling
topic DZIP1
Ribonucleoprotein
Stress granules
Polysome
Hedgehog signaling
Ribonucleoproteínas
Polirribossomos
dc.subject.decs.pt_BR.fl_str_mv Ribonucleoproteínas
Polirribossomos
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
publishDate 2014
dc.date.accessioned.fl_str_mv 2014-11-26T13:11:26Z
dc.date.available.fl_str_mv 2014-11-26T13:11:26Z
dc.date.issued.fl_str_mv 2014
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv SHIGUNOV, Patrícia et al. Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules. BMC Molecular Biology, v.15, n. 12, p. 1-14, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8972
dc.identifier.issn.pt_BR.fl_str_mv 1471-2199
dc.identifier.doi.none.fl_str_mv 10.1186/1471-2199-15-12
identifier_str_mv SHIGUNOV, Patrícia et al. Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules. BMC Molecular Biology, v.15, n. 12, p. 1-14, 2014.
1471-2199
10.1186/1471-2199-15-12
url https://www.arca.fiocruz.br/handle/icict/8972
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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