Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/8972 |
Resumo: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. |
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Shigunov, PatríciaSotelo-Silveira, JoseStimamiglio, Marco AugustoKuligovski, CriscieleIrigoín, FlorenciaBadano, Jose LMunroe, David J.Dominguez, Alejandro CorreaDallagiovanna, Bruno2014-11-26T13:11:26Z2014-11-26T13:11:26Z2014SHIGUNOV, Patrícia et al. Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules. BMC Molecular Biology, v.15, n. 12, p. 1-14, 2014.1471-2199https://www.arca.fiocruz.br/handle/icict/897210.1186/1471-2199-15-12porBioMed CentralRibonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granulesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Instituto de Investigaciones Biológicas Clemente Estable. Genomics Department. Montevideo, Uruguay. / Universidad de la República Uruguay. Facultad de Ciencias. Departamento de Biología Celular y Molecular. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Instituto Pasteur. Montevideo, Uruguay. / Universidad de la República Uruguay. Facultad de Ciencias. Departamento de Biología Celular y Molecular. Montevideo, Uruguay.Instituto Pasteur. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.National Laboratory for Cancer Research. Leidos Biomedical Research, Inc. Cancer Research Technology Program. Frederick, USA.Background: DZIP1 (DAZ-interacting protein 1) has been described as a component of the Hh signaling pathway with a putative regulatory role in ciliogenesis. DZIP1 interacts with DAZ RNA binding proteins in embryonic stem cells and human germ cells suggesting a role in mRNA regulation. Results: We investigated DZIP1 function in HeLa cells and its involvement in ribonucleoprotein complexes. DZIP1 was predominantly located in granules in the cytoplasm. Under oxidative stress conditions, DZIP1 re-localized to stress granules. DZIP appears to be important for the formation of stress granules during the stress response. We used immunoprecipitation assays with antibodies against DZIP1 and microarray hybridization to identify mRNAs associated with DZIP1. The genetic networks formed by the DZIP1-associated mRNAs were involved in cell cycle and gene expression regulation. DZIP1 is involved in the Hedgehog signaling pathway. We used cyclopamine, a specific inhibitor of this pathway, to analyze the expression of DZIP1 and its associated mRNAs. The abundance of DZIP1-associated mRNAs increased with treatment; however, the silencing or overexpression of DZIP1 in HeLa cells had no effect on the accumulation of the associated mRNAs. Polysomal profile analysis by sucrose gradient centrifugation demonstrated the presence of DZIP1 in the polysomal fraction. Conclusions: Our results suggest that DZIP1 is part of an RNP complex that occupies various subcellular locations. The diversity of the mRNAs associated with DZIP1 suggests that this protein is a component of different RNPs associated with translating polysomes and with RNA granules.DZIP1RibonucleoproteinStress granulesPolysomeHedgehog signalingRibonucleoproteínasPolirribossomosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALartigo 1.pdfartigo 1.pdfapplication/pdf1716335https://www.arca.fiocruz.br/bitstream/icict/8972/2/artigo%201.pdf17480039c8f2b4b9a96d1131ed066e83MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8972/3/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD53TEXTartigo 1.pdf.txtartigo 1.pdf.txtExtracted texttext/plain61796https://www.arca.fiocruz.br/bitstream/icict/8972/4/artigo%201.pdf.txtd4ebbe5c4e05f95aca2c128239440f2eMD54icict/89722019-11-23 20:10:37.455oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-11-23T23:10:37Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules |
title |
Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules |
spellingShingle |
Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules Shigunov, Patrícia DZIP1 Ribonucleoprotein Stress granules Polysome Hedgehog signaling Ribonucleoproteínas Polirribossomos |
title_short |
Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules |
title_full |
Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules |
title_fullStr |
Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules |
title_full_unstemmed |
Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules |
title_sort |
Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules |
author |
Shigunov, Patrícia |
author_facet |
Shigunov, Patrícia Sotelo-Silveira, Jose Stimamiglio, Marco Augusto Kuligovski, Crisciele Irigoín, Florencia Badano, Jose L Munroe, David J. Dominguez, Alejandro Correa Dallagiovanna, Bruno |
author_role |
author |
author2 |
Sotelo-Silveira, Jose Stimamiglio, Marco Augusto Kuligovski, Crisciele Irigoín, Florencia Badano, Jose L Munroe, David J. Dominguez, Alejandro Correa Dallagiovanna, Bruno |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Shigunov, Patrícia Sotelo-Silveira, Jose Stimamiglio, Marco Augusto Kuligovski, Crisciele Irigoín, Florencia Badano, Jose L Munroe, David J. Dominguez, Alejandro Correa Dallagiovanna, Bruno |
dc.subject.en.pt_BR.fl_str_mv |
DZIP1 Ribonucleoprotein Stress granules Polysome Hedgehog signaling |
topic |
DZIP1 Ribonucleoprotein Stress granules Polysome Hedgehog signaling Ribonucleoproteínas Polirribossomos |
dc.subject.decs.pt_BR.fl_str_mv |
Ribonucleoproteínas Polirribossomos |
description |
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. |
publishDate |
2014 |
dc.date.accessioned.fl_str_mv |
2014-11-26T13:11:26Z |
dc.date.available.fl_str_mv |
2014-11-26T13:11:26Z |
dc.date.issued.fl_str_mv |
2014 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
SHIGUNOV, Patrícia et al. Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules. BMC Molecular Biology, v.15, n. 12, p. 1-14, 2014. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/8972 |
dc.identifier.issn.pt_BR.fl_str_mv |
1471-2199 |
dc.identifier.doi.none.fl_str_mv |
10.1186/1471-2199-15-12 |
identifier_str_mv |
SHIGUNOV, Patrícia et al. Ribonomic analysis of human DZIP1 reveals its involvement in ribonucleoprotein complexes and stress granules. BMC Molecular Biology, v.15, n. 12, p. 1-14, 2014. 1471-2199 10.1186/1471-2199-15-12 |
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BioMed Central |
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BioMed Central |
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