Inhibition of HIV-1 replication in human primary cells by a Dolabellane Diterpene isolated from the marine Algae Dictyota pfaffii

Detalhes bibliográficos
Autor(a) principal: Santos, Claudio Cesar Cirne
Data de Publicação: 2006
Outros Autores: Teixeira, Valéria L., Castello-Branco, Luiz Roberto, Frugulhetti, Izabel C. P. P., Bou-Habib, Dumith Chequer
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1055/s-2005-916209
Texto Completo: https://www.arca.fiocruz.br/handle/icict/38957
Resumo: Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Laboratório de Virologia Molecular. Niterói, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil.
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spelling Santos, Claudio Cesar CirneTeixeira, Valéria L.Castello-Branco, Luiz RobertoFrugulhetti, Izabel C. P. P.Bou-Habib, Dumith Chequer2020-01-03T13:29:06Z2020-01-03T13:29:06Z2006SANTOS, Claudio Cesar Cirne et al. Inhibition of HIV-1 replication in human primary cells by a Dolabellane Diterpene isolated from the marine Algae Dictyota pfaffii. Planta Medica, v. 72, p. 295-299, 2006.0032-0943https://www.arca.fiocruz.br/handle/icict/3895710.1055/s-2005-9162091439-0221engGeorg Thieme VerlagHIV-1AIDSDictyota pfaffiiDictyota pfaffiiHIV-1AIDSDiterpenesDolabellaneInhibition of HIV-1 replication in human primary cells by a Dolabellane Diterpene isolated from the marine Algae Dictyota pfaffiiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Laboratório de Virologia Molecular. Niterói, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Departamento de Biologia Marinha. Laboratório de Biologia Marinha. Niteói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Laboratório de Virologia Molecular. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil.We describe in this paper that the dolabellane diterpene 8,10,18-trihydroxy-2,6-dolabelladiene (3), isolated from the marine algae Dictyota pfaffii, inhibits the HIV-1 infection in human primary cells and tumor cell lines. We initially observed that compound 3 inhibited the activity of a purified HIV-1 enzyme reverse transcriptase (RT) in a dose-dependent manner, with an IC (50) value of 16.5 +/- 4.3 microM. Next, we found that compound 3 inhibited HIV-1 infection by an R5-tropic isolate in peripheral blood mononuclear cells (PBMCs) in a dose-dependent manner with an EC (50) value of 8.4 +/- 2.8 microM. The replication of HIV-1 isolates presenting distinct tropism for chemokine receptors was also inhibited, as analyzed in PBMCs or U87 cells infected with R5-, X4- or R5X4-tropic isolates. Likewise, compound 3 blocked HIV-1 infection in macrophages by R5 and R5X4 viruses in a dose-dependent manner with EC (50) values of 1.7 +/- 0.6 microM and 1.85 +/- 0.75 microM, respectively. Compound 3 sustained antiretroviral activity even when added to HIV-1-infected Sup-T1 cells at 12 h after infection, suggesting that, as well as inhibiting HIV-1 RT, it also blocks HIV-1 replication at a post transcriptional step. Our results support further investigations on compound 3 pharmacokinetics and we propose that this diterpene could be considered as a potential compound for HIV-1 therapy.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/38957/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLuizRR_CasteloBranco_etal_IOC_2006.pdfLuizRR_CasteloBranco_etal_IOC_2006.pdfapplication/pdf88033https://www.arca.fiocruz.br/bitstream/icict/38957/2/LuizRR_CasteloBranco_etal_IOC_2006.pdf1c07f1773a945cdb2a8eece147efc60aMD52TEXTLuizRR_CasteloBranco_etal_IOC_2006.pdf.txtLuizRR_CasteloBranco_etal_IOC_2006.pdf.txtExtracted texttext/plain16681https://www.arca.fiocruz.br/bitstream/icict/38957/3/LuizRR_CasteloBranco_etal_IOC_2006.pdf.txtc28855ec6258a5996bf8ce90c1565188MD53icict/389572020-01-06 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dc.title.pt_BR.fl_str_mv Inhibition of HIV-1 replication in human primary cells by a Dolabellane Diterpene isolated from the marine Algae Dictyota pfaffii
title Inhibition of HIV-1 replication in human primary cells by a Dolabellane Diterpene isolated from the marine Algae Dictyota pfaffii
spellingShingle Inhibition of HIV-1 replication in human primary cells by a Dolabellane Diterpene isolated from the marine Algae Dictyota pfaffii
Santos, Claudio Cesar Cirne
HIV-1
AIDS
Dictyota pfaffii
Dictyota pfaffii
HIV-1
AIDS
Diterpenes
Dolabellane
title_short Inhibition of HIV-1 replication in human primary cells by a Dolabellane Diterpene isolated from the marine Algae Dictyota pfaffii
title_full Inhibition of HIV-1 replication in human primary cells by a Dolabellane Diterpene isolated from the marine Algae Dictyota pfaffii
title_fullStr Inhibition of HIV-1 replication in human primary cells by a Dolabellane Diterpene isolated from the marine Algae Dictyota pfaffii
title_full_unstemmed Inhibition of HIV-1 replication in human primary cells by a Dolabellane Diterpene isolated from the marine Algae Dictyota pfaffii
title_sort Inhibition of HIV-1 replication in human primary cells by a Dolabellane Diterpene isolated from the marine Algae Dictyota pfaffii
author Santos, Claudio Cesar Cirne
author_facet Santos, Claudio Cesar Cirne
Teixeira, Valéria L.
Castello-Branco, Luiz Roberto
Frugulhetti, Izabel C. P. P.
Bou-Habib, Dumith Chequer
author_role author
author2 Teixeira, Valéria L.
Castello-Branco, Luiz Roberto
Frugulhetti, Izabel C. P. P.
Bou-Habib, Dumith Chequer
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Santos, Claudio Cesar Cirne
Teixeira, Valéria L.
Castello-Branco, Luiz Roberto
Frugulhetti, Izabel C. P. P.
Bou-Habib, Dumith Chequer
dc.subject.other.pt_BR.fl_str_mv HIV-1
AIDS
Dictyota pfaffii
topic HIV-1
AIDS
Dictyota pfaffii
Dictyota pfaffii
HIV-1
AIDS
Diterpenes
Dolabellane
dc.subject.en.pt_BR.fl_str_mv Dictyota pfaffii
HIV-1
AIDS
Diterpenes
Dolabellane
description Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Laboratório de Virologia Molecular. Niterói, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ, Brasil.
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2020-01-03T13:29:06Z
dc.date.available.fl_str_mv 2020-01-03T13:29:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Claudio Cesar Cirne et al. Inhibition of HIV-1 replication in human primary cells by a Dolabellane Diterpene isolated from the marine Algae Dictyota pfaffii. Planta Medica, v. 72, p. 295-299, 2006.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/38957
dc.identifier.issn.pt_BR.fl_str_mv 0032-0943
dc.identifier.doi.none.fl_str_mv 10.1055/s-2005-916209
dc.identifier.eissn.none.fl_str_mv 1439-0221
identifier_str_mv SANTOS, Claudio Cesar Cirne et al. Inhibition of HIV-1 replication in human primary cells by a Dolabellane Diterpene isolated from the marine Algae Dictyota pfaffii. Planta Medica, v. 72, p. 295-299, 2006.
0032-0943
10.1055/s-2005-916209
1439-0221
url https://www.arca.fiocruz.br/handle/icict/38957
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Georg Thieme Verlag
publisher.none.fl_str_mv Georg Thieme Verlag
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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