Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-alpha secretion and reduce parasite replication in macrophages.

Detalhes bibliográficos
Autor(a) principal: Montalvão, Fabricio
Data de Publicação: 2010
Outros Autores: Almeida, Geisy Monteiro, Silva, Elisabeth Martins da, Borges, Valeria de Matos, Vasconcellos, Rita de Cássia dos Santos, Takiya, Christina Maeda, Lopes, Marcela de Freitas, Nunes, Marise Pinheiro, DosReis, George Alexandre
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9464
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil
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spelling Montalvão, FabricioAlmeida, Geisy MonteiroSilva, Elisabeth Martins daBorges, Valeria de MatosVasconcellos, Rita de Cássia dos SantosTakiya, Christina MaedaLopes, Marcela de FreitasNunes, Marise PinheiroDosReis, George Alexandre2015-02-09T17:40:06Z2015-02-09T17:40:06Z2010MONTALVÃO, F. et al. Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-alpha secretion and reduce parasite replication in macrophages. European Journal of Immunology, v. 40, n. 2, p. 417-425, 2010.1521-4141https://www.arca.fiocruz.br/handle/icict/946410.1002/eji.200939606engWiley-VCH Verlag GmbH & Co. KGaAApoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-alpha secretion and reduce parasite replication in macrophages.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, BrasilUniversidade Federal Fluminense. Instituto Biomédico. Niterói, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal Fluminense. Instituto de Biologia. Niterói, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Saúde e Ambiente na Região Amazônica. Conselho Nacional de Desenvolvimento Científico e Tecnológico. Amazonas, MA, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Saúde e Ambiente na Região Amazônica. Conselho Nacional de Desenvolvimento Científico e Tecnológico. Amazonas, MA, BrasilPhagocytic removal of apoptotic lymphocytes exacerbates replication of Trypanosoma cruzi in macrophages. We investigated the presence of Ab against apoptotic lymphocytes in T. cruzi infection and the role of these Ab in parasite replication. Both control and chagasic serum contained IgG Ab that opsonized apoptotic lymphocytes. Treatment of apoptotic lymphocytes with purified IgG from chagasic, but not control serum, reduced T. cruzi replication in macrophages. The protective effect of chagasic IgG depended on Fcgamma receptors, as demonstrated by the requirement for the intact Fc portion of IgG, and the effect could be abrogated by treating macrophages with an anti-CD16/CD32 Fab fragment. Chagasic IgG displayed increased reactivity against a subset of apoptotic cell Ag, as measured by flow cytometry and immunoblot analyses. Apoptotic lymphocytes treated with chagasic IgG, but not control IgG, increased production of TNF-alpha, while decreasing production of TGF-beta1 by infected macrophages. Increased control of parasite replication required TNF-alpha production. Previous immunization with apoptotic cells or injection of apoptotic cells opsonized with chagasic IgG reduced parasitemia in infected mice. These results indicate that Ab raised against apoptotic cells could play a protective role in control of T. cruzi replication by macrophages.ApoptosisFc receptorsPhagocytosisTrypanosoma cruziAnticorpos Antiprotozoários/imunologiaDoença de Chagas/imunologiaLinfócitos/imunologiaMacrófagos/imunologiaTrypanosoma cruzi/imunologiaFator de Necrose Tumoral alfa/secreçãoTransferência AdotivaAnimaisAnticorpos Antiprotozoários/farmacologiaApoptoseCélulas CultivadasDoença de Chagas/parasitologiaTécnicas de CoculturaCitometria de FluxoImunoglobulina G/imunologiaLinfócitos/citologiaMacrófagos/citologiaMasculinoCamundongosParasitemia/imunologiaFator de Crescimento Transformador beta1/metabolismoTrypanosoma cruzi/efeitos de drogasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9464/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALMontalvao F Apoptotic...pdfMontalvao F Apoptotic...pdfapplication/pdf863295https://www.arca.fiocruz.br/bitstream/icict/9464/2/Montalvao%20F%20Apoptotic...pdfd03a35f95c14528a92900489c246a968MD52TEXTMontalvao F Apoptotic...pdf.txtMontalvao F Apoptotic...pdf.txtExtracted texttext/plain44944https://www.arca.fiocruz.br/bitstream/icict/9464/3/Montalvao%20F%20Apoptotic...pdf.txta1e9a66bfe00bc410423055d70e7f63eMD53icict/94642023-03-15 14:33:04.409oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:04Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-alpha secretion and reduce parasite replication in macrophages.
title Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-alpha secretion and reduce parasite replication in macrophages.
spellingShingle Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-alpha secretion and reduce parasite replication in macrophages.
Montalvão, Fabricio
Apoptosis
Fc receptors
Phagocytosis
Trypanosoma cruzi
Anticorpos Antiprotozoários/imunologia
Doença de Chagas/imunologia
Linfócitos/imunologia
Macrófagos/imunologia
Trypanosoma cruzi/imunologia
Fator de Necrose Tumoral alfa/secreção
Transferência Adotiva
Animais
Anticorpos Antiprotozoários/farmacologia
Apoptose
Células Cultivadas
Doença de Chagas/parasitologia
Técnicas de Cocultura
Citometria de Fluxo
Imunoglobulina G/imunologia
Linfócitos/citologia
Macrófagos/citologia
Masculino
Camundongos
Parasitemia/imunologia
Fator de Crescimento Transformador beta1/metabolismo
Trypanosoma cruzi/efeitos de drogas
title_short Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-alpha secretion and reduce parasite replication in macrophages.
title_full Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-alpha secretion and reduce parasite replication in macrophages.
title_fullStr Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-alpha secretion and reduce parasite replication in macrophages.
title_full_unstemmed Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-alpha secretion and reduce parasite replication in macrophages.
title_sort Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-alpha secretion and reduce parasite replication in macrophages.
author Montalvão, Fabricio
author_facet Montalvão, Fabricio
Almeida, Geisy Monteiro
Silva, Elisabeth Martins da
Borges, Valeria de Matos
Vasconcellos, Rita de Cássia dos Santos
Takiya, Christina Maeda
Lopes, Marcela de Freitas
Nunes, Marise Pinheiro
DosReis, George Alexandre
author_role author
author2 Almeida, Geisy Monteiro
Silva, Elisabeth Martins da
Borges, Valeria de Matos
Vasconcellos, Rita de Cássia dos Santos
Takiya, Christina Maeda
Lopes, Marcela de Freitas
Nunes, Marise Pinheiro
DosReis, George Alexandre
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Montalvão, Fabricio
Almeida, Geisy Monteiro
Silva, Elisabeth Martins da
Borges, Valeria de Matos
Vasconcellos, Rita de Cássia dos Santos
Takiya, Christina Maeda
Lopes, Marcela de Freitas
Nunes, Marise Pinheiro
DosReis, George Alexandre
dc.subject.en.pt_BR.fl_str_mv Apoptosis
Fc receptors
Phagocytosis
Trypanosoma cruzi
topic Apoptosis
Fc receptors
Phagocytosis
Trypanosoma cruzi
Anticorpos Antiprotozoários/imunologia
Doença de Chagas/imunologia
Linfócitos/imunologia
Macrófagos/imunologia
Trypanosoma cruzi/imunologia
Fator de Necrose Tumoral alfa/secreção
Transferência Adotiva
Animais
Anticorpos Antiprotozoários/farmacologia
Apoptose
Células Cultivadas
Doença de Chagas/parasitologia
Técnicas de Cocultura
Citometria de Fluxo
Imunoglobulina G/imunologia
Linfócitos/citologia
Macrófagos/citologia
Masculino
Camundongos
Parasitemia/imunologia
Fator de Crescimento Transformador beta1/metabolismo
Trypanosoma cruzi/efeitos de drogas
dc.subject.decs.pt_BR.fl_str_mv Anticorpos Antiprotozoários/imunologia
Doença de Chagas/imunologia
Linfócitos/imunologia
Macrófagos/imunologia
Trypanosoma cruzi/imunologia
Fator de Necrose Tumoral alfa/secreção
Transferência Adotiva
Animais
Anticorpos Antiprotozoários/farmacologia
Apoptose
Células Cultivadas
Doença de Chagas/parasitologia
Técnicas de Cocultura
Citometria de Fluxo
Imunoglobulina G/imunologia
Linfócitos/citologia
Macrófagos/citologia
Masculino
Camundongos
Parasitemia/imunologia
Fator de Crescimento Transformador beta1/metabolismo
Trypanosoma cruzi/efeitos de drogas
description Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2015-02-09T17:40:06Z
dc.date.available.fl_str_mv 2015-02-09T17:40:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MONTALVÃO, F. et al. Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-alpha secretion and reduce parasite replication in macrophages. European Journal of Immunology, v. 40, n. 2, p. 417-425, 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9464
dc.identifier.issn.none.fl_str_mv 1521-4141
dc.identifier.doi.none.fl_str_mv 10.1002/eji.200939606
identifier_str_mv MONTALVÃO, F. et al. Apoptotic lymphocytes treated with IgG from Trypanosoma cruzi infection increase TNF-alpha secretion and reduce parasite replication in macrophages. European Journal of Immunology, v. 40, n. 2, p. 417-425, 2010.
1521-4141
10.1002/eji.200939606
url https://www.arca.fiocruz.br/handle/icict/9464
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley-VCH Verlag GmbH & Co. KGaA
publisher.none.fl_str_mv Wiley-VCH Verlag GmbH & Co. KGaA
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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