Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis

Bibliographic Details
Main Author: Fonseca, Fernanda Lopes
Publication Date: 2013
Other Authors: Guimarães, Allan Jefferson, Kmetzsch, Lívia, Dutra, Fabianno F., Silva, Fernanda D., Taborda, Carlos P., Araujo, Glauber de S., Frases, Susana, Staats, Charley C., Bozza, Marcelo T., Schrank, Augusto, Vainstein, Marilene Henning, Nimrichter, Leonardo, Casadevall, Arturo, Rodrigues, Marcio Lourenço
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
Download full: https://www.arca.fiocruz.br/handle/icict/31982
Summary: Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.
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spelling Fonseca, Fernanda LopesGuimarães, Allan JeffersonKmetzsch, LíviaDutra, Fabianno F.Silva, Fernanda D.Taborda, Carlos P.Araujo, Glauber de S.Frases, SusanaStaats, Charley C.Bozza, Marcelo T.Schrank, AugustoVainstein, Marilene HenningNimrichter, LeonardoCasadevall, ArturoRodrigues, Marcio Lourenço2019-03-07T17:29:54Z2019-03-07T17:29:54Z2013FONSECA, Fernanda L. et al. Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis. Fungal Geneticsand Biology, v. 60, p. 64-73, 2013.1087-1845https://www.arca.fiocruz.br/handle/icict/3198210.1016/j.fgb.2013.04.005engElsevierBinding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia, Imunologia e Parasitologia. Niterói, RJ, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Departamento de Biologia Molecular e Biotecnologia. Porto Alegre, RS, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. Instituto de Ciências Biomédicas. São Paulo, SP, Brasil.Universidade de São Paulo. Instituto de Ciências Biomédicas. São Paulo, SP, Brasil.Instituto Nacional de Metrologia, Normalização e Qualidade Industrial. Laboratório de Biologia. Rio de Janeiro, RJ, Brasil / Univerdade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Metrologia, Normalização e Qualidade Industrial. Laboratório de Biologia. Rio de Janeiro, RJ, Brasil / Univerdade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Departamento de Biologia Molecular e Biotecnologia. Porto Alegre, RS, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Departamento de Biologia Molecular e Biotecnologia. Porto Alegre, RS, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Departamento de Biologia Molecular e Biotecnologia. Porto Alegre, RS, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.Albert Einstein College of Medicine. Department of Microbiology and Immunology. Division of Infection Diseases of the Department of Medicine. Bronx, NY, USA.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.The principal capsular component of Cryptococcus neoformans, glucuronoxylomannan (GXM), interacts with surface glycans, including chitin-like oligomers. Although the role of GXM in cryptococcal infection has been well explored, there is no information on how chitooligomers affect fungal pathogenesis. In this study, surface chitooligomers of C. neoformans were blocked through the use of the wheat germ lectin (WGA) and the effects on animal pathogenesis, interaction with host cells, fungal growth and capsule formation were analyzed. Treatment of C. neoformans cells with WGA followed by infection of mice delayed mortality relative to animals infected with untreated fungal cells. This observation was associated with reduced brain colonization by lectin-treated cryptococci. Blocking chitooligomers also rendered yeast cells less efficient in their ability to associate with phagocytes. WGA did not affect fungal viability, but inhibited GXM release to the extracellular space and capsule formation. In WGA-treated yeast cells, genes that are involved in capsule formation and GXM traffic had their transcription levels decreased in comparison with untreated cells. Our results suggest that cellular pathways required for capsule formation and pathogenic mechanisms are affected by blocking chitin-derived structures at the cell surface of C. neoformans. Targeting chitooligomers with specific ligands may reveal new therapeutic alternatives to control cryptococcosis.Cryptococcus neoformansVirulenceLectin bindingCapsuleChitin oligosaccharidesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83101https://www.arca.fiocruz.br/bitstream/icict/31982/1/license.txt425ef259bcb460998407fbfed7c102d0MD51ORIGINALve_Marcio_Rodrigues_etal_CDTS_2013b.pdfve_Marcio_Rodrigues_etal_CDTS_2013b.pdfapplication/pdf1866304https://www.arca.fiocruz.br/bitstream/icict/31982/2/ve_Marcio_Rodrigues_etal_CDTS_2013b.pdf82f4cd152b95e064141a84c9b69577d0MD52TEXTve_Marcio_Rodrigues_etal_CDTS_2013b.pdf.txtve_Marcio_Rodrigues_etal_CDTS_2013b.pdf.txtExtracted texttext/plain56756https://www.arca.fiocruz.br/bitstream/icict/31982/3/ve_Marcio_Rodrigues_etal_CDTS_2013b.pdf.txtf57eccf75d53331dbdd883b9b0aecbcbMD53icict/319822023-02-23 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dc.title.pt_BR.fl_str_mv Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis
title Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis
spellingShingle Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis
Fonseca, Fernanda Lopes
Cryptococcus neoformans
Virulence
Lectin binding
Capsule
Chitin oligosaccharides
title_short Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis
title_full Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis
title_fullStr Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis
title_full_unstemmed Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis
title_sort Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis
author Fonseca, Fernanda Lopes
author_facet Fonseca, Fernanda Lopes
Guimarães, Allan Jefferson
Kmetzsch, Lívia
Dutra, Fabianno F.
Silva, Fernanda D.
Taborda, Carlos P.
Araujo, Glauber de S.
Frases, Susana
Staats, Charley C.
Bozza, Marcelo T.
Schrank, Augusto
Vainstein, Marilene Henning
Nimrichter, Leonardo
Casadevall, Arturo
Rodrigues, Marcio Lourenço
author_role author
author2 Guimarães, Allan Jefferson
Kmetzsch, Lívia
Dutra, Fabianno F.
Silva, Fernanda D.
Taborda, Carlos P.
Araujo, Glauber de S.
Frases, Susana
Staats, Charley C.
Bozza, Marcelo T.
Schrank, Augusto
Vainstein, Marilene Henning
Nimrichter, Leonardo
Casadevall, Arturo
Rodrigues, Marcio Lourenço
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fonseca, Fernanda Lopes
Guimarães, Allan Jefferson
Kmetzsch, Lívia
Dutra, Fabianno F.
Silva, Fernanda D.
Taborda, Carlos P.
Araujo, Glauber de S.
Frases, Susana
Staats, Charley C.
Bozza, Marcelo T.
Schrank, Augusto
Vainstein, Marilene Henning
Nimrichter, Leonardo
Casadevall, Arturo
Rodrigues, Marcio Lourenço
dc.subject.en.pt_BR.fl_str_mv Cryptococcus neoformans
Virulence
Lectin binding
Capsule
Chitin oligosaccharides
topic Cryptococcus neoformans
Virulence
Lectin binding
Capsule
Chitin oligosaccharides
description Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2019-03-07T17:29:54Z
dc.date.available.fl_str_mv 2019-03-07T17:29:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FONSECA, Fernanda L. et al. Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis. Fungal Geneticsand Biology, v. 60, p. 64-73, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31982
dc.identifier.issn.pt_BR.fl_str_mv 1087-1845
dc.identifier.doi.none.fl_str_mv 10.1016/j.fgb.2013.04.005
identifier_str_mv FONSECA, Fernanda L. et al. Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis. Fungal Geneticsand Biology, v. 60, p. 64-73, 2013.
1087-1845
10.1016/j.fgb.2013.04.005
url https://www.arca.fiocruz.br/handle/icict/31982
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/31982/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/31982/2/ve_Marcio_Rodrigues_etal_CDTS_2013b.pdf
https://www.arca.fiocruz.br/bitstream/icict/31982/3/ve_Marcio_Rodrigues_etal_CDTS_2013b.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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