Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1016/j.fgb.2013.04.005 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/31982 |
Resumo: | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil. |
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Fonseca, Fernanda LopesGuimarães, Allan JeffersonKmetzsch, LíviaDutra, Fabianno F.Silva, Fernanda D.Taborda, Carlos P.Araujo, Glauber de S.Frases, SusanaStaats, Charley C.Bozza, Marcelo T.Schrank, AugustoVainstein, Marilene HenningNimrichter, LeonardoCasadevall, ArturoRodrigues, Marcio Lourenço2019-03-07T17:29:54Z2019-03-07T17:29:54Z2013FONSECA, Fernanda L. et al. Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis. Fungal Geneticsand Biology, v. 60, p. 64-73, 2013.1087-1845https://www.arca.fiocruz.br/handle/icict/3198210.1016/j.fgb.2013.04.005engElsevierBinding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia, Imunologia e Parasitologia. Niterói, RJ, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Departamento de Biologia Molecular e Biotecnologia. Porto Alegre, RS, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. Instituto de Ciências Biomédicas. São Paulo, SP, Brasil.Universidade de São Paulo. Instituto de Ciências Biomédicas. São Paulo, SP, Brasil.Instituto Nacional de Metrologia, Normalização e Qualidade Industrial. Laboratório de Biologia. Rio de Janeiro, RJ, Brasil / Univerdade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Metrologia, Normalização e Qualidade Industrial. Laboratório de Biologia. Rio de Janeiro, RJ, Brasil / Univerdade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Departamento de Biologia Molecular e Biotecnologia. Porto Alegre, RS, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Departamento de Biologia Molecular e Biotecnologia. Porto Alegre, RS, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Departamento de Biologia Molecular e Biotecnologia. Porto Alegre, RS, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.Albert Einstein College of Medicine. Department of Microbiology and Immunology. Division of Infection Diseases of the Department of Medicine. Bronx, NY, USA.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.The principal capsular component of Cryptococcus neoformans, glucuronoxylomannan (GXM), interacts with surface glycans, including chitin-like oligomers. Although the role of GXM in cryptococcal infection has been well explored, there is no information on how chitooligomers affect fungal pathogenesis. In this study, surface chitooligomers of C. neoformans were blocked through the use of the wheat germ lectin (WGA) and the effects on animal pathogenesis, interaction with host cells, fungal growth and capsule formation were analyzed. Treatment of C. neoformans cells with WGA followed by infection of mice delayed mortality relative to animals infected with untreated fungal cells. This observation was associated with reduced brain colonization by lectin-treated cryptococci. Blocking chitooligomers also rendered yeast cells less efficient in their ability to associate with phagocytes. WGA did not affect fungal viability, but inhibited GXM release to the extracellular space and capsule formation. In WGA-treated yeast cells, genes that are involved in capsule formation and GXM traffic had their transcription levels decreased in comparison with untreated cells. Our results suggest that cellular pathways required for capsule formation and pathogenic mechanisms are affected by blocking chitin-derived structures at the cell surface of C. neoformans. Targeting chitooligomers with specific ligands may reveal new therapeutic alternatives to control cryptococcosis.Cryptococcus neoformansVirulenceLectin bindingCapsuleChitin oligosaccharidesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83101https://www.arca.fiocruz.br/bitstream/icict/31982/1/license.txt425ef259bcb460998407fbfed7c102d0MD51ORIGINALve_Marcio_Rodrigues_etal_CDTS_2013b.pdfve_Marcio_Rodrigues_etal_CDTS_2013b.pdfapplication/pdf1866304https://www.arca.fiocruz.br/bitstream/icict/31982/2/ve_Marcio_Rodrigues_etal_CDTS_2013b.pdf82f4cd152b95e064141a84c9b69577d0MD52TEXTve_Marcio_Rodrigues_etal_CDTS_2013b.pdf.txtve_Marcio_Rodrigues_etal_CDTS_2013b.pdf.txtExtracted texttext/plain56756https://www.arca.fiocruz.br/bitstream/icict/31982/3/ve_Marcio_Rodrigues_etal_CDTS_2013b.pdf.txtf57eccf75d53331dbdd883b9b0aecbcbMD53icict/319822023-02-23 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| dc.title.pt_BR.fl_str_mv |
Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis |
| title |
Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis |
| spellingShingle |
Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis Fonseca, Fernanda Lopes Cryptococcus neoformans Virulence Lectin binding Capsule Chitin oligosaccharides |
| title_short |
Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis |
| title_full |
Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis |
| title_fullStr |
Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis |
| title_full_unstemmed |
Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis |
| title_sort |
Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis |
| author |
Fonseca, Fernanda Lopes |
| author_facet |
Fonseca, Fernanda Lopes Guimarães, Allan Jefferson Kmetzsch, Lívia Dutra, Fabianno F. Silva, Fernanda D. Taborda, Carlos P. Araujo, Glauber de S. Frases, Susana Staats, Charley C. Bozza, Marcelo T. Schrank, Augusto Vainstein, Marilene Henning Nimrichter, Leonardo Casadevall, Arturo Rodrigues, Marcio Lourenço |
| author_role |
author |
| author2 |
Guimarães, Allan Jefferson Kmetzsch, Lívia Dutra, Fabianno F. Silva, Fernanda D. Taborda, Carlos P. Araujo, Glauber de S. Frases, Susana Staats, Charley C. Bozza, Marcelo T. Schrank, Augusto Vainstein, Marilene Henning Nimrichter, Leonardo Casadevall, Arturo Rodrigues, Marcio Lourenço |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Fonseca, Fernanda Lopes Guimarães, Allan Jefferson Kmetzsch, Lívia Dutra, Fabianno F. Silva, Fernanda D. Taborda, Carlos P. Araujo, Glauber de S. Frases, Susana Staats, Charley C. Bozza, Marcelo T. Schrank, Augusto Vainstein, Marilene Henning Nimrichter, Leonardo Casadevall, Arturo Rodrigues, Marcio Lourenço |
| dc.subject.en.pt_BR.fl_str_mv |
Cryptococcus neoformans Virulence Lectin binding Capsule Chitin oligosaccharides |
| topic |
Cryptococcus neoformans Virulence Lectin binding Capsule Chitin oligosaccharides |
| description |
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil. |
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2013 |
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2013 |
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2019-03-07T17:29:54Z |
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2019-03-07T17:29:54Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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FONSECA, Fernanda L. et al. Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis. Fungal Geneticsand Biology, v. 60, p. 64-73, 2013. |
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https://www.arca.fiocruz.br/handle/icict/31982 |
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1087-1845 |
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10.1016/j.fgb.2013.04.005 |
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FONSECA, Fernanda L. et al. Binding of the wheat germ lectin to Cryptococcus neoformans chitooligomers affects multiple mechanisms required for fungal pathogenesis. Fungal Geneticsand Biology, v. 60, p. 64-73, 2013. 1087-1845 10.1016/j.fgb.2013.04.005 |
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