IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro.
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7813 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Integrado de Patologia e Biointervenção. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / UFMS-MS. Depto. de Morfofisiologia. Salvador, BA, Brasil |
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Rodrigues, Cleusa Alves TheodoroBatista, Luís Fábio da SilvaTeixeira Filho, Roberto SantosSantos, Claire da SilvaPinheiro, Cristiane Garboggini Melo deAlmeida, Taís Fontoura deFreitas, Luiz Antonio Rodrigues de2014-06-11T17:23:43Z2014-06-11T17:23:43Z2009RODRIGUES, C. A. T. et al. IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro. Veterinary Immunology and Immunopathology, v. 127, n. 3-4, p. 382-388, 2009.0165-2427https://www.arca.fiocruz.br/handle/icict/781310.1016/j.vetimm.2008.10.324engElsevier B.VIFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Integrado de Patologia e Biointervenção. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / UFMS-MS. Depto. de Morfofisiologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Integrado de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Integrado de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imunoparasitologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Integrado de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Integrado de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Integrado de Patologia e Biointervenção. Salvador, BA, Brasil / UFBA. Faculdade de Medicina. Salvador, BA, BrasilWhile the response to Leishmania spp. is well characterized in mice and humans, much less is known concerning the canine immune response, particularly soon after exposure to the parasite. Early events are considered to be a determinant of infection outcome. To investigate the dog’s early immune response to L. chagasi, an in vitro priming system (PIV) using dog naı¨ve PBMC was established. Until now, dog PIV immune response to L. chagasi has not been assessed. We co-cultivated PBMC primarily stimulated with L. chagasi in vitro with autologous infected macrophages and found that IFN-g mRNA is up-regulated in these cells compared to control unstimulated cells. IL-4 and IL-10 mRNA expression by L. chagasi-stimulated PBMC was similar to control unstimulated PBMCwhen incubated with infected macrophages. Surprisingly, correlation studies showed that a lower IFN-g/IL-4 expression ratio correlated with a lower percentage of infection. We propose that the direct correlation between IFN-g/IL-4 ratio and parasite load is dependent on the higher correlation of both IFN-g and IL-4 expression with lower parasite infection. This PIV system was shown to be useful in evaluating the dog immune response to L. chagasi, and results indicate that a balance between IFN-g and IL-4 is associated with control of parasite infection in vitro.Leishmania chagasiPrimary in vitro systemIFN-yDoenças do Cão/imunologiaInterferon gama/metabolismoLeishmania/imunologiaLeucócitos Mononucleares/metabolismoLeucócitos Mononucleares/parasitologiaRegulação para Cima/imunologiaCélulas CultivadasAnimaisCãesInterferon gama/genéticaMacrófagos/metabolismoMacrófagos/parasitologiaMasculinoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALRodrigues CAT IFN-y expression....pdfRodrigues CAT IFN-y expression....pdfapplication/pdf284804https://www.arca.fiocruz.br/bitstream/icict/7813/1/Rodrigues%20CAT%20IFN-y%20expression....pdf8deea07db1b090684bd9aeacd243dab0MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7813/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTRodrigues CAT IFN-y expression....pdf.txtRodrigues CAT IFN-y expression....pdf.txtExtracted texttext/plain36717https://www.arca.fiocruz.br/bitstream/icict/7813/5/Rodrigues%20CAT%20IFN-y%20expression....pdf.txtade2d988cbcf4db41935917a694d2769MD55THUMBNAILRodrigues CAT IFN-y expression....pdf.jpgRodrigues CAT IFN-y expression....pdf.jpgGenerated Thumbnailimage/jpeg2088https://www.arca.fiocruz.br/bitstream/icict/7813/4/Rodrigues%20CAT%20IFN-y%20expression....pdf.jpgfe7fd520ae554158c0b5e1f35e866b26MD54icict/78132023-03-15 14:33:22.881oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:22Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro. |
title |
IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro. |
spellingShingle |
IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro. Rodrigues, Cleusa Alves Theodoro Leishmania chagasi Primary in vitro system IFN-y Doenças do Cão/imunologia Interferon gama/metabolismo Leishmania/imunologia Leucócitos Mononucleares/metabolismo Leucócitos Mononucleares/parasitologia Regulação para Cima/imunologia Células Cultivadas Animais Cães Interferon gama/genética Macrófagos/metabolismo Macrófagos/parasitologia Masculino |
title_short |
IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro. |
title_full |
IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro. |
title_fullStr |
IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro. |
title_full_unstemmed |
IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro. |
title_sort |
IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro. |
author |
Rodrigues, Cleusa Alves Theodoro |
author_facet |
Rodrigues, Cleusa Alves Theodoro Batista, Luís Fábio da Silva Teixeira Filho, Roberto Santos Santos, Claire da Silva Pinheiro, Cristiane Garboggini Melo de Almeida, Taís Fontoura de Freitas, Luiz Antonio Rodrigues de |
author_role |
author |
author2 |
Batista, Luís Fábio da Silva Teixeira Filho, Roberto Santos Santos, Claire da Silva Pinheiro, Cristiane Garboggini Melo de Almeida, Taís Fontoura de Freitas, Luiz Antonio Rodrigues de |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues, Cleusa Alves Theodoro Batista, Luís Fábio da Silva Teixeira Filho, Roberto Santos Santos, Claire da Silva Pinheiro, Cristiane Garboggini Melo de Almeida, Taís Fontoura de Freitas, Luiz Antonio Rodrigues de |
dc.subject.en.pt_BR.fl_str_mv |
Leishmania chagasi Primary in vitro system IFN-y |
topic |
Leishmania chagasi Primary in vitro system IFN-y Doenças do Cão/imunologia Interferon gama/metabolismo Leishmania/imunologia Leucócitos Mononucleares/metabolismo Leucócitos Mononucleares/parasitologia Regulação para Cima/imunologia Células Cultivadas Animais Cães Interferon gama/genética Macrófagos/metabolismo Macrófagos/parasitologia Masculino |
dc.subject.decs.pt_BR.fl_str_mv |
Doenças do Cão/imunologia Interferon gama/metabolismo Leishmania/imunologia Leucócitos Mononucleares/metabolismo Leucócitos Mononucleares/parasitologia Regulação para Cima/imunologia Células Cultivadas Animais Cães Interferon gama/genética Macrófagos/metabolismo Macrófagos/parasitologia Masculino |
description |
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Integrado de Patologia e Biointervenção. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / UFMS-MS. Depto. de Morfofisiologia. Salvador, BA, Brasil |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009 |
dc.date.accessioned.fl_str_mv |
2014-06-11T17:23:43Z |
dc.date.available.fl_str_mv |
2014-06-11T17:23:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RODRIGUES, C. A. T. et al. IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro. Veterinary Immunology and Immunopathology, v. 127, n. 3-4, p. 382-388, 2009. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/7813 |
dc.identifier.issn.none.fl_str_mv |
0165-2427 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.vetimm.2008.10.324 |
identifier_str_mv |
RODRIGUES, C. A. T. et al. IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro. Veterinary Immunology and Immunopathology, v. 127, n. 3-4, p. 382-388, 2009. 0165-2427 10.1016/j.vetimm.2008.10.324 |
url |
https://www.arca.fiocruz.br/handle/icict/7813 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Elsevier B.V |
publisher.none.fl_str_mv |
Elsevier B.V |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Fundação Oswaldo Cruz (FIOCRUZ) |
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FIOCRUZ |
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FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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Repositório Institucional da FIOCRUZ (ARCA) |
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