IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro.

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Cleusa Alves Theodoro
Data de Publicação: 2009
Outros Autores: Batista, Luís Fábio da Silva, Teixeira Filho, Roberto Santos, Santos, Claire da Silva, Pinheiro, Cristiane Garboggini Melo de, Almeida, Taís Fontoura de, Freitas, Luiz Antonio Rodrigues de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7813
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Integrado de Patologia e Biointervenção. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / UFMS-MS. Depto. de Morfofisiologia. Salvador, BA, Brasil
id CRUZ_ca68778f6d4e3bab56188b2fdf1e50b3
oai_identifier_str oai:www.arca.fiocruz.br:icict/7813
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Rodrigues, Cleusa Alves TheodoroBatista, Luís Fábio da SilvaTeixeira Filho, Roberto SantosSantos, Claire da SilvaPinheiro, Cristiane Garboggini Melo deAlmeida, Taís Fontoura deFreitas, Luiz Antonio Rodrigues de2014-06-11T17:23:43Z2014-06-11T17:23:43Z2009RODRIGUES, C. A. T. et al. IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro. Veterinary Immunology and Immunopathology, v. 127, n. 3-4, p. 382-388, 2009.0165-2427https://www.arca.fiocruz.br/handle/icict/781310.1016/j.vetimm.2008.10.324engElsevier B.VIFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Integrado de Patologia e Biointervenção. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / UFMS-MS. Depto. de Morfofisiologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Integrado de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Integrado de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imunoparasitologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Integrado de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Integrado de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Integrado de Patologia e Biointervenção. Salvador, BA, Brasil / UFBA. Faculdade de Medicina. Salvador, BA, BrasilWhile the response to Leishmania spp. is well characterized in mice and humans, much less is known concerning the canine immune response, particularly soon after exposure to the parasite. Early events are considered to be a determinant of infection outcome. To investigate the dog’s early immune response to L. chagasi, an in vitro priming system (PIV) using dog naı¨ve PBMC was established. Until now, dog PIV immune response to L. chagasi has not been assessed. We co-cultivated PBMC primarily stimulated with L. chagasi in vitro with autologous infected macrophages and found that IFN-g mRNA is up-regulated in these cells compared to control unstimulated cells. IL-4 and IL-10 mRNA expression by L. chagasi-stimulated PBMC was similar to control unstimulated PBMCwhen incubated with infected macrophages. Surprisingly, correlation studies showed that a lower IFN-g/IL-4 expression ratio correlated with a lower percentage of infection. We propose that the direct correlation between IFN-g/IL-4 ratio and parasite load is dependent on the higher correlation of both IFN-g and IL-4 expression with lower parasite infection. This PIV system was shown to be useful in evaluating the dog immune response to L. chagasi, and results indicate that a balance between IFN-g and IL-4 is associated with control of parasite infection in vitro.Leishmania chagasiPrimary in vitro systemIFN-yDoenças do Cão/imunologiaInterferon gama/metabolismoLeishmania/imunologiaLeucócitos Mononucleares/metabolismoLeucócitos Mononucleares/parasitologiaRegulação para Cima/imunologiaCélulas CultivadasAnimaisCãesInterferon gama/genéticaMacrófagos/metabolismoMacrófagos/parasitologiaMasculinoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALRodrigues CAT IFN-y expression....pdfRodrigues CAT IFN-y expression....pdfapplication/pdf284804https://www.arca.fiocruz.br/bitstream/icict/7813/1/Rodrigues%20CAT%20IFN-y%20expression....pdf8deea07db1b090684bd9aeacd243dab0MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7813/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTRodrigues CAT IFN-y expression....pdf.txtRodrigues CAT IFN-y expression....pdf.txtExtracted texttext/plain36717https://www.arca.fiocruz.br/bitstream/icict/7813/5/Rodrigues%20CAT%20IFN-y%20expression....pdf.txtade2d988cbcf4db41935917a694d2769MD55THUMBNAILRodrigues CAT IFN-y expression....pdf.jpgRodrigues CAT IFN-y expression....pdf.jpgGenerated Thumbnailimage/jpeg2088https://www.arca.fiocruz.br/bitstream/icict/7813/4/Rodrigues%20CAT%20IFN-y%20expression....pdf.jpgfe7fd520ae554158c0b5e1f35e866b26MD54icict/78132023-03-15 14:33:22.881oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:22Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro.
title IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro.
spellingShingle IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro.
Rodrigues, Cleusa Alves Theodoro
Leishmania chagasi
Primary in vitro system
IFN-y
Doenças do Cão/imunologia
Interferon gama/metabolismo
Leishmania/imunologia
Leucócitos Mononucleares/metabolismo
Leucócitos Mononucleares/parasitologia
Regulação para Cima/imunologia
Células Cultivadas
Animais
Cães
Interferon gama/genética
Macrófagos/metabolismo
Macrófagos/parasitologia
Masculino
title_short IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro.
title_full IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro.
title_fullStr IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro.
title_full_unstemmed IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro.
title_sort IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro.
author Rodrigues, Cleusa Alves Theodoro
author_facet Rodrigues, Cleusa Alves Theodoro
Batista, Luís Fábio da Silva
Teixeira Filho, Roberto Santos
Santos, Claire da Silva
Pinheiro, Cristiane Garboggini Melo de
Almeida, Taís Fontoura de
Freitas, Luiz Antonio Rodrigues de
author_role author
author2 Batista, Luís Fábio da Silva
Teixeira Filho, Roberto Santos
Santos, Claire da Silva
Pinheiro, Cristiane Garboggini Melo de
Almeida, Taís Fontoura de
Freitas, Luiz Antonio Rodrigues de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rodrigues, Cleusa Alves Theodoro
Batista, Luís Fábio da Silva
Teixeira Filho, Roberto Santos
Santos, Claire da Silva
Pinheiro, Cristiane Garboggini Melo de
Almeida, Taís Fontoura de
Freitas, Luiz Antonio Rodrigues de
dc.subject.en.pt_BR.fl_str_mv Leishmania chagasi
Primary in vitro system
IFN-y
topic Leishmania chagasi
Primary in vitro system
IFN-y
Doenças do Cão/imunologia
Interferon gama/metabolismo
Leishmania/imunologia
Leucócitos Mononucleares/metabolismo
Leucócitos Mononucleares/parasitologia
Regulação para Cima/imunologia
Células Cultivadas
Animais
Cães
Interferon gama/genética
Macrófagos/metabolismo
Macrófagos/parasitologia
Masculino
dc.subject.decs.pt_BR.fl_str_mv Doenças do Cão/imunologia
Interferon gama/metabolismo
Leishmania/imunologia
Leucócitos Mononucleares/metabolismo
Leucócitos Mononucleares/parasitologia
Regulação para Cima/imunologia
Células Cultivadas
Animais
Cães
Interferon gama/genética
Macrófagos/metabolismo
Macrófagos/parasitologia
Masculino
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Integrado de Patologia e Biointervenção. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / UFMS-MS. Depto. de Morfofisiologia. Salvador, BA, Brasil
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2014-06-11T17:23:43Z
dc.date.available.fl_str_mv 2014-06-11T17:23:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RODRIGUES, C. A. T. et al. IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro. Veterinary Immunology and Immunopathology, v. 127, n. 3-4, p. 382-388, 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7813
dc.identifier.issn.none.fl_str_mv 0165-2427
dc.identifier.doi.none.fl_str_mv 10.1016/j.vetimm.2008.10.324
identifier_str_mv RODRIGUES, C. A. T. et al. IFN-gamma expression is up-regulated by peripheral blood mononuclear cells (PBMC) from non-exposed dogs upon Leishmania chagasi promastigote stimulation in vitro. Veterinary Immunology and Immunopathology, v. 127, n. 3-4, p. 382-388, 2009.
0165-2427
10.1016/j.vetimm.2008.10.324
url https://www.arca.fiocruz.br/handle/icict/7813
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier B.V
publisher.none.fl_str_mv Elsevier B.V
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/7813/1/Rodrigues%20CAT%20IFN-y%20expression....pdf
https://www.arca.fiocruz.br/bitstream/icict/7813/2/license.txt
https://www.arca.fiocruz.br/bitstream/icict/7813/5/Rodrigues%20CAT%20IFN-y%20expression....pdf.txt
https://www.arca.fiocruz.br/bitstream/icict/7813/4/Rodrigues%20CAT%20IFN-y%20expression....pdf.jpg
bitstream.checksum.fl_str_mv 8deea07db1b090684bd9aeacd243dab0
7d48279ffeed55da8dfe2f8e81f3b81f
ade2d988cbcf4db41935917a694d2769
fe7fd520ae554158c0b5e1f35e866b26
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324823786520576

Registros relacionados