Migration and homing of bone-marrow mononuclear cells in chronic ischemic stroke after intra-arterial injection
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2010 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/37462 |
Resumo: | Sérgio Salles Xavier. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento. |
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Fonseca, Lea Mirian BarbosaGutfilen, BiancaCastro, Paulo Henrique Rosado deBattistella, ValeriaGoldenberg, Regina C. S.Kasai-Brunswick, TaisChagas, Claudia L. R.Wajnberg, EduardoMaiolino, AngeloXavier, Sérgio SallesCharles, AndréMendez-Otero, RosaliaFreitas, Gabriel R.2019-11-28T15:14:39Z2019-11-28T15:14:39Z2010FONSECA, Lea Mirian Barbosa et al. Migration and homing of bone-marrow mononuclear cells in chronic ischemic stroke after intra-arterial injection. Experimental Neurology, v. 221, p. 122-128, 2010.0014-4886https://www.arca.fiocruz.br/handle/icict/3746210.1016/j.expneurol.2009.10.010Sérgio Salles Xavier. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.2020-11-28Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Departamento de Radiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Departamento de Radiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Departamento de Radiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Departamento de Radiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Departamento de Radiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Departamento de Radiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Departamento de Radiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Departamento de Radiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Departamento de Radiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Departamento de Radiologia. Rio de Janeiro, RJ, Brasil.Cell-based treatments have been considered a promising therapy for neurological diseases. However, currently there are no clinically available methods to monitor whether the transplanted cells reach and remain in the brain. In this study we investigated the feasibility of detecting the distribution and homing of autologous bone-marrow mononuclear cells (BMMCs) labeled with Technetium-99 m (99mTc) in a cell-based therapy clinical study for chronic ischemic stroke. Six male patients (ages 24–65 years) with ischemic cerebral infarcts within the middle cerebral artery (MCA) between 59 and 82 days were included. Cell dose ranged from 1.25×108 to 5×108. Approximately 2×107 cells were labeled with 99mTc and intra-arterially delivered together with the unlabeled cells via a catheter navigated to the MCA. None of the patients showed any complications on the 120-day follow-up. Whole body scintigraphies indicated cell homing in the brain of all patients at 2 h, while the remaining uptake was mainly distributed to liver, lungs, spleen, kidneys and bladder. Moreover, quantification of uptake in Single-Photon Emission Computed Tomography (SPECT) at 2 h showed preferential accumulation of radioactivity in the hemisphere affected by the ischemic infarct in all patients. However, at 24 h homing could only distinguished in the brains of 2 patients, while in all patients uptake was still seen in the other organs. Taken together, these results indicate that labeling of BMMCs with 99mTc is a safe and feasible technique that allows monitoring the migration and engraftment of intra-arterially transplanted cells for at least 24 h.engElsevierMigration and homing of bone-marrow mononuclear cells in chronic ischemic stroke after intra-arterial injectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleStrokeCell therapy99mTCScintigraphyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/37462/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Fonseca_Lea_etal_INI_2010.pdfve_Fonseca_Lea_etal_INI_2010.pdfapplication/pdf2444068https://www.arca.fiocruz.br/bitstream/icict/37462/2/ve_Fonseca_Lea_etal_INI_2010.pdf26bdf2f624f4603d9302ea1aea668de3MD52TEXTve_Fonseca_Lea_etal_INI_2010.pdf.txtve_Fonseca_Lea_etal_INI_2010.pdf.txtExtracted texttext/plain46509https://www.arca.fiocruz.br/bitstream/icict/37462/3/ve_Fonseca_Lea_etal_INI_2010.pdf.txt2126b21d757202471f6ed7fca6121b88MD53icict/374622021-03-24 16:32:40.078oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:40Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Migration and homing of bone-marrow mononuclear cells in chronic ischemic stroke after intra-arterial injection |
| title |
Migration and homing of bone-marrow mononuclear cells in chronic ischemic stroke after intra-arterial injection |
| spellingShingle |
Migration and homing of bone-marrow mononuclear cells in chronic ischemic stroke after intra-arterial injection Fonseca, Lea Mirian Barbosa Stroke Cell therapy 99mTC Scintigraphy |
| title_short |
Migration and homing of bone-marrow mononuclear cells in chronic ischemic stroke after intra-arterial injection |
| title_full |
Migration and homing of bone-marrow mononuclear cells in chronic ischemic stroke after intra-arterial injection |
| title_fullStr |
Migration and homing of bone-marrow mononuclear cells in chronic ischemic stroke after intra-arterial injection |
| title_full_unstemmed |
Migration and homing of bone-marrow mononuclear cells in chronic ischemic stroke after intra-arterial injection |
| title_sort |
Migration and homing of bone-marrow mononuclear cells in chronic ischemic stroke after intra-arterial injection |
| author |
Fonseca, Lea Mirian Barbosa |
| author_facet |
Fonseca, Lea Mirian Barbosa Gutfilen, Bianca Castro, Paulo Henrique Rosado de Battistella, Valeria Goldenberg, Regina C. S. Kasai-Brunswick, Tais Chagas, Claudia L. R. Wajnberg, Eduardo Maiolino, Angelo Xavier, Sérgio Salles Charles, André Mendez-Otero, Rosalia Freitas, Gabriel R. |
| author_role |
author |
| author2 |
Gutfilen, Bianca Castro, Paulo Henrique Rosado de Battistella, Valeria Goldenberg, Regina C. S. Kasai-Brunswick, Tais Chagas, Claudia L. R. Wajnberg, Eduardo Maiolino, Angelo Xavier, Sérgio Salles Charles, André Mendez-Otero, Rosalia Freitas, Gabriel R. |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Fonseca, Lea Mirian Barbosa Gutfilen, Bianca Castro, Paulo Henrique Rosado de Battistella, Valeria Goldenberg, Regina C. S. Kasai-Brunswick, Tais Chagas, Claudia L. R. Wajnberg, Eduardo Maiolino, Angelo Xavier, Sérgio Salles Charles, André Mendez-Otero, Rosalia Freitas, Gabriel R. |
| dc.subject.en.pt_BR.fl_str_mv |
Stroke Cell therapy 99mTC Scintigraphy |
| topic |
Stroke Cell therapy 99mTC Scintigraphy |
| description |
Sérgio Salles Xavier. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento. |
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2010 |
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2010 |
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2019-11-28T15:14:39Z |
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2019-11-28T15:14:39Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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FONSECA, Lea Mirian Barbosa et al. Migration and homing of bone-marrow mononuclear cells in chronic ischemic stroke after intra-arterial injection. Experimental Neurology, v. 221, p. 122-128, 2010. |
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https://www.arca.fiocruz.br/handle/icict/37462 |
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0014-4886 |
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10.1016/j.expneurol.2009.10.010 |
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FONSECA, Lea Mirian Barbosa et al. Migration and homing of bone-marrow mononuclear cells in chronic ischemic stroke after intra-arterial injection. Experimental Neurology, v. 221, p. 122-128, 2010. 0014-4886 10.1016/j.expneurol.2009.10.010 |
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