Analysis of ancient DNA from coprolites: a perspective with randon amplified polymorphic DNA-polymerase chain reaction approach

Detalhes bibliográficos
Autor(a) principal: Iñiguez, Alena Mayo
Data de Publicação: 2003
Outros Autores: Araújo, Adauto José Goncalves de, Silva, Luiz Fernando Rocha Ferreira da, Vicente, Ana Carolina Paulo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/34869
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Genética. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ, Brasil.
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spelling Iñiguez, Alena MayoAraújo, Adauto José Goncalves deSilva, Luiz Fernando Rocha Ferreira daVicente, Ana Carolina Paulo2019-08-19T12:58:56Z2019-08-19T12:58:56Z2003IÑIGUEZ, Alena M et al. Analysis of ancient DNA from coprolites: a perspective with random amplified polymorphic DNA-polymerase chain reaction approach. Memórias do Instituto Oswaldo Cruz, v. 98, suplem. 1, p. 1-3, 2003.0074-0276https://www.arca.fiocruz.br/handle/icict/3486910.1590/S0074-027620030009000121678-8060engInstituto Oswaldo CruzAnalysis of ancient DNA from coprolites: a perspective with randon amplified polymorphic DNA-polymerase chain reaction approachinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Genética. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Genética. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ, Brasil.The aim of this work was to determine approaches that would improve the quality of ancient DNA (aDNA) present in coprolites to enhance the possibility of success in retrieving specific sequence targets. We worked with coprolites from South American archaeological sites in Brazil and Chile dating up to 7,000 years ago. Using established protocols for aDNA extraction we obtained samples showing high degradation as usually happens with this kind of material. The reconstructive polymerization pretreatment was essential to overcome the DNA degradation and the serial dilutions helped with to prevent polymerase chain reaction (PCR) inhibitors. Moreover, the random amplified polymorphic DNA-PCR has been shown to be a reliable technique for further experiments to recover specific aDNA sequences.Molecular PaleoparasitologyCoprolitesRandom Amplified Polymorphic DNAAncient DNAPolymerase Chain Reactioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/34869/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALAnalysisDNA.pdfapplication/pdf77466https://www.arca.fiocruz.br/bitstream/icict/34869/2/AnalysisDNA.pdf2f59c2fa166196332dde9efa22e53f8eMD52TEXTAnalysisDNA.pdf.txtAnalysisDNA.pdf.txtExtracted texttext/plain13689https://www.arca.fiocruz.br/bitstream/icict/34869/3/AnalysisDNA.pdf.txtdf8df9dd535e9725d2ae849297b5a535MD53icict/348692019-08-20 02:00:48.041oai:www.arca.fiocruz.br:icict/34869Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-08-20T05:00:48Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Analysis of ancient DNA from coprolites: a perspective with randon amplified polymorphic DNA-polymerase chain reaction approach
title Analysis of ancient DNA from coprolites: a perspective with randon amplified polymorphic DNA-polymerase chain reaction approach
spellingShingle Analysis of ancient DNA from coprolites: a perspective with randon amplified polymorphic DNA-polymerase chain reaction approach
Iñiguez, Alena Mayo
Molecular Paleoparasitology
Coprolites
Random Amplified Polymorphic DNA
Ancient DNA
Polymerase Chain Reaction
title_short Analysis of ancient DNA from coprolites: a perspective with randon amplified polymorphic DNA-polymerase chain reaction approach
title_full Analysis of ancient DNA from coprolites: a perspective with randon amplified polymorphic DNA-polymerase chain reaction approach
title_fullStr Analysis of ancient DNA from coprolites: a perspective with randon amplified polymorphic DNA-polymerase chain reaction approach
title_full_unstemmed Analysis of ancient DNA from coprolites: a perspective with randon amplified polymorphic DNA-polymerase chain reaction approach
title_sort Analysis of ancient DNA from coprolites: a perspective with randon amplified polymorphic DNA-polymerase chain reaction approach
author Iñiguez, Alena Mayo
author_facet Iñiguez, Alena Mayo
Araújo, Adauto José Goncalves de
Silva, Luiz Fernando Rocha Ferreira da
Vicente, Ana Carolina Paulo
author_role author
author2 Araújo, Adauto José Goncalves de
Silva, Luiz Fernando Rocha Ferreira da
Vicente, Ana Carolina Paulo
author2_role author
author
author
dc.contributor.author.fl_str_mv Iñiguez, Alena Mayo
Araújo, Adauto José Goncalves de
Silva, Luiz Fernando Rocha Ferreira da
Vicente, Ana Carolina Paulo
dc.subject.en.pt_BR.fl_str_mv Molecular Paleoparasitology
Coprolites
Random Amplified Polymorphic DNA
Ancient DNA
Polymerase Chain Reaction
topic Molecular Paleoparasitology
Coprolites
Random Amplified Polymorphic DNA
Ancient DNA
Polymerase Chain Reaction
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Genética. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ, Brasil.
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2019-08-19T12:58:56Z
dc.date.available.fl_str_mv 2019-08-19T12:58:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv IÑIGUEZ, Alena M et al. Analysis of ancient DNA from coprolites: a perspective with random amplified polymorphic DNA-polymerase chain reaction approach. Memórias do Instituto Oswaldo Cruz, v. 98, suplem. 1, p. 1-3, 2003.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/34869
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762003000900012
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv IÑIGUEZ, Alena M et al. Analysis of ancient DNA from coprolites: a perspective with random amplified polymorphic DNA-polymerase chain reaction approach. Memórias do Instituto Oswaldo Cruz, v. 98, suplem. 1, p. 1-3, 2003.
0074-0276
10.1590/S0074-02762003000900012
1678-8060
url https://www.arca.fiocruz.br/handle/icict/34869
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz
publisher.none.fl_str_mv Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/34869/1/license.txt
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