Use of ultrasound biomicroscopy to evaluate induced ovarian follicular growth and ovulation in mice.

Detalhes bibliográficos
Autor(a) principal: Campos-Junior, Paulo Henrique de Almeida
Data de Publicação: 2011
Outros Autores: Silva, Cristina Aragão, Grazia, Joao Gabriel Viana, Soares, Milena Botelho Pereira, Santos, Ricardo Ribeiro dos, Viana, Joao Henrique Moreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8983
Resumo: Federal University of Minas Gerais. Belo Horizonte, MG, Brasil / Embrapa Dairy Cattle Research Center. Juiz de Fora, MG, Brasil
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spelling Campos-Junior, Paulo Henrique de AlmeidaSilva, Cristina AragãoGrazia, Joao Gabriel VianaSoares, Milena Botelho PereiraSantos, Ricardo Ribeiro dosViana, Joao Henrique Moreira2014-11-27T17:08:27Z2014-11-27T17:08:27Z2011CAMPOS JUNIOR, P. H. A. et al. Use of ultrasound biomicroscopy to evaluate induced ovarian follicular growth and ovulation in mice. Laboratory Animals, v. 45, n. 4, p. 254-258, 2011.1758-1117https://www.arca.fiocruz.br/handle/icict/898310.1258/la.2011.011031engNature Publishing GroupUse of ultrasound biomicroscopy to evaluate induced ovarian follicular growth and ovulation in mice.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFederal University of Minas Gerais. Belo Horizonte, MG, Brasil / Embrapa Dairy Cattle Research Center. Juiz de Fora, MG, BrasilBiotechnology and Cellular Therapy Center. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFederal University of Juiz de Fora. Juiz de Fora, MG, BrasilBiotechnology and Cellular Therapy Center. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilBiotechnology and Cellular Therapy Center. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilEmbrapa Dairy Cattle Research Center. Juiz de Fora, MG, BrasilRecent advances in image technology, including significant gains in spatial resolution, have made realtime sequential ovarian evaluations possible in small rodents, allowing longitudinal (continued) studies of the ovarian cycle and reducing the required number of experimental animals. The aim of this study was to evaluate exogenous stimulated follicular growth in mice using high-resolution ultrasound technology. Female mice (n ¼ 15) received a 5 IU intraperitoneal injection of equine chorionic gonadotropin (eCG) and 48 h later a 5 IU injection of human chorionic gonadotropin (hCG), and were allowed to mate thereafter. In experiment 1, animals (n ¼ 7) were evaluated every 6 h, from 3 to 51 h after eCG injection, with an ultrasound biomicroscopy (UBM) equipped with a realtime 45 MHz microvisualization probe (RMV 707b). The ovaries were identified and follicular population quantified, and follicles were classified according to the diameter as small ( 449 mm) or large ( 450 mm). A significant change in the distribution of follicle population according to category was observed only 45 h after eCG injection (P, 0.05). In experiment 2, animals (n ¼ 8) were evaluated every 2 h, from 2 h to 10 h after hCG treatment. The largest follicles reached a maximum size (596.7+106.0 mm) 5.8+2.3 h after hCG injection. As expected, the population of large follicles decreased thereafter, indicating the progress of ovulations, but large follicles were still detected late after treatment (10.1+1.1 h). In conclusion, UBM can be used to evaluate follicle dynamics in superstimulated mice (C57BL/6 and BALB/c); significant changes in follicle distribution only occur at later stages after eCG stimulation; and hCG-induced ovulations may not occur synchronously in mice.Follicular dynamicsFolliculogenesisUltrasonographySmall rodentsMicroscopia Acústica/métodosFolículo Ovariano/crescimento & desenvolvimentoFolículo Ovariano/ultrassonografiaOvulação/fisiologiaAnimaisGonadotropina Coriônica/administração & dosagemFemininoCamundongosCamundongos Endogâmicos BALB CFatores de Tempoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8983/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALCampos J r P H A Use of ultrasound....pdfCampos J r P H A Use of ultrasound....pdfapplication/pdf283319https://www.arca.fiocruz.br/bitstream/icict/8983/2/Campos%20J%20r%20P%20H%20A%20Use%20of%20ultrasound....pdf57e446f20b0b9f0ea96574bd03bc1690MD52TEXTCampos J r P H A Use of ultrasound....pdf.txtCampos J r P H A Use of ultrasound....pdf.txtExtracted texttext/plain23574https://www.arca.fiocruz.br/bitstream/icict/8983/3/Campos%20J%20r%20P%20H%20A%20Use%20of%20ultrasound....pdf.txtb28a922656b92c379bad8344cf05894aMD53icict/89832023-03-15 14:33:24.834oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:24Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Use of ultrasound biomicroscopy to evaluate induced ovarian follicular growth and ovulation in mice.
title Use of ultrasound biomicroscopy to evaluate induced ovarian follicular growth and ovulation in mice.
spellingShingle Use of ultrasound biomicroscopy to evaluate induced ovarian follicular growth and ovulation in mice.
Campos-Junior, Paulo Henrique de Almeida
Follicular dynamics
Folliculogenesis
Ultrasonography
Small rodents
Microscopia Acústica/métodos
Folículo Ovariano/crescimento & desenvolvimento
Folículo Ovariano/ultrassonografia
Ovulação/fisiologia
Animais
Gonadotropina Coriônica/administração & dosagem
Feminino
Camundongos
Camundongos Endogâmicos BALB C
Fatores de Tempo
title_short Use of ultrasound biomicroscopy to evaluate induced ovarian follicular growth and ovulation in mice.
title_full Use of ultrasound biomicroscopy to evaluate induced ovarian follicular growth and ovulation in mice.
title_fullStr Use of ultrasound biomicroscopy to evaluate induced ovarian follicular growth and ovulation in mice.
title_full_unstemmed Use of ultrasound biomicroscopy to evaluate induced ovarian follicular growth and ovulation in mice.
title_sort Use of ultrasound biomicroscopy to evaluate induced ovarian follicular growth and ovulation in mice.
author Campos-Junior, Paulo Henrique de Almeida
author_facet Campos-Junior, Paulo Henrique de Almeida
Silva, Cristina Aragão
Grazia, Joao Gabriel Viana
Soares, Milena Botelho Pereira
Santos, Ricardo Ribeiro dos
Viana, Joao Henrique Moreira
author_role author
author2 Silva, Cristina Aragão
Grazia, Joao Gabriel Viana
Soares, Milena Botelho Pereira
Santos, Ricardo Ribeiro dos
Viana, Joao Henrique Moreira
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Campos-Junior, Paulo Henrique de Almeida
Silva, Cristina Aragão
Grazia, Joao Gabriel Viana
Soares, Milena Botelho Pereira
Santos, Ricardo Ribeiro dos
Viana, Joao Henrique Moreira
dc.subject.en.pt_BR.fl_str_mv Follicular dynamics
Folliculogenesis
Ultrasonography
Small rodents
topic Follicular dynamics
Folliculogenesis
Ultrasonography
Small rodents
Microscopia Acústica/métodos
Folículo Ovariano/crescimento & desenvolvimento
Folículo Ovariano/ultrassonografia
Ovulação/fisiologia
Animais
Gonadotropina Coriônica/administração & dosagem
Feminino
Camundongos
Camundongos Endogâmicos BALB C
Fatores de Tempo
dc.subject.decs.pt_BR.fl_str_mv Microscopia Acústica/métodos
Folículo Ovariano/crescimento & desenvolvimento
Folículo Ovariano/ultrassonografia
Ovulação/fisiologia
Animais
Gonadotropina Coriônica/administração & dosagem
Feminino
Camundongos
Camundongos Endogâmicos BALB C
Fatores de Tempo
description Federal University of Minas Gerais. Belo Horizonte, MG, Brasil / Embrapa Dairy Cattle Research Center. Juiz de Fora, MG, Brasil
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-11-27T17:08:27Z
dc.date.available.fl_str_mv 2014-11-27T17:08:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CAMPOS JUNIOR, P. H. A. et al. Use of ultrasound biomicroscopy to evaluate induced ovarian follicular growth and ovulation in mice. Laboratory Animals, v. 45, n. 4, p. 254-258, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8983
dc.identifier.issn.none.fl_str_mv 1758-1117
dc.identifier.doi.none.fl_str_mv 10.1258/la.2011.011031
identifier_str_mv CAMPOS JUNIOR, P. H. A. et al. Use of ultrasound biomicroscopy to evaluate induced ovarian follicular growth and ovulation in mice. Laboratory Animals, v. 45, n. 4, p. 254-258, 2011.
1758-1117
10.1258/la.2011.011031
url https://www.arca.fiocruz.br/handle/icict/8983
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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