17DD and 17D-213/77 yellow fever substrains trigger a balanced cytokine profile in primary vaccinated children
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7382 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil |
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Azevedo, Ana Carolina CampiPorto, Luiza Pacheco de AraújoSilva, Maria LuizaMartins, Marina AngelaAvelar, Renato SathlerCarvalho, Andréa Teixeira deMartins Filho, Olindo AssisBatista, Maurício AzevedoLemos, Denise da SilveiraCamacho, Luiz Antonio BastosMartins, Reinaldo de MenezesMaia, Maria de Lourdes de SousaFarias, Roberto Henrique GuedesFreire, Marcos da SilvaGaller, RicardoHomma, AkiraRibeiro, José Geraldo LeiteLemos, Jandira Aparecida CamposMartins, Maria AuxiliadoraCaldas, Iramaya RodriguesSantos, Silvana Maria Elói2014-03-07T13:46:37Z2014-03-07T13:46:37Z2012CAMPI AZEVEDO, Ana Carolina et al. 17DD and 17D-213/77 yellow fever substrains trigger a balanced cytokine profile in primary vaccinated children. Plos One. 2012, vol.7, n.12, pp. e49828.1932-6203https://www.arca.fiocruz.br/handle/icict/7382engPublic Library of Science17DD and 17D-213/77 yellow fever substrains trigger a balanced cytokine profile in primary vaccinated childreninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrasilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, BrasilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Ouro Preto, MG, BrasilFundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos, Bio-Manguinhos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos, Bio-Manguinhos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos, Bio-Manguinhos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos, Bio-Manguinhos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos, Bio-Manguinhos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos, Bio-Manguinhos. Rio de Janeiro, RJ, BrasilGoverno do Estado de Minas Gerais. Secretaria de Estado de Saúde. Belo Horizonte, MG, BrasilGoverno do Estado de Minas Gerais. Secretaria de Estado de Saúde. Belo Horizonte, MG, BrasilUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Hospital das Clínicas. Ribeirão Preto, SP, BrasilFundação Oswaldo Cruz. Diretoria Regional de Brasília. Brasília, DF, BrasilUniversidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Propedêutica Complementar. Belo Horizonte, MG, BrasilBACKGROUND: This study aimed to compare the cytokine-mediated immune response in children submitted to primary vaccination with the YF-17D-213/77 or YF-17DD yellow fever (YF) substrains. METHODS: A non-probabilistic sample of eighty healthy primary vaccinated (PV) children was selected on the basis of their previously known humoral immune response to the YF vaccines. The selected children were categorized according to their YF-neutralizing antibody titers (PRNT) and referred to as seroconverters (PV-PRNT(+)) or nonseroconverters (PV-PRNT(-)). Following revaccination with the YF-17DD, the PV-PRNT(-) children (YF-17D-213/77 and YF-17DD groups) seroconverted and were referred as RV-PRNT(+). The cytokine-mediated immune response was investigated after short-term in vitro cultures of whole blood samples. The results are expressed as frequency of high cytokine producers, taking the global median of the cytokine index (YF-Ag/control) as the cut-off. RESULTS: The YF-17D-213/77 and the YF-17DD substrains triggered a balanced overall inflammatory/regulatory cytokine pattern in PV-PRNT(+), with a slight predominance of IL-12 in YF-17DD vaccinees and a modest prevalence of IL-10 in YF-17D-213/77. Prominent frequency of neutrophil-derived TNF-α and neutrophils and monocyte-producing IL-12 were the major features of PV-PRNT(+) in the YF-17DD, whereas relevant inflammatory response, mediated by IL-12(+)CD8(+) T cells, was the hallmark of the YF-17D-213/77 vaccinees. Both substrains were able to elicit particular but relevant inflammatory events, regardless of the anti-YF PRNT antibody levels. PV-PRNT(-) children belonging to the YF-17DD arm presented gaps in the inflammatory cytokine signature, especially in terms of the innate immunity, whereas in the YF-17D-213/77 arm the most relevant gap was the deficiency of IL-12-producing CD8(+)T cells. Revaccination with YF-17DD prompted a balanced cytokine profile in YF-17DD nonresponders and a robust inflammatory profile in YF-17D-213/77 nonresponders. CONCLUSION: Our findings demonstrated that, just like the YF-17DD reference vaccine, the YF-17D-213/77 seed lot induced a mixed pattern of inflammatory and regulatory cytokines, supporting its universal use for immunization.cytokine-mediatedchildreninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINAL17DD.pdf17DD.pdfapplication/pdf2852641https://www.arca.fiocruz.br/bitstream/icict/7382/1/17DD.pdfe43b3e257ffaa87a20d7866b9fdc814dMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7382/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXT17DD.pdf.txt17DD.pdf.txtExtracted texttext/plain12https://www.arca.fiocruz.br/bitstream/icict/7382/5/17DD.pdf.txt36c6f0b2061da514c400c0bc2749b5cfMD55THUMBNAIL17DD.pdf.jpg17DD.pdf.jpgGenerated Thumbnailimage/jpeg2199https://www.arca.fiocruz.br/bitstream/icict/7382/4/17DD.pdf.jpgc6f6cbe2342a3bc1a42078dd4c47d691MD54icict/73822023-07-12 11:02:10.16oai:www.arca.fiocruz.br:icict/7382TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-12T14:02:10Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
17DD and 17D-213/77 yellow fever substrains trigger a balanced cytokine profile in primary vaccinated children |
| title |
17DD and 17D-213/77 yellow fever substrains trigger a balanced cytokine profile in primary vaccinated children |
| spellingShingle |
17DD and 17D-213/77 yellow fever substrains trigger a balanced cytokine profile in primary vaccinated children Azevedo, Ana Carolina Campi cytokine-mediated children |
| title_short |
17DD and 17D-213/77 yellow fever substrains trigger a balanced cytokine profile in primary vaccinated children |
| title_full |
17DD and 17D-213/77 yellow fever substrains trigger a balanced cytokine profile in primary vaccinated children |
| title_fullStr |
17DD and 17D-213/77 yellow fever substrains trigger a balanced cytokine profile in primary vaccinated children |
| title_full_unstemmed |
17DD and 17D-213/77 yellow fever substrains trigger a balanced cytokine profile in primary vaccinated children |
| title_sort |
17DD and 17D-213/77 yellow fever substrains trigger a balanced cytokine profile in primary vaccinated children |
| author |
Azevedo, Ana Carolina Campi |
| author_facet |
Azevedo, Ana Carolina Campi Porto, Luiza Pacheco de Araújo Silva, Maria Luiza Martins, Marina Angela Avelar, Renato Sathler Carvalho, Andréa Teixeira de Martins Filho, Olindo Assis Batista, Maurício Azevedo Lemos, Denise da Silveira Camacho, Luiz Antonio Bastos Martins, Reinaldo de Menezes Maia, Maria de Lourdes de Sousa Farias, Roberto Henrique Guedes Freire, Marcos da Silva Galler, Ricardo Homma, Akira Ribeiro, José Geraldo Leite Lemos, Jandira Aparecida Campos Martins, Maria Auxiliadora Caldas, Iramaya Rodrigues Santos, Silvana Maria Elói |
| author_role |
author |
| author2 |
Porto, Luiza Pacheco de Araújo Silva, Maria Luiza Martins, Marina Angela Avelar, Renato Sathler Carvalho, Andréa Teixeira de Martins Filho, Olindo Assis Batista, Maurício Azevedo Lemos, Denise da Silveira Camacho, Luiz Antonio Bastos Martins, Reinaldo de Menezes Maia, Maria de Lourdes de Sousa Farias, Roberto Henrique Guedes Freire, Marcos da Silva Galler, Ricardo Homma, Akira Ribeiro, José Geraldo Leite Lemos, Jandira Aparecida Campos Martins, Maria Auxiliadora Caldas, Iramaya Rodrigues Santos, Silvana Maria Elói |
| author2_role |
author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Azevedo, Ana Carolina Campi Porto, Luiza Pacheco de Araújo Silva, Maria Luiza Martins, Marina Angela Avelar, Renato Sathler Carvalho, Andréa Teixeira de Martins Filho, Olindo Assis Batista, Maurício Azevedo Lemos, Denise da Silveira Camacho, Luiz Antonio Bastos Martins, Reinaldo de Menezes Maia, Maria de Lourdes de Sousa Farias, Roberto Henrique Guedes Freire, Marcos da Silva Galler, Ricardo Homma, Akira Ribeiro, José Geraldo Leite Lemos, Jandira Aparecida Campos Martins, Maria Auxiliadora Caldas, Iramaya Rodrigues Santos, Silvana Maria Elói |
| dc.subject.en.pt_BR.fl_str_mv |
cytokine-mediated children |
| topic |
cytokine-mediated children |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil |
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2012 |
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2012 |
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2014-03-07T13:46:37Z |
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2014-03-07T13:46:37Z |
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CAMPI AZEVEDO, Ana Carolina et al. 17DD and 17D-213/77 yellow fever substrains trigger a balanced cytokine profile in primary vaccinated children. Plos One. 2012, vol.7, n.12, pp. e49828. |
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https://www.arca.fiocruz.br/handle/icict/7382 |
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1932-6203 |
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CAMPI AZEVEDO, Ana Carolina et al. 17DD and 17D-213/77 yellow fever substrains trigger a balanced cytokine profile in primary vaccinated children. Plos One. 2012, vol.7, n.12, pp. e49828. 1932-6203 |
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