PCR assay for identification of histoplasma capsulatum based on the nucleotide sequence of the M antigen

Detalhes bibliográficos
Autor(a) principal: Guedes, H. L.
Data de Publicação: 2003
Outros Autores: Guimarães, A. J., Muniz, M. M., Pizzini, C. V., Hamilton, A. J., Peralta, J. M., Deepe, G. S., Zancopé-Oliveira, R. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33537
Resumo: 2019-12-18
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spelling Guedes, H. L.Guimarães, A. J.Muniz, M. M.Pizzini, C. V.Hamilton, A. J.Peralta, J. M.Deepe, G. S.Zancopé-Oliveira, R. M.2019-06-18T12:49:06Z2019-06-18T12:49:06Z2003GUEDES, Herbert Leonel de Matos et al. PCR assay for identification of histoplasma capsulatum based on the nucleotide sequence of the M antigen. Journal of Clinical Microbiology, v. 41, n. 2, p. 535-539, 2003.0095-1137https://www.arca.fiocruz.br/handle/icict/3353710.1128/JCM.41.2.535–539.20031098-660XengAmerican Society for MicrobiologyPCR assay for identification of histoplasma capsulatum based on the nucleotide sequence of the M antigeninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2019-12-18Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Kings College. Guys Hospital. St. Johns Institute of Dermatology. London, United Kingdom.Universidade Federal do Rio de Janeiro. Rio de Janeiro, RJ, Brasil.University of Cincinnati. College of Medicine. Cincinnati, OH, USA.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.The major diagnostic antigens of Histoplasma capsulatum var. capsulatum are the H and M antigens, pluripotent glycoproteins that elicit both humoral and T-cell-mediated immune responses. The gene encoding the M antigen has previously been sequenced, and its sequence has significant overall homology to those of the genes for fungal catalases. Regions of the M-antigen gene with little or no homology were used to design four oligonucleotide sequences for application in the PCR detection and identification of H. capsulatum var. capsulatum. The PCR correctly identified the 31 H. capsulatum var. capsulatum strains isolated from human, animal, and soil specimens and 1 H. capsulatum var. duboisii isolate. PCR products of 111 and 279 bp were amplified with primers Msp1F-Msp1R and Msp2F-Msp2R, respectively. No amplification product was obtained from DNA extracted from an H. capsulatum var. farciminosum isolate. The specificity of the PCR with the M-antigen-derived primers was confirmed by the total absence of amplification products when genomic DNA from Paracoccidioides brasiliensis, Candida spp., Sporothrix schenckii, Cryptococcus neoformans, Blastomyces dermatitidis, Coccidioides immitis, Aspergillus niger, and Aspergillus fumigatus were applied in the reaction. This rapid, sensitive, and specific assay provides a way to identify typical and atypical isolates of H. capsulatum var. capsulatum.Histoplasma capsulatumM antigenPCRinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/33537/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Reiss_E_etal_INI_2000.pdfve_Reiss_E_etal_INI_2000.pdfapplication/pdf279268https://www.arca.fiocruz.br/bitstream/icict/33537/2/ve_Reiss_E_etal_INI_2000.pdfa08261f72919b04fbf317089e094553cMD52TEXTve_Reiss_E_etal_INI_2000.pdf.txtve_Reiss_E_etal_INI_2000.pdf.txtExtracted texttext/plain71356https://www.arca.fiocruz.br/bitstream/icict/33537/3/ve_Reiss_E_etal_INI_2000.pdf.txtd6a6bca57c764b729bc730d07638c9b8MD53icict/335372021-03-24 16:32:25.732oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:25Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv PCR assay for identification of histoplasma capsulatum based on the nucleotide sequence of the M antigen
title PCR assay for identification of histoplasma capsulatum based on the nucleotide sequence of the M antigen
spellingShingle PCR assay for identification of histoplasma capsulatum based on the nucleotide sequence of the M antigen
Guedes, H. L.
Histoplasma capsulatum
M antigen
PCR
title_short PCR assay for identification of histoplasma capsulatum based on the nucleotide sequence of the M antigen
title_full PCR assay for identification of histoplasma capsulatum based on the nucleotide sequence of the M antigen
title_fullStr PCR assay for identification of histoplasma capsulatum based on the nucleotide sequence of the M antigen
title_full_unstemmed PCR assay for identification of histoplasma capsulatum based on the nucleotide sequence of the M antigen
title_sort PCR assay for identification of histoplasma capsulatum based on the nucleotide sequence of the M antigen
author Guedes, H. L.
author_facet Guedes, H. L.
Guimarães, A. J.
Muniz, M. M.
Pizzini, C. V.
Hamilton, A. J.
Peralta, J. M.
Deepe, G. S.
Zancopé-Oliveira, R. M.
author_role author
author2 Guimarães, A. J.
Muniz, M. M.
Pizzini, C. V.
Hamilton, A. J.
Peralta, J. M.
Deepe, G. S.
Zancopé-Oliveira, R. M.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Guedes, H. L.
Guimarães, A. J.
Muniz, M. M.
Pizzini, C. V.
Hamilton, A. J.
Peralta, J. M.
Deepe, G. S.
Zancopé-Oliveira, R. M.
dc.subject.en.pt_BR.fl_str_mv Histoplasma capsulatum
M antigen
PCR
topic Histoplasma capsulatum
M antigen
PCR
description 2019-12-18
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2019-06-18T12:49:06Z
dc.date.available.fl_str_mv 2019-06-18T12:49:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GUEDES, Herbert Leonel de Matos et al. PCR assay for identification of histoplasma capsulatum based on the nucleotide sequence of the M antigen. Journal of Clinical Microbiology, v. 41, n. 2, p. 535-539, 2003.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33537
dc.identifier.issn.pt_BR.fl_str_mv 0095-1137
dc.identifier.doi.none.fl_str_mv 10.1128/JCM.41.2.535–539.2003
dc.identifier.eissn.none.fl_str_mv 1098-660X
identifier_str_mv GUEDES, Herbert Leonel de Matos et al. PCR assay for identification of histoplasma capsulatum based on the nucleotide sequence of the M antigen. Journal of Clinical Microbiology, v. 41, n. 2, p. 535-539, 2003.
0095-1137
10.1128/JCM.41.2.535–539.2003
1098-660X
url https://www.arca.fiocruz.br/handle/icict/33537
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/33537/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/33537/2/ve_Reiss_E_etal_INI_2000.pdf
https://www.arca.fiocruz.br/bitstream/icict/33537/3/ve_Reiss_E_etal_INI_2000.pdf.txt
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