Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum

Detalhes bibliográficos
Autor(a) principal: Guimarães, Allan Jefferson
Data de Publicação: 2008
Outros Autores: Hamilton, Andrew John, Guedes, Herbert Leonel de M., Nosanchuk, Joshua Daniel, Zancopé-Oliveira, Rosely Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32603
Resumo: Albert Einstein College of Medicine of Yeshiva University. Department of Medicine and Microbiology and Immunology. Division of Infectious Diseases. Bronx, NY, USA / Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil.
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spelling Guimarães, Allan JeffersonHamilton, Andrew JohnGuedes, Herbert Leonel de M.Nosanchuk, Joshua DanielZancopé-Oliveira, Rosely Maria2019-04-18T15:32:19Z2019-04-18T15:32:19Z2008GUIMARÃES, Allan Jefferson et al. Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum. Plos One, v. 3, n. 10, p. 1-17, Oct. 2008.1932-6203https://www.arca.fiocruz.br/handle/icict/3260310.1371/journal.pone.0003449engPublic Library of ScienceHistoplasma capsulatumAntígeno MFunção BiológicaMapeamento MolecularFase de LeveduraHistoplasma capsulatumM antigenBiological FunctionMolecular MappingYeast PhaseBiological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAlbert Einstein College of Medicine of Yeshiva University. Department of Medicine and Microbiology and Immunology. Division of Infectious Diseases. Bronx, NY, USA / Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil.St John’s Institute of Dermatology. Guy’s Hospital. King’s College, London, United Kingdom.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ, Brasil.Albert Einstein College of Medicine of Yeshiva University. Department of Medicine and Microbiology and Immunology. Division of Infectious Diseases. Bronx, NY, USA.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil.Histoplasmosis, due to the intracellular fungus Histoplasma capsulatum, can be diagnosed by demonstrating the presence of antibodies specific to the immunodominant M antigen. However, the role of this protein in the pathogenesis of histoplasmosis has not been elucidated. We sought to structurally and immunologically characterize the protein, determine yeast cell surface expression, and confirm catalase activity. A 3D-rendering of the M antigen by homology modeling revealed that the structures and domains closely resemble characterized fungal catalases. We generated monoclonal antibodies (mAbs) to the protein and determined that the M antigen is present on the yeast cell surface and in cell wall/cell membrane preparations. Similarly, we found that the majority of catalase activity was in extracts containing fungal surface antigens and that the M antigen is not significantly secreted by live yeast cells. The mAbs also identified unique epitopes on the M antigen. The localization of the M antigen to the cell surface of H. capsulatum yeast and the characterization of the protein’s major epitopes have important implications since it demonstrates that although the protein may participate in protecting the fungus against oxidative stress it is also accessible to host immune cells and antibody.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/32603/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALHerbertLeonel_Guedes_etal_IOC_2008.pdfHerbertLeonel_Guedes_etal_IOC_2008.pdfapplication/pdf982729https://www.arca.fiocruz.br/bitstream/icict/32603/2/HerbertLeonel_Guedes_etal_IOC_2008.pdfcfffebcdf26c41c057d3a09ee177923dMD52TEXTHerbertLeonel_Guedes_etal_IOC_2008.pdf.txtHerbertLeonel_Guedes_etal_IOC_2008.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum
title Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum
spellingShingle Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum
Guimarães, Allan Jefferson
Histoplasma capsulatum
Antígeno M
Função Biológica
Mapeamento Molecular
Fase de Levedura
Histoplasma capsulatum
M antigen
Biological Function
Molecular Mapping
Yeast Phase
title_short Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum
title_full Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum
title_fullStr Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum
title_full_unstemmed Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum
title_sort Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum
author Guimarães, Allan Jefferson
author_facet Guimarães, Allan Jefferson
Hamilton, Andrew John
Guedes, Herbert Leonel de M.
Nosanchuk, Joshua Daniel
Zancopé-Oliveira, Rosely Maria
author_role author
author2 Hamilton, Andrew John
Guedes, Herbert Leonel de M.
Nosanchuk, Joshua Daniel
Zancopé-Oliveira, Rosely Maria
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Guimarães, Allan Jefferson
Hamilton, Andrew John
Guedes, Herbert Leonel de M.
Nosanchuk, Joshua Daniel
Zancopé-Oliveira, Rosely Maria
dc.subject.other.pt_BR.fl_str_mv Histoplasma capsulatum
Antígeno M
Função Biológica
Mapeamento Molecular
Fase de Levedura
topic Histoplasma capsulatum
Antígeno M
Função Biológica
Mapeamento Molecular
Fase de Levedura
Histoplasma capsulatum
M antigen
Biological Function
Molecular Mapping
Yeast Phase
dc.subject.en.pt_BR.fl_str_mv Histoplasma capsulatum
M antigen
Biological Function
Molecular Mapping
Yeast Phase
description Albert Einstein College of Medicine of Yeshiva University. Department of Medicine and Microbiology and Immunology. Division of Infectious Diseases. Bronx, NY, USA / Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil.
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2019-04-18T15:32:19Z
dc.date.available.fl_str_mv 2019-04-18T15:32:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GUIMARÃES, Allan Jefferson et al. Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum. Plos One, v. 3, n. 10, p. 1-17, Oct. 2008.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32603
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0003449
identifier_str_mv GUIMARÃES, Allan Jefferson et al. Biological Function and Molecular Mapping of M Antigen in Yeast Phase of Histoplasma capsulatum. Plos One, v. 3, n. 10, p. 1-17, Oct. 2008.
1932-6203
10.1371/journal.pone.0003449
url https://www.arca.fiocruz.br/handle/icict/32603
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/32603/3/HerbertLeonel_Guedes_etal_IOC_2008.pdf.txt
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