Performance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infection
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/26532 |
Resumo: | Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biologicas. Nucleo de Pesquisas em Ciências Biologicas. Laboratorio de Doença de Chagas. Ouro Preto, MG, Brazil |
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Alessio, Glaucia DinizAraujo, Fernanda Fortes deCortes, Denise FonsecaSales Junior, Policarpo AdemarLima, Daniela CristinaGomes, Matheus de SouzaAmaral, Laurence Rodrigues doXavier, Marcelo Antonio PascoalCarvalho, Andrea Teixeira deMartins Filho, Olindo AssisLana, Marta de2018-05-21T18:05:11Z2018-05-21T18:05:11Z2017ALESSIO, Glaucia Diniz et al. Performance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infection. PLoS Neglected Tropical Diseases, v. 11, p. e0005444-24, 20171935-2727https://www.arca.fiocruz.br/handle/icict/2653210.1371/journal.pntd.0005444engPublic Library of ScienceTrypanosoma cruziDoença de ChagasTrypanosoma cruziChagas diseasePerformance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biologicas. Nucleo de Pesquisas em Ciências Biologicas. Laboratorio de Doença de Chagas. Ouro Preto, MG, BrazilFundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, BrazilUniversidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biologicas. Nucleo de Pesquisas em Ciências Biologicas. Laboratorio de Doença de Chagas. Ouro Preto, MG, BrazilFundação Oswaldo Cruz. Instituto Rene Rachou. Grupo de Genomica Funcional e Proteomica de Leishmania spp e Trypanosoma cruzi. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, BrazilUniversidade Federal de Uberlandia. Laboratorio de Bioinformatica e Analises Moleculares. Patos de Minas, MG, BrazilUniversidade Federal de Uberlandia. Laboratorio de Bioinformatica e Analises Moleculares. Patos de Minas, MG, BrazilFundação Oswaldo Cruz. Instituto Rene Rachou. Grupo de Pesquisas Clınicas e Polıticas Publicas em Doenças Infecciosas e Parasitarias. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, BrazilUniversidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biologicas. Nucleo de Pesquisas em Ciências Biologicas. Laboratorio de Doença de Chagas. Ouro Preto, MG, BrazilDistinct Trypanosoma cruzi genotypes have been considered relevant for patient management and therapeutic response of Chagas disease. However, typing strategies for genotype- specific serodiagnosis of Chagas disease are still unavailable and requires standardization for practical application. In this study, an innovative TcI/TcVI/TcII Chagas Flow ATE-IgG2a technique was developed with applicability for universal and genotypespecific diagnosis of T. cruzi infection. For this purpose, the reactivity of serum samples (percentage of positive fluorescent parasites-PPFP) obtained from mice chronically infected with TcI/Colombiana, TcVI/CL or TcII/Y strain as well as non-infected controls were determined using amastigote-AMA, trypomastigote-TRYPO and epimastigote-EPI in parallel batches of TcI, TcVI and TcII target antigens. Data demonstrated that ªα-TcII-TRYPO/ 1:500, cut-off/PPFP = 20%º presented an excellent performance for universal diagnosis of T. cruzi infection (AUC = 1.0, Se and Sp = 100%). The combined set of attributes ªα-TcITRYPO/ 1:4,000, cut-off/PPFP = 50%º, ªα-TcII-AMA/1:1,000, cut-off/PPFP = 40%º and ªα- TcVI-EPI/1:1,000, cut-off/PPFP = 45%º showed good performance to segregate infections with TcI/Colombiana, TcVI/CL or TcII/Y strain. Overall, hosts infected with TcI/Colombiana and TcII/Y strains displayed opposite patterns of reactivity with ªα-TcI TRYPOº and ªα-TcII AMAº. Hosts infected with TcVI/CL strain showed a typical interweaved distribution pattern. The method presented a good performance for genotype-specific diagnosis, with global accuracy of 69% when the population/prototype scenario include TcI, TcVI and TcII infections and 94% when comprise only TcI and TcII infections. This study also proposes a receiver operating reactivity panel, providing a feasible tool to classify serum samples from hosts infected with distinct T. cruzi genotypes, supporting the potential of this method for universal and genotype-specific diagnosis of T. cruzi infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/26532/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALPerformance of TcI.pdfPerformance of TcI.pdfapplication/pdf2617470https://www.arca.fiocruz.br/bitstream/icict/26532/2/Performance%20of%20TcI.pdf52179026c76fa1220f673af14916aa9aMD52TEXTPerformance of TcI.pdf.txtPerformance of TcI.pdf.txtExtracted texttext/plain85245https://www.arca.fiocruz.br/bitstream/icict/26532/3/Performance%20of%20TcI.pdf.txta453b7dd0c6bf75ad9cb911a06311125MD53icict/265322019-06-19 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| dc.title.pt_BR.fl_str_mv |
Performance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infection |
| title |
Performance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infection |
| spellingShingle |
Performance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infection Alessio, Glaucia Diniz Trypanosoma cruzi Doença de Chagas Trypanosoma cruzi Chagas disease |
| title_short |
Performance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infection |
| title_full |
Performance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infection |
| title_fullStr |
Performance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infection |
| title_full_unstemmed |
Performance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infection |
| title_sort |
Performance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infection |
| author |
Alessio, Glaucia Diniz |
| author_facet |
Alessio, Glaucia Diniz Araujo, Fernanda Fortes de Cortes, Denise Fonseca Sales Junior, Policarpo Ademar Lima, Daniela Cristina Gomes, Matheus de Souza Amaral, Laurence Rodrigues do Xavier, Marcelo Antonio Pascoal Carvalho, Andrea Teixeira de Martins Filho, Olindo Assis Lana, Marta de |
| author_role |
author |
| author2 |
Araujo, Fernanda Fortes de Cortes, Denise Fonseca Sales Junior, Policarpo Ademar Lima, Daniela Cristina Gomes, Matheus de Souza Amaral, Laurence Rodrigues do Xavier, Marcelo Antonio Pascoal Carvalho, Andrea Teixeira de Martins Filho, Olindo Assis Lana, Marta de |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Alessio, Glaucia Diniz Araujo, Fernanda Fortes de Cortes, Denise Fonseca Sales Junior, Policarpo Ademar Lima, Daniela Cristina Gomes, Matheus de Souza Amaral, Laurence Rodrigues do Xavier, Marcelo Antonio Pascoal Carvalho, Andrea Teixeira de Martins Filho, Olindo Assis Lana, Marta de |
| dc.subject.other.pt_BR.fl_str_mv |
Trypanosoma cruzi Doença de Chagas |
| topic |
Trypanosoma cruzi Doença de Chagas Trypanosoma cruzi Chagas disease |
| dc.subject.en.pt_BR.fl_str_mv |
Trypanosoma cruzi Chagas disease |
| description |
Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biologicas. Nucleo de Pesquisas em Ciências Biologicas. Laboratorio de Doença de Chagas. Ouro Preto, MG, Brazil |
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2017 |
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2017 |
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2018-05-21T18:05:11Z |
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2018-05-21T18:05:11Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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ALESSIO, Glaucia Diniz et al. Performance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infection. PLoS Neglected Tropical Diseases, v. 11, p. e0005444-24, 2017 |
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https://www.arca.fiocruz.br/handle/icict/26532 |
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1935-2727 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pntd.0005444 |
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ALESSIO, Glaucia Diniz et al. Performance of TcI/TcVI/TcII Chagas-Flow ATE-IgG2a for universal and genotype-specific serodiagnosis of Trypanosoma cruzi infection. PLoS Neglected Tropical Diseases, v. 11, p. e0005444-24, 2017 1935-2727 10.1371/journal.pntd.0005444 |
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eng |
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Public Library of Science |
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Public Library of Science |
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