Accomplishing the genotype-specific serodiagnosis of single and dual Trypanosoma cruzi infections by flow cytometry Chagas- Flow ATE-IgG2a

Detalhes bibliográficos
Autor(a) principal: Alessio, Glaucia Diniz
Data de Publicação: 2018
Outros Autores: Araujo, Fernanda Fortes de, Sales Junior, Policarpo Ademar, Gomes, Matheus de Souza, Amaral, Laurence Rodrigues do, Xavier, Marcelo Antonio Pascoal, Carvalho, Andrea Teixeira de, Lana, Marta de, Martins Filho, Olindo Assis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/28813
Resumo: Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biologicas. Nucleo de Pesquisas em Ciências Biologicas. Laboratorio de Doença de Chagas. Ouro Preto, MG, Brazil/Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
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spelling Alessio, Glaucia DinizAraujo, Fernanda Fortes deSales Junior, Policarpo AdemarGomes, Matheus de SouzaAmaral, Laurence Rodrigues doXavier, Marcelo Antonio PascoalCarvalho, Andrea Teixeira deLana, Marta deMartins Filho, Olindo Assis2018-09-17T12:19:39Z2018-09-17T12:19:39Z2018ALESSIO, Glaucia Diniz et al. Accomplishing the genotype-specific serodiagnosis of single and dual Trypanosoma cruzi infections by flow cytometry Chagas- Flow ATE-IgG2a. PLoS Neglected Tropical Diseases, v. 12, p. 1-23, 2018.1935-2735https://www.arca.fiocruz.br/handle/icict/2881310.1371/journal.pntd.0006140engPublic Library of ScienceTrypanosoma cruzidoenças parasitáriasÁrvores de decisãodoença de chagassorodiagnosticosangueCitometria de fluxotripomastigotasTrypanosoma cruziParasitic diseasesDecision treesChagas diseaseSerodiagnosisBloodFlow cytometryTrypomastigotesAccomplishing the genotype-specific serodiagnosis of single and dual Trypanosoma cruzi infections by flow cytometry Chagas- Flow ATE-IgG2ainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biologicas. Nucleo de Pesquisas em Ciências Biologicas. Laboratorio de Doença de Chagas. Ouro Preto, MG, Brazil/Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil/Universidade de Uberaba. Programa de Pos-graduacão em Sanidade e Producão Animal nos Tropicos. Uberaba, MG, BrazilFundação Oswaldo Cruz. Instituto Rene Rachou. Grupo de Genomica Funcional e Proteomica de Leishmania spp e Trypanosoma cruzi. Belo Horizonte, MG, BrazilUniversidade Federal de Uberlandia. Laboratorio de Bioinformatica e Analises Moleculares. Patos de Minas, MG, BrazilUniversidade Federal de Uberlandia. Laboratorio de Bioinformatica e Analises Moleculares. Patos de Minas, MG, BrazilFundação Oswaldo Cruz. Instituto Rene Rachou. Grupo de Pesquisas Clınicas e Polıticas Publicas em Doencas Infecciosas e Parasitarias. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, BrazilUniversidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biologicas. Nucleo de Pesquisas em Ciências Biologicas. Laboratorio de Doença de Chagas. Ouro Preto, MG, BrazilFundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, BrazilThe methods currently available for genotype-specific diagnosis of T. cruzi infection still present relevant limitations, especially to identify mixed infection. In the present investigation, we have evaluated the performance of Chagas-Flow ATE-IgG2a test for early and late differential diagnosis of single and dual genotype-specific T. cruzi infections. Serum samples from Swiss mice at early and late stages of T. cruzi infection were assayed in parallel batches for genotype-specific diagnosis of single (TcI, TcVI or TcII) and dual (TcI+TcVI, TcVI+TcII or TcII+TcI) infections. The intrinsic reactivity to TcI, TcVI and TcII target antigens, including amastigote (AI/AVI/AII), trypomastigote-(TI/TVI/TII) and epimastigote (EI/ EVI/EII), at specific reverse of serum dilutions (500 to 64,000), was employed to provide reliable decision-trees for ªearlyº vs ªlateº, ªsingle vs ªdualº and ªgenotype-specificº serology. The results demonstrated that selective set of attributes ªEII 500/EI 2,000/AII 500º were able to provide high-quality accuracy (81%) to segregate early and late stages of T. cruzi infection. The sets ªTI 2,000/AI 1,000/EII 1,000º and ªTI 8,000/AII 32,000º presented expressive scores to discriminate single from dual T. cruzi infections at early (85%) and late stages (84%), respectively. Moreover, the attributes ªTI 4,000/TVI 500/TII 1,000º, ªTI 16,000/EI 2,000/EII 2,000/AI 500/TVI 500º showed good performance for genotype-specific diagnosis at early stage of single (72%) and dual (80%) T. cruzi infections, respectively. In addition, the attributes ªTI 4,000/AII 1,000/EVI 1,000º, ªTI 64,000/AVI 500/AI 2,000/AII 1,000/EII 4,000º showed moderate performance for genotype-specific diagnosis at late stage of single (69%) and dual (76%) T. cruzi infections, respectively. The sets of decision-trees were assembled to construct a sequential algorithm with expressive accuracy (81%) for serological diagnosis of T. cruzi infection. These findings engender new perspectives for the application of Chagas-Flow ATE-IgG2a method for genotype-specific diagnosis in humans, with relevant contributions for epidemiological surveys as well as clinical and post-therapeutic monitoring of Chagas disease.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/28813/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALAccomplishing the genotype-specific serodiagnosis.pdfAccomplishing the genotype-specific serodiagnosis.pdfapplication/pdf7853531https://www.arca.fiocruz.br/bitstream/icict/28813/2/Accomplishing%20the%20genotype-specific%20serodiagnosis.pdf60240bef7d72804032b24744413a6f53MD52TEXTAccomplishing the genotype-specific serodiagnosis.pdf.txtAccomplishing the genotype-specific serodiagnosis.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Accomplishing the genotype-specific serodiagnosis of single and dual Trypanosoma cruzi infections by flow cytometry Chagas- Flow ATE-IgG2a
title Accomplishing the genotype-specific serodiagnosis of single and dual Trypanosoma cruzi infections by flow cytometry Chagas- Flow ATE-IgG2a
spellingShingle Accomplishing the genotype-specific serodiagnosis of single and dual Trypanosoma cruzi infections by flow cytometry Chagas- Flow ATE-IgG2a
Alessio, Glaucia Diniz
Trypanosoma cruzi
doenças parasitárias
Árvores de decisão
doença de chagas
sorodiagnostico
sangue
Citometria de fluxo
tripomastigotas
Trypanosoma cruzi
Parasitic diseases
Decision trees
Chagas disease
Serodiagnosis
Blood
Flow cytometry
Trypomastigotes
title_short Accomplishing the genotype-specific serodiagnosis of single and dual Trypanosoma cruzi infections by flow cytometry Chagas- Flow ATE-IgG2a
title_full Accomplishing the genotype-specific serodiagnosis of single and dual Trypanosoma cruzi infections by flow cytometry Chagas- Flow ATE-IgG2a
title_fullStr Accomplishing the genotype-specific serodiagnosis of single and dual Trypanosoma cruzi infections by flow cytometry Chagas- Flow ATE-IgG2a
title_full_unstemmed Accomplishing the genotype-specific serodiagnosis of single and dual Trypanosoma cruzi infections by flow cytometry Chagas- Flow ATE-IgG2a
title_sort Accomplishing the genotype-specific serodiagnosis of single and dual Trypanosoma cruzi infections by flow cytometry Chagas- Flow ATE-IgG2a
author Alessio, Glaucia Diniz
author_facet Alessio, Glaucia Diniz
Araujo, Fernanda Fortes de
Sales Junior, Policarpo Ademar
Gomes, Matheus de Souza
Amaral, Laurence Rodrigues do
Xavier, Marcelo Antonio Pascoal
Carvalho, Andrea Teixeira de
Lana, Marta de
Martins Filho, Olindo Assis
author_role author
author2 Araujo, Fernanda Fortes de
Sales Junior, Policarpo Ademar
Gomes, Matheus de Souza
Amaral, Laurence Rodrigues do
Xavier, Marcelo Antonio Pascoal
Carvalho, Andrea Teixeira de
Lana, Marta de
Martins Filho, Olindo Assis
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Alessio, Glaucia Diniz
Araujo, Fernanda Fortes de
Sales Junior, Policarpo Ademar
Gomes, Matheus de Souza
Amaral, Laurence Rodrigues do
Xavier, Marcelo Antonio Pascoal
Carvalho, Andrea Teixeira de
Lana, Marta de
Martins Filho, Olindo Assis
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
doenças parasitárias
Árvores de decisão
doença de chagas
sorodiagnostico
sangue
Citometria de fluxo
tripomastigotas
topic Trypanosoma cruzi
doenças parasitárias
Árvores de decisão
doença de chagas
sorodiagnostico
sangue
Citometria de fluxo
tripomastigotas
Trypanosoma cruzi
Parasitic diseases
Decision trees
Chagas disease
Serodiagnosis
Blood
Flow cytometry
Trypomastigotes
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Parasitic diseases
Decision trees
Chagas disease
Serodiagnosis
Blood
Flow cytometry
Trypomastigotes
description Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biologicas. Nucleo de Pesquisas em Ciências Biologicas. Laboratorio de Doença de Chagas. Ouro Preto, MG, Brazil/Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-09-17T12:19:39Z
dc.date.available.fl_str_mv 2018-09-17T12:19:39Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALESSIO, Glaucia Diniz et al. Accomplishing the genotype-specific serodiagnosis of single and dual Trypanosoma cruzi infections by flow cytometry Chagas- Flow ATE-IgG2a. PLoS Neglected Tropical Diseases, v. 12, p. 1-23, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/28813
dc.identifier.issn.pt_BR.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0006140
identifier_str_mv ALESSIO, Glaucia Diniz et al. Accomplishing the genotype-specific serodiagnosis of single and dual Trypanosoma cruzi infections by flow cytometry Chagas- Flow ATE-IgG2a. PLoS Neglected Tropical Diseases, v. 12, p. 1-23, 2018.
1935-2735
10.1371/journal.pntd.0006140
url https://www.arca.fiocruz.br/handle/icict/28813
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
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