Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease

Detalhes bibliográficos
Autor(a) principal: Alessio, Glaucia Diniz
Data de Publicação: 2020
Outros Autores: Araújo, Fernanda Fortes de, Silva, Jéssica Spínola, Sales Júnior, Policarpo Ademar, Gomes, Matheus de Souza, Amaral, Laurence Rodrigues do, Ramírez, Juan David, Flórez, Carolina, Carvalho, Andréa Teixeira de, Pinheiro, Melina de Barros, Lana, Marta de, Martins Filho, Olindo Assis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44081
Resumo: Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil/ Universidade Federal de São João Del-Rei. Histopatologia e Análises Clínicas. Laboratório CT-Infra II. Divinópolis, MG, Brazil
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spelling Alessio, Glaucia DinizAraújo, Fernanda Fortes deSilva, Jéssica SpínolaSales Júnior, Policarpo AdemarGomes, Matheus de SouzaAmaral, Laurence Rodrigues doRamírez, Juan DavidFlórez, CarolinaCarvalho, Andréa Teixeira dePinheiro, Melina de BarrosLana, Marta deMartins Filho, Olindo Assis2020-10-20T15:58:37Z2020-10-20T15:58:37Z2020ALESSIO, Glaucia Diniz et al. Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease. Sci Rep, v. 10, 13296, 2020. https://doi.org/10.1038/s41598-020-69921-z2045-2322https://www.arca.fiocruz.br/handle/icict/4408110.1038/s41598-020-69921-zengNatureDoença de chagasTrypanosoma cruziChagas diseaseTrypanosoma cruziHuman Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas diseaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil/ Universidade Federal de São João Del-Rei. Histopatologia e Análises Clínicas. Laboratório CT-Infra II. Divinópolis, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, BrazilUniversidade Federal de São João Del-Rei. Histopatologia e Análises Clínicas. Laboratório CT-Infra II. Divinópolis, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Grupo de Genômica Funcional e Proteômica de Leishmania spp e Trypanosoma cruzi. Belo Horizonte, MG, BrazilUniversidade Federal de Uberlândia. Rede Multidisciplinar de Pesquisa, Ciência e Tecnologia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, BrazilUniversidade Federal de Uberlândia. Rede Multidisciplinar de Pesquisa, Ciência e Tecnologia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, BrazilUniversidad del Rosario. Facultad de Ciencias Naturales. Departamento de Biología. Grupo de Investigaciones Microbiológicas-UR. Bogotá, ColombiaInstituto Nacional de Salud. Bogotá, ColombiaFundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, BrazilUniversidade Federal de São João Del-Rei. Histopatologia e Análises Clínicas. Laboratório CT-Infra II. Divinópolis, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil/ Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Doença de Chagas Ouro Preto, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, BrazilThe molecular and serological methods available for Discrete Typing Units (DTU)-specific diagnosis of Trypanosoma cruzi in chronic Chagas disease present limitations. The study evaluated the performance of Human Chagas-Flow ATE-IgG1 for universal and DTU-specific diagnosis of Chagas disease. A total of 102 sera from Chagas disease patients (CH) chronically infected with TcI, TcVI or TcII DTUs were tested for IgG1 reactivity to amastigote/(A), trypomastigote/(T) and epimastigote/(E) antigens along the titration curve (1:250–1:32,000). The results demonstrated that “AI 250/40%”, “EVI 250/30%”, “AII 250/40%”, “TII 250/40%” and “EII 250/30%” have outstanding accuracy (100%) to segregate CH from non-infected controls. The attributes “TI 4,000/50%”, “EI 2,000/50%”, “AVI 8,000/60%” and “TVI 4,000/50%” were selected for DTU-specific serotyping of Chagas disease. The isolated use of “EI 2,000/50%” provided the highest co-positivity for TcI patients (91%). The combined decision tree algorithms using the pre-defined sets of attributes showed outstanding full accuracy (92% and 97%) to discriminate “TcI vs TcVI vs TcII” and “TcI vs TcII” prototypes, respectively. The elevated performance of Human Chagas-Flow ATE-IgG1 qualifies its use for universal and TcI/TcVI/TcII-specific diagnosis of Chagas disease. These findings further support the application of this method in epidemiological surveys, post-therapeutic monitoring and clinical outcome follow-ups for Chagas disease.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/44081/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALHuman Chagas-Flow ATE-IgG1 for advanced.pdfHuman Chagas-Flow ATE-IgG1 for advanced.pdfapplication/pdf1982491https://www.arca.fiocruz.br/bitstream/icict/44081/2/Human%20Chagas-Flow%20ATE-IgG1%20for%20advanced.pdfefb014901fbcd8e7d6cac72b47210387MD52TEXTHuman Chagas-Flow ATE-IgG1 for advanced.pdf.txtHuman Chagas-Flow ATE-IgG1 for advanced.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease
title Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease
spellingShingle Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease
Alessio, Glaucia Diniz
Doença de chagas
Trypanosoma cruzi
Chagas disease
Trypanosoma cruzi
title_short Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease
title_full Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease
title_fullStr Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease
title_full_unstemmed Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease
title_sort Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease
author Alessio, Glaucia Diniz
author_facet Alessio, Glaucia Diniz
Araújo, Fernanda Fortes de
Silva, Jéssica Spínola
Sales Júnior, Policarpo Ademar
Gomes, Matheus de Souza
Amaral, Laurence Rodrigues do
Ramírez, Juan David
Flórez, Carolina
Carvalho, Andréa Teixeira de
Pinheiro, Melina de Barros
Lana, Marta de
Martins Filho, Olindo Assis
author_role author
author2 Araújo, Fernanda Fortes de
Silva, Jéssica Spínola
Sales Júnior, Policarpo Ademar
Gomes, Matheus de Souza
Amaral, Laurence Rodrigues do
Ramírez, Juan David
Flórez, Carolina
Carvalho, Andréa Teixeira de
Pinheiro, Melina de Barros
Lana, Marta de
Martins Filho, Olindo Assis
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Alessio, Glaucia Diniz
Araújo, Fernanda Fortes de
Silva, Jéssica Spínola
Sales Júnior, Policarpo Ademar
Gomes, Matheus de Souza
Amaral, Laurence Rodrigues do
Ramírez, Juan David
Flórez, Carolina
Carvalho, Andréa Teixeira de
Pinheiro, Melina de Barros
Lana, Marta de
Martins Filho, Olindo Assis
dc.subject.other.pt_BR.fl_str_mv Doença de chagas
Trypanosoma cruzi
topic Doença de chagas
Trypanosoma cruzi
Chagas disease
Trypanosoma cruzi
dc.subject.en.pt_BR.fl_str_mv Chagas disease
Trypanosoma cruzi
description Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil/ Universidade Federal de São João Del-Rei. Histopatologia e Análises Clínicas. Laboratório CT-Infra II. Divinópolis, MG, Brazil
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-10-20T15:58:37Z
dc.date.available.fl_str_mv 2020-10-20T15:58:37Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv ALESSIO, Glaucia Diniz et al. Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease. Sci Rep, v. 10, 13296, 2020. https://doi.org/10.1038/s41598-020-69921-z
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44081
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-020-69921-z
identifier_str_mv ALESSIO, Glaucia Diniz et al. Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease. Sci Rep, v. 10, 13296, 2020. https://doi.org/10.1038/s41598-020-69921-z
2045-2322
10.1038/s41598-020-69921-z
url https://www.arca.fiocruz.br/handle/icict/44081
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature
publisher.none.fl_str_mv Nature
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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