Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/2768 |
Resumo: | Agradecemos a Lucile Floeter-Winter pela leitura crítica do manuscrito e Artur T.L. de Queiroz para ajuda inicial com a análise filogenética. |
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Oliveira, João Paulo C. deFernandes, Flora Maria de CamposCruz, Angela KayselTrombela, VivianeMonteiro, ElisângelaCamargo, Anamaria ABarral, Aldina Maria PradoOliveira, Camila Indiani de2011-07-25T15:03:47Z2011-07-25T15:03:47Z2007OLIVEIRA, J. P. C. de et al. Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping. Kinetoplastid Biology and Disease, v.6, n.5, p.1-8, 2007.1475-9292https://www.arca.fiocruz.br/handle/icict/276810.1186/1475-9292-6-5Agradecemos a Lucile Floeter-Winter pela leitura crítica do manuscrito e Artur T.L. de Queiroz para ajuda inicial com a análise filogenética.Este trabalho foi financiado pelo CNPq, FAPESB e PAPES/FIOCRUZ.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto, SP, BrasilUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto, SP, BrasilLudwig Institute for Cancer Research. São Paulo, SP, BrasilLudwig Institute for Cancer Research. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilBackground: Leishmania (Leishmania) amazonensis infection in man results in a clinical spectrum of disease manifestations ranging from cutaneous to mucosal or visceral involvement. In the present study, we have investigated the genetic variability of 18 L. amazonensis strains isolated in northeastern Brazil from patients with different clinical manifestations of leishmaniasis. Parasite DNA was analyzed by sequencing of the ITS flanking the 5.8 S subunit of the ribosomal RNA genes, by RAPD and SSR-PCR and by PFGE followed by hybridization with gene-specific probes. Results: ITS sequencing and PCR-based methods revealed genetic heterogeneity among the L. amazonensis isolates examined and molecular karyotyping also showed variation in the chromosome size of different isolates. Unrooted genetic trees separated strains into different groups. Conclusion: These results indicate that L. amazonensis strains isolated from leishmaniasis patients from northeastern Brazil are genetically diverse, however, no correlation between genetic polymorphism and phenotype were found.engDiversidade GenéticaCepasLeishmania amazonensisBrasilNordesteDNASequenciamentoCariotipagem molecularPCRGenetic DiversityStrainsLeishmania amazonensisBrazilNortheasternDNASequencingMolecular karyotypingPCRVariação GenéticaLeishmaniaBrasilAnálise de Sequência de DNACariotipagemReação em Cadeia da PolimeraseGenetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotypinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALOliveira_Fernandes_Cruz_etal.pdfOliveira_Fernandes_Cruz_etal.pdfapplication/pdf332286https://www.arca.fiocruz.br/bitstream/icict/2768/1/Oliveira_Fernandes_Cruz_etal.pdf32e2b037dc817b96564257d4632a65a4MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/2768/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTOliveira_Fernandes_Cruz_etal.pdf.txtOliveira_Fernandes_Cruz_etal.pdf.txtExtracted texttext/plain39442https://www.arca.fiocruz.br/bitstream/icict/2768/5/Oliveira_Fernandes_Cruz_etal.pdf.txt82001409fea8c4fa8b0fee862dd9fbceMD55THUMBNAILOliveira_Fernandes_Cruz_etal.pdf.jpgOliveira_Fernandes_Cruz_etal.pdf.jpgGenerated Thumbnailimage/jpeg2578https://www.arca.fiocruz.br/bitstream/icict/2768/4/Oliveira_Fernandes_Cruz_etal.pdf.jpg60ba9dd1765d95fa2bcc63d59f9b65b0MD54icict/27682023-03-15 14:32:59.735oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:59Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping |
| title |
Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping |
| spellingShingle |
Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping Oliveira, João Paulo C. de Diversidade Genética Cepas Leishmania amazonensis Brasil Nordeste DNA Sequenciamento Cariotipagem molecular PCR Genetic Diversity Strains Leishmania amazonensis Brazil Northeastern DNA Sequencing Molecular karyotyping PCR Variação Genética Leishmania Brasil Análise de Sequência de DNA Cariotipagem Reação em Cadeia da Polimerase |
| title_short |
Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping |
| title_full |
Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping |
| title_fullStr |
Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping |
| title_full_unstemmed |
Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping |
| title_sort |
Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping |
| author |
Oliveira, João Paulo C. de |
| author_facet |
Oliveira, João Paulo C. de Fernandes, Flora Maria de Campos Cruz, Angela Kaysel Trombela, Viviane Monteiro, Elisângela Camargo, Anamaria A Barral, Aldina Maria Prado Oliveira, Camila Indiani de |
| author_role |
author |
| author2 |
Fernandes, Flora Maria de Campos Cruz, Angela Kaysel Trombela, Viviane Monteiro, Elisângela Camargo, Anamaria A Barral, Aldina Maria Prado Oliveira, Camila Indiani de |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Oliveira, João Paulo C. de Fernandes, Flora Maria de Campos Cruz, Angela Kaysel Trombela, Viviane Monteiro, Elisângela Camargo, Anamaria A Barral, Aldina Maria Prado Oliveira, Camila Indiani de |
| dc.subject.other.pt_BR.fl_str_mv |
Diversidade Genética Cepas Leishmania amazonensis Brasil Nordeste DNA Sequenciamento Cariotipagem molecular PCR |
| topic |
Diversidade Genética Cepas Leishmania amazonensis Brasil Nordeste DNA Sequenciamento Cariotipagem molecular PCR Genetic Diversity Strains Leishmania amazonensis Brazil Northeastern DNA Sequencing Molecular karyotyping PCR Variação Genética Leishmania Brasil Análise de Sequência de DNA Cariotipagem Reação em Cadeia da Polimerase |
| dc.subject.en.pt_BR.fl_str_mv |
Genetic Diversity Strains Leishmania amazonensis Brazil Northeastern DNA Sequencing Molecular karyotyping PCR |
| dc.subject.decs.pt_BR.fl_str_mv |
Variação Genética Leishmania Brasil Análise de Sequência de DNA Cariotipagem Reação em Cadeia da Polimerase |
| description |
Agradecemos a Lucile Floeter-Winter pela leitura crítica do manuscrito e Artur T.L. de Queiroz para ajuda inicial com a análise filogenética. |
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2007 |
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2007 |
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2011-07-25T15:03:47Z |
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2011-07-25T15:03:47Z |
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OLIVEIRA, J. P. C. de et al. Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping. Kinetoplastid Biology and Disease, v.6, n.5, p.1-8, 2007. |
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https://www.arca.fiocruz.br/handle/icict/2768 |
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10.1186/1475-9292-6-5 |
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OLIVEIRA, J. P. C. de et al. Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping. Kinetoplastid Biology and Disease, v.6, n.5, p.1-8, 2007. 1475-9292 10.1186/1475-9292-6-5 |
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