Antifungal mechanism of [RuIII(NH3)4catechol]+ complex on fluconazole-resistant Candida tropicalis

Detalhes bibliográficos
Autor(a) principal: Gomes Junior, Rafael Araújo
Data de Publicação: 2017
Outros Autores: Silva, Roberto Santana da, Lima, Renata Galvão de, Santos, Marcos André Vannier dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/23262
Resumo: Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
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spelling Gomes Junior, Rafael AraújoSilva, Roberto Santana daLima, Renata Galvão deSantos, Marcos André Vannier dos2017-11-17T16:34:32Z2017-11-17T16:34:32Z2017GOMES JUNIOR, R. A. et al. Antifungal mechanism of [RuIII(NH3)4catechol]+ complex on fluconazole-resistant Candida tropicalis. FEMS Microbiology Letters, v. 364, n. 9, 20170378-1097https://www.arca.fiocruz.br/handle/icict/2326210.1093/femsle/fnx073engOxford University PressCandida tropicalisResistente ao fluconazolComplexo de rutêniocatecol, agentes antifúngicos, candidíaseCandida tropicalisfluconazole-resistantRuthenium complexcatechol, antifungal agents, candidiasisAntifungal mechanism of [RuIII(NH3)4catechol]+ complex on fluconazole-resistant Candida tropicalisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilUniversidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Ribeirão Preto, SP, BrasilUniversidade Federal de Uberlândia. Faculdade de Ciências Integradas do Pontal. Uberlânida, MG, BrazilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilCandidiasis, a major opportunistic mycosis caused by Candida sp., may comprise life-threatening systemic infections. The incidence of non-albicans species is rising, particularly in South America and they are frequently drug resistant, causing unresponsive cases. Thus, novel antimycotic agents are required. Here we tested the antifungal activity of [RuIII(NH3)4catechol]+ complex (RuCat), approaching possible action mechanisms on fluconazole-resistant Candida tropicalis. RuCat significantly (P < 0.05) inhibited the growth and viability of C. tropicalis dose-dependently (IC50 20.3 μM). Cytotoxicity of RuCat upon murine splenocytes was lower (Selectivity Index = 16). Scanning electron microscopy analysis showed pseudohyphae formation, yeast aggregation and surface damage. RuCat-treated samples investigated by transmission electron microscopy showed melanin granule trafficking to cell surfaces and extracellular milieu. Surface-adherent membrane fragments and extracellular debris were also observed. RuCat treatment produced intense H2DCFDA labeling, indicating reactive oxygen species (ROS) production which caused increased lipoperoxidation. ROS are involved in the fungicidal effect as N-acetyl-L-cysteine completely restored cell viability. Calcofluor White chitin staining suggests that 70 or 140 μM RuCat treatment for 2 h affected cell-wall structure. PI labeling indicated necrotic cell death. The present data indicate that RuCat triggers ROS production, lipoperoxidation and cell surface damage, culminating in selective necrotic death of drug-resistant C. tropicalis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/23262/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGomes Junior RA Fems Microbiol Lett 2017.pdfGomes Junior RA Fems Microbiol Lett 2017.pdfapplication/pdf1391321https://www.arca.fiocruz.br/bitstream/icict/23262/2/Gomes%20Junior%20RA%20Fems%20Microbiol%20Lett%202017.pdfc38ed17a2c495ccf6843a2c475324e30MD52TEXTGomes Junior RA Fems Microbiol Lett 2017.pdf.txtGomes Junior RA Fems Microbiol Lett 2017.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Antifungal mechanism of [RuIII(NH3)4catechol]+ complex on fluconazole-resistant Candida tropicalis
title Antifungal mechanism of [RuIII(NH3)4catechol]+ complex on fluconazole-resistant Candida tropicalis
spellingShingle Antifungal mechanism of [RuIII(NH3)4catechol]+ complex on fluconazole-resistant Candida tropicalis
Gomes Junior, Rafael Araújo
Candida tropicalis
Resistente ao fluconazol
Complexo de rutênio
catecol, agentes antifúngicos, candidíase
Candida tropicalis
fluconazole-resistant
Ruthenium complex
catechol, antifungal agents, candidiasis
title_short Antifungal mechanism of [RuIII(NH3)4catechol]+ complex on fluconazole-resistant Candida tropicalis
title_full Antifungal mechanism of [RuIII(NH3)4catechol]+ complex on fluconazole-resistant Candida tropicalis
title_fullStr Antifungal mechanism of [RuIII(NH3)4catechol]+ complex on fluconazole-resistant Candida tropicalis
title_full_unstemmed Antifungal mechanism of [RuIII(NH3)4catechol]+ complex on fluconazole-resistant Candida tropicalis
title_sort Antifungal mechanism of [RuIII(NH3)4catechol]+ complex on fluconazole-resistant Candida tropicalis
author Gomes Junior, Rafael Araújo
author_facet Gomes Junior, Rafael Araújo
Silva, Roberto Santana da
Lima, Renata Galvão de
Santos, Marcos André Vannier dos
author_role author
author2 Silva, Roberto Santana da
Lima, Renata Galvão de
Santos, Marcos André Vannier dos
author2_role author
author
author
dc.contributor.author.fl_str_mv Gomes Junior, Rafael Araújo
Silva, Roberto Santana da
Lima, Renata Galvão de
Santos, Marcos André Vannier dos
dc.subject.other.pt_BR.fl_str_mv Candida tropicalis
Resistente ao fluconazol
Complexo de rutênio
catecol, agentes antifúngicos, candidíase
topic Candida tropicalis
Resistente ao fluconazol
Complexo de rutênio
catecol, agentes antifúngicos, candidíase
Candida tropicalis
fluconazole-resistant
Ruthenium complex
catechol, antifungal agents, candidiasis
dc.subject.en.pt_BR.fl_str_mv Candida tropicalis
fluconazole-resistant
Ruthenium complex
catechol, antifungal agents, candidiasis
description Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-11-17T16:34:32Z
dc.date.available.fl_str_mv 2017-11-17T16:34:32Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GOMES JUNIOR, R. A. et al. Antifungal mechanism of [RuIII(NH3)4catechol]+ complex on fluconazole-resistant Candida tropicalis. FEMS Microbiology Letters, v. 364, n. 9, 2017
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/23262
dc.identifier.issn.pt_BR.fl_str_mv 0378-1097
dc.identifier.doi.none.fl_str_mv 10.1093/femsle/fnx073
identifier_str_mv GOMES JUNIOR, R. A. et al. Antifungal mechanism of [RuIII(NH3)4catechol]+ complex on fluconazole-resistant Candida tropicalis. FEMS Microbiology Letters, v. 364, n. 9, 2017
0378-1097
10.1093/femsle/fnx073
url https://www.arca.fiocruz.br/handle/icict/23262
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/23262/1/license.txt
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https://www.arca.fiocruz.br/bitstream/icict/23262/3/Gomes%20Junior%20RA%20Fems%20Microbiol%20Lett%202017.pdf.txt
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