The eIF4E subunits of two distinct trypanosomatid eIF4F complexes are subjected to differential post-translational modifications associated to distinct growth phases in culture
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23622 |
Resumo: | O financiamento pessoal aos membros da equipe foi fornecido pelas agências brasileiras de financiamento CNPq , FACEPE e CAPES . O trabalho de laboratório foi parcialmente financiado com subsídios da FACEPE ( APQ-0557-2.02 / 10 ) e os programas PAPES IV e V da FIOCRUZ. A assistência à cultura de parasitas foi fornecida por B. Lima. |
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Pereira, Mariana M. C.Malvezzi, Amaranta M.Nascimento, Larissa M.Lima, Tamara D. C. da CostaAlves, Viviane S.Palma, Mariana L.Freire, Eden R.Moura, Danielle M. N.Reis, Christian R. S.Melo Neto, Osvaldo P. de2017-12-12T13:59:10Z2017-12-12T13:59:10Z2013PEREIRA, M. M. C. et al. The eIF4E subunits of two distinct trypanosomatid eIF4F complexes are subjected to differential post-translational modifications associated to distinct growth phases in culture. Molecular and Biochemical Parasitology, v. 190, n. 2, p. 82–86, ago. 2013.1872-9428https://www.arca.fiocruz.br/handle/icict/2362210.1016/j.molbiopara.2013.06.008O financiamento pessoal aos membros da equipe foi fornecido pelas agências brasileiras de financiamento CNPq , FACEPE e CAPES . O trabalho de laboratório foi parcialmente financiado com subsídios da FACEPE ( APQ-0557-2.02 / 10 ) e os programas PAPES IV e V da FIOCRUZ. A assistência à cultura de parasitas foi fornecida por B. Lima.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.The eukaryotic eIF4F complex, the cap binding complex, functions during translation initiation through interactions mediated by its three subunits (eIF4E, eIF4G and eIF4A), other initiation factors and the ribosome. In trypanosomatids, various eIF4E and eIF4G homologues were identified, with two eIF4F-like complexes confirmed (EIF4E4/EIF4G3/EIF4AI and EIF4E3/EIF4G4/EIF4AI). Here, the expression pattern of these complexes was investigated during Leishmania amazonensis and Trypanosoma brucei growth. The two sets of eIF4E and eIF4G homologues were found represented by phosphorylated isoforms with multiple phosphorylation events targeting the two eIF4E homologues. Expression of these multiple isoforms was differentially affected by inhibitors of mRNA synthesis/processing and translation. Phosphorylated EIF4E4 was consistently associated with early/active growth phases in both organisms studied. In T. brucei phosphorylation of both EIF4E3 and 4, overexpressed as HA-tagged fusions, was partially mapped to their N-terminuses. Our results indicate that phosphorylation is associated with a further layer of complexity in translation initiation in trypanosomatids.engElsevierSíntese proteícaIniciaçãoRegulamentoFosforilaçãoProtein synthesisInitiationRegulationPhosphorylationFator de Iniciação 4E em EucariotosmetabolismoLeishmaniaenzimologiaLeishmaniacrescimento & desenvolvimentoMultimerização ProteicaProcessamento de Proteína Pós-TraducionalTrypanosoma brucei bruceienzimologiaTrypanosoma brucei bruceicrescimento & desenvolvimentoFator de Iniciação 4G em EucariotosmetabolismoPerfilação da Expressão GênicaFosforilaçãoIsoformas de ProteínasmetabolismoSubunidades ProteicasmetabolismoThe eIF4E subunits of two distinct trypanosomatid eIF4F complexes are subjected to differential post-translational modifications associated to distinct growth phases in cultureinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/23622/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALart. 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| dc.title.pt_BR.fl_str_mv |
The eIF4E subunits of two distinct trypanosomatid eIF4F complexes are subjected to differential post-translational modifications associated to distinct growth phases in culture |
| title |
The eIF4E subunits of two distinct trypanosomatid eIF4F complexes are subjected to differential post-translational modifications associated to distinct growth phases in culture |
| spellingShingle |
The eIF4E subunits of two distinct trypanosomatid eIF4F complexes are subjected to differential post-translational modifications associated to distinct growth phases in culture Pereira, Mariana M. C. Síntese proteíca Iniciação Regulamento Fosforilação Protein synthesis Initiation Regulation Phosphorylation Fator de Iniciação 4E em Eucariotos metabolismo Leishmania enzimologia Leishmania crescimento & desenvolvimento Multimerização Proteica Processamento de Proteína Pós-Traducional Trypanosoma brucei brucei enzimologia Trypanosoma brucei brucei crescimento & desenvolvimento Fator de Iniciação 4G em Eucariotos metabolismo Perfilação da Expressão Gênica Fosforilação Isoformas de Proteínas metabolismo Subunidades Proteicas metabolismo |
| title_short |
The eIF4E subunits of two distinct trypanosomatid eIF4F complexes are subjected to differential post-translational modifications associated to distinct growth phases in culture |
| title_full |
The eIF4E subunits of two distinct trypanosomatid eIF4F complexes are subjected to differential post-translational modifications associated to distinct growth phases in culture |
| title_fullStr |
The eIF4E subunits of two distinct trypanosomatid eIF4F complexes are subjected to differential post-translational modifications associated to distinct growth phases in culture |
| title_full_unstemmed |
The eIF4E subunits of two distinct trypanosomatid eIF4F complexes are subjected to differential post-translational modifications associated to distinct growth phases in culture |
| title_sort |
The eIF4E subunits of two distinct trypanosomatid eIF4F complexes are subjected to differential post-translational modifications associated to distinct growth phases in culture |
| author |
Pereira, Mariana M. C. |
| author_facet |
Pereira, Mariana M. C. Malvezzi, Amaranta M. Nascimento, Larissa M. Lima, Tamara D. C. da Costa Alves, Viviane S. Palma, Mariana L. Freire, Eden R. Moura, Danielle M. N. Reis, Christian R. S. Melo Neto, Osvaldo P. de |
| author_role |
author |
| author2 |
Malvezzi, Amaranta M. Nascimento, Larissa M. Lima, Tamara D. C. da Costa Alves, Viviane S. Palma, Mariana L. Freire, Eden R. Moura, Danielle M. N. Reis, Christian R. S. Melo Neto, Osvaldo P. de |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Pereira, Mariana M. C. Malvezzi, Amaranta M. Nascimento, Larissa M. Lima, Tamara D. C. da Costa Alves, Viviane S. Palma, Mariana L. Freire, Eden R. Moura, Danielle M. N. Reis, Christian R. S. Melo Neto, Osvaldo P. de |
| dc.subject.other.pt_BR.fl_str_mv |
Síntese proteíca Iniciação Regulamento Fosforilação |
| topic |
Síntese proteíca Iniciação Regulamento Fosforilação Protein synthesis Initiation Regulation Phosphorylation Fator de Iniciação 4E em Eucariotos metabolismo Leishmania enzimologia Leishmania crescimento & desenvolvimento Multimerização Proteica Processamento de Proteína Pós-Traducional Trypanosoma brucei brucei enzimologia Trypanosoma brucei brucei crescimento & desenvolvimento Fator de Iniciação 4G em Eucariotos metabolismo Perfilação da Expressão Gênica Fosforilação Isoformas de Proteínas metabolismo Subunidades Proteicas metabolismo |
| dc.subject.en.pt_BR.fl_str_mv |
Protein synthesis Initiation Regulation Phosphorylation |
| dc.subject.decs.pt_BR.fl_str_mv |
Fator de Iniciação 4E em Eucariotos metabolismo Leishmania enzimologia Leishmania crescimento & desenvolvimento Multimerização Proteica Processamento de Proteína Pós-Traducional Trypanosoma brucei brucei enzimologia Trypanosoma brucei brucei crescimento & desenvolvimento Fator de Iniciação 4G em Eucariotos metabolismo Perfilação da Expressão Gênica Fosforilação Isoformas de Proteínas metabolismo Subunidades Proteicas metabolismo |
| description |
O financiamento pessoal aos membros da equipe foi fornecido pelas agências brasileiras de financiamento CNPq , FACEPE e CAPES . O trabalho de laboratório foi parcialmente financiado com subsídios da FACEPE ( APQ-0557-2.02 / 10 ) e os programas PAPES IV e V da FIOCRUZ. A assistência à cultura de parasitas foi fornecida por B. Lima. |
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2013 |
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2013 |
| dc.date.accessioned.fl_str_mv |
2017-12-12T13:59:10Z |
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2017-12-12T13:59:10Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
PEREIRA, M. M. C. et al. The eIF4E subunits of two distinct trypanosomatid eIF4F complexes are subjected to differential post-translational modifications associated to distinct growth phases in culture. Molecular and Biochemical Parasitology, v. 190, n. 2, p. 82–86, ago. 2013. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/23622 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1872-9428 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.molbiopara.2013.06.008 |
| identifier_str_mv |
PEREIRA, M. M. C. et al. The eIF4E subunits of two distinct trypanosomatid eIF4F complexes are subjected to differential post-translational modifications associated to distinct growth phases in culture. Molecular and Biochemical Parasitology, v. 190, n. 2, p. 82–86, ago. 2013. 1872-9428 10.1016/j.molbiopara.2013.06.008 |
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https://www.arca.fiocruz.br/handle/icict/23622 |
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eng |
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eng |
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openAccess |
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Elsevier |
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Elsevier |
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