Synergistic antileishmanial effect of oregano essential oil and silver nanoparticles: mechanisms of action on Leishmania amazonensis
| Autor(a) principal: | |
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| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/59223 https://doi.org/10.3390/ pathogens12050660 |
Resumo: | Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunoparasitologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil. |
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Alves, Alex BarbosaBortoleti, Bruna Taciane da SilvaTomiotto-Pellissier, FernandaGanaza, Ana Flávia MarquesGonçalves, Manoela DaieleCarloto, Amanda Cristina MachadoRodrigues, Ana Carolina JacobSilva, Taylon FelipeNakazato, GersonKobayashi, Renata Katsuko TakayamaLazarin-Bidóia, DanielleMiranda-Sapla, Milena MenegazzoCosta, Idessania NazarethPavanelli, Wander RogérioConchon-Costa, Ivete2023-06-28T16:01:56Z2023-06-28T16:01:56Z2023ALVES, Alex Barbosa et al. Synergistic antileishmanial effect of oregano essential oil and silver nanoparticles: mechanisms of action on Leishmania amazonensis. Pathogens, v. 12, n. 660, p. 1-17, 2023.2076-0817https://www.arca.fiocruz.br/handle/icict/59223https://doi.org/10.3390/ pathogens12050660porMDPIOriganumLeishmaniasisPlant OilsApoptosisAntileishmaniasisAceites de PlantasHuiles végétalesLeishmanioseOrégano (Planta)Óleos de PlantasApoptoseAntileishmanioseSynergistic antileishmanial effect of oregano essential oil and silver nanoparticles: mechanisms of action on Leishmania amazonensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunoparasitologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunoparasitologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunoparasitologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunoparasitologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Exatas. Departamento de Química. Laboratório de Biotransformação e Fitoquímica. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunoparasitologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunoparasitologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunoparasitologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Microbiologia. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Microbiologia. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunoparasitologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunoparasitologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunoparasitologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunoparasitologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunoparasitologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil.American tegumentary leishmaniasis, a zoonotic disease caused by the Leishmania genus, poses significant challenges in treatment, including administration difficulty, low efficacy, and parasite resistance. Novel compounds or associations offer alternative therapies, and natural products such as oregano essential oil (OEO), extracted from Origanum vulgare, have been extensively researched due to biological effects, including antibacterial, antifungal, and antiparasitic properties. Silver nanoparticles (AgNp), a nanomaterial with compelling antimicrobial and antiparasitic activity, have been shown to exhibit potent leishmanicidal properties. We evaluated the in vitro effect of OEO and AgNp-Bio association on L. amazonensis and the death mechanisms of the parasite involved. Our results demonstrated a synergistic antileishmanial effect of OEO + AgNp on promastigote forms and L. amazonensis-infected macrophages, which induced morphological and ultrastructural changes in promastigotes. Subsequently, we investigated the mechanisms underlying parasite death and showed an increase in NO, ROS, mitochondrial depolarization, accumulation of lipid-storage bodies, autophagic vacuoles, phosphatidylserine exposure, and damage to the plasma membrane. Moreover, the association resulted in a reduction in the percentage of infected cells and the number of amastigotes per macrophage. In conclusion, our findings establish that OEO + AgNp elicits a late apoptosis-like mechanism to combat promastigote forms and promotes ROS and NO production in infected macrophages to target intracellular amastigote forms.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/59223/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALpathogens-12-00660OK.pdfpathogens-12-00660OK.pdfapplication/pdf3239344https://www.arca.fiocruz.br/bitstream/icict/59223/2/pathogens-12-00660OK.pdf31441cf215bfdc6cfde0e2a7ae74d2f3MD52icict/592232023-06-28 13:01:57.236oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-06-28T16:01:57Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en_US.fl_str_mv |
Synergistic antileishmanial effect of oregano essential oil and silver nanoparticles: mechanisms of action on Leishmania amazonensis |
| title |
Synergistic antileishmanial effect of oregano essential oil and silver nanoparticles: mechanisms of action on Leishmania amazonensis |
| spellingShingle |
Synergistic antileishmanial effect of oregano essential oil and silver nanoparticles: mechanisms of action on Leishmania amazonensis Alves, Alex Barbosa Origanum Leishmaniasis Plant Oils Apoptosis Antileishmaniasis Aceites de Plantas Huiles végétales Leishmaniose Orégano (Planta) Óleos de Plantas Apoptose Antileishmaniose |
| title_short |
Synergistic antileishmanial effect of oregano essential oil and silver nanoparticles: mechanisms of action on Leishmania amazonensis |
| title_full |
Synergistic antileishmanial effect of oregano essential oil and silver nanoparticles: mechanisms of action on Leishmania amazonensis |
| title_fullStr |
Synergistic antileishmanial effect of oregano essential oil and silver nanoparticles: mechanisms of action on Leishmania amazonensis |
| title_full_unstemmed |
Synergistic antileishmanial effect of oregano essential oil and silver nanoparticles: mechanisms of action on Leishmania amazonensis |
| title_sort |
Synergistic antileishmanial effect of oregano essential oil and silver nanoparticles: mechanisms of action on Leishmania amazonensis |
| author |
Alves, Alex Barbosa |
| author_facet |
Alves, Alex Barbosa Bortoleti, Bruna Taciane da Silva Tomiotto-Pellissier, Fernanda Ganaza, Ana Flávia Marques Gonçalves, Manoela Daiele Carloto, Amanda Cristina Machado Rodrigues, Ana Carolina Jacob Silva, Taylon Felipe Nakazato, Gerson Kobayashi, Renata Katsuko Takayama Lazarin-Bidóia, Danielle Miranda-Sapla, Milena Menegazzo Costa, Idessania Nazareth Pavanelli, Wander Rogério Conchon-Costa, Ivete |
| author_role |
author |
| author2 |
Bortoleti, Bruna Taciane da Silva Tomiotto-Pellissier, Fernanda Ganaza, Ana Flávia Marques Gonçalves, Manoela Daiele Carloto, Amanda Cristina Machado Rodrigues, Ana Carolina Jacob Silva, Taylon Felipe Nakazato, Gerson Kobayashi, Renata Katsuko Takayama Lazarin-Bidóia, Danielle Miranda-Sapla, Milena Menegazzo Costa, Idessania Nazareth Pavanelli, Wander Rogério Conchon-Costa, Ivete |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Alves, Alex Barbosa Bortoleti, Bruna Taciane da Silva Tomiotto-Pellissier, Fernanda Ganaza, Ana Flávia Marques Gonçalves, Manoela Daiele Carloto, Amanda Cristina Machado Rodrigues, Ana Carolina Jacob Silva, Taylon Felipe Nakazato, Gerson Kobayashi, Renata Katsuko Takayama Lazarin-Bidóia, Danielle Miranda-Sapla, Milena Menegazzo Costa, Idessania Nazareth Pavanelli, Wander Rogério Conchon-Costa, Ivete |
| dc.subject.other.en_US.fl_str_mv |
Origanum |
| topic |
Origanum Leishmaniasis Plant Oils Apoptosis Antileishmaniasis Aceites de Plantas Huiles végétales Leishmaniose Orégano (Planta) Óleos de Plantas Apoptose Antileishmaniose |
| dc.subject.en.en_US.fl_str_mv |
Leishmaniasis Plant Oils Apoptosis Antileishmaniasis |
| dc.subject.es.en_US.fl_str_mv |
Aceites de Plantas |
| dc.subject.fr.en_US.fl_str_mv |
Huiles végétales |
| dc.subject.decs.en_US.fl_str_mv |
Leishmaniose Orégano (Planta) Óleos de Plantas Apoptose Antileishmaniose |
| description |
Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Laboratório de Imunoparasitologia de Doenças Negligenciadas e Câncer. Londrina, PR, Brasil. |
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2023 |
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2023-06-28T16:01:56Z |
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2023-06-28T16:01:56Z |
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2023 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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ALVES, Alex Barbosa et al. Synergistic antileishmanial effect of oregano essential oil and silver nanoparticles: mechanisms of action on Leishmania amazonensis. Pathogens, v. 12, n. 660, p. 1-17, 2023. |
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https://www.arca.fiocruz.br/handle/icict/59223 |
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2076-0817 |
| dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.3390/ pathogens12050660 |
| identifier_str_mv |
ALVES, Alex Barbosa et al. Synergistic antileishmanial effect of oregano essential oil and silver nanoparticles: mechanisms of action on Leishmania amazonensis. Pathogens, v. 12, n. 660, p. 1-17, 2023. 2076-0817 |
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https://www.arca.fiocruz.br/handle/icict/59223 https://doi.org/10.3390/ pathogens12050660 |
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MDPI |
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MDPI |
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