Multiplex Nucleic Acid Amplification Test for Diagnosis of Dengue Fever, Malaria, and Leptospirosis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/10870 |
Resumo: | Stanford University School of Medicine. Department of Medicine. Division of Infectious Diseases and Geographic Medicine. Stanford, CA, USA. |
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Waggoner, Jesse JAbeynayake, JanakiBalassiano, IlanaLefterova, MartinaSahoo, Malaya KLiu, YuanyuanVital-Brazil, Juliana MagalhãesGresh, LionelBalmaseda, AngelHarris, EvaBanael, NiazPinsky, Benjamin A2015-06-17T12:05:21Z2015-06-17T12:05:21Z2014WAGGONER, Jesse J. et al. Multiplex Nucleic Acid Amplification Test for Diagnosis of Dengue Fever, Malaria, and Leptospirosis. Journal of Clinical Microbiology, v.52, n.6, p.2011-2018, jun. 2014.1098-660Xhttps://www.arca.fiocruz.br/handle/icict/1087010.1128/JCM.00341-14engPubMed CommonsMultiplex Nucleic Acid Amplification Test for Diagnosis of Dengue Fever, Malaria, and Leptospirosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleStanford University School of Medicine. Department of Medicine. Division of Infectious Diseases and Geographic Medicine. Stanford, CA, USA.Stanford University School of Medicine. Department of Pathology. Stanford, CA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. WHO/PAHO Centro Colaborador para Leptospirose. Centro de Referência Nacional para Leptospirose. Coleção de Leptospira. Rio de Janeiro, RJ, Brasil.Stanford University School of Medicine. Department of Pathology. Stanford, CA, USA.Stanford University School of Medicine. Department of Pathology. Stanford, CA, USA.Stanford University School of Medicine. Department of Pathology. Stanford, CA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. WHO/PAHO Centro Colaborador para Leptospirose. Centro de Referência Nacional para Leptospirose. Coleção de Leptospira. Rio de Janeiro, RJ, Brasil.Sustainable Sciences Institute. Managua, Nicaragua.Ministry of Health. Centro Nacional de Diagnóstico y Referencia. National Virology Laboratory. Mnagua, Nicaragua.University of California. School of Public Health. Division of Infectious Diseases and Vaccinology. Berkeley, CA, USA.Dengue, leptospirosis, and malaria are among the most common etiologies of systemic undifferentiated febrile illness (UFI) among travelers to the developing world, and these pathogens all have the potential to cause life-threatening illness in returned travelers. The current study describes the development of an internally controlled multiplex nucleic acid amplification test for the detection of dengue virus (DENV) and Leptospira and Plasmodium species, with a specific callout for Plasmodium falciparum (referred to as the UFI assay). During analytical evaluation, the UFI assay displayed a wide dynamic range and a sensitive limit of detection for each target, including all four DENV serotypes. In a clinical evaluation including 210 previously tested samples, the sensitivities of the UFI assay were 98% for DENV (58/59 samples detected) and 100% for Leptospira and malaria (65/65 and 20/20 samples, respectively). Malaria samples included all five Plasmodium species known to cause human disease. The specificity of the UFI assay was 100% when evaluated with a panel of 66 negative clinical samples. Furthermore, no amplification was observed when extracted nucleic acids from related pathogens were tested. Compared with whole-blood samples, the UFI assay remained positive for Plasmodium in 11 plasma samples from patients with malaria (parasitemia levels of 0.0037 to 3.4%). The syndrome-based design of the UFI assay, combined with the sensitivities of the component tests, represents a significant improvement over the individual diagnostic tests available for these pathogens.Dengue feverMalariaLeptospirosisNucleic AcidÁcidos NucleicosDengueMaláriaLeptospiroseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10870/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALilana_balassianoetal_IOC_2014.pdfapplication/pdf255699https://www.arca.fiocruz.br/bitstream/icict/10870/2/ilana_balassianoetal_IOC_2014.pdf3d36819ae7364a9491227409490d48e9MD52TEXTilana_balassianoetal_IOC_2014.pdf.txtilana_balassianoetal_IOC_2014.pdf.txtExtracted texttext/plain51140https://www.arca.fiocruz.br/bitstream/icict/10870/3/ilana_balassianoetal_IOC_2014.pdf.txt0959207475b37b65d5e3fa3d4a772027MD53icict/108702022-06-24 13:12:38.05oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:12:38Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Multiplex Nucleic Acid Amplification Test for Diagnosis of Dengue Fever, Malaria, and Leptospirosis |
| title |
Multiplex Nucleic Acid Amplification Test for Diagnosis of Dengue Fever, Malaria, and Leptospirosis |
| spellingShingle |
Multiplex Nucleic Acid Amplification Test for Diagnosis of Dengue Fever, Malaria, and Leptospirosis Waggoner, Jesse J Dengue fever Malaria Leptospirosis Nucleic Acid Ácidos Nucleicos Dengue Malária Leptospirose |
| title_short |
Multiplex Nucleic Acid Amplification Test for Diagnosis of Dengue Fever, Malaria, and Leptospirosis |
| title_full |
Multiplex Nucleic Acid Amplification Test for Diagnosis of Dengue Fever, Malaria, and Leptospirosis |
| title_fullStr |
Multiplex Nucleic Acid Amplification Test for Diagnosis of Dengue Fever, Malaria, and Leptospirosis |
| title_full_unstemmed |
Multiplex Nucleic Acid Amplification Test for Diagnosis of Dengue Fever, Malaria, and Leptospirosis |
| title_sort |
Multiplex Nucleic Acid Amplification Test for Diagnosis of Dengue Fever, Malaria, and Leptospirosis |
| author |
Waggoner, Jesse J |
| author_facet |
Waggoner, Jesse J Abeynayake, Janaki Balassiano, Ilana Lefterova, Martina Sahoo, Malaya K Liu, Yuanyuan Vital-Brazil, Juliana Magalhães Gresh, Lionel Balmaseda, Angel Harris, Eva Banael, Niaz Pinsky, Benjamin A |
| author_role |
author |
| author2 |
Abeynayake, Janaki Balassiano, Ilana Lefterova, Martina Sahoo, Malaya K Liu, Yuanyuan Vital-Brazil, Juliana Magalhães Gresh, Lionel Balmaseda, Angel Harris, Eva Banael, Niaz Pinsky, Benjamin A |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Waggoner, Jesse J Abeynayake, Janaki Balassiano, Ilana Lefterova, Martina Sahoo, Malaya K Liu, Yuanyuan Vital-Brazil, Juliana Magalhães Gresh, Lionel Balmaseda, Angel Harris, Eva Banael, Niaz Pinsky, Benjamin A |
| dc.subject.en.pt_BR.fl_str_mv |
Dengue fever Malaria Leptospirosis Nucleic Acid |
| topic |
Dengue fever Malaria Leptospirosis Nucleic Acid Ácidos Nucleicos Dengue Malária Leptospirose |
| dc.subject.decs.pt_BR.fl_str_mv |
Ácidos Nucleicos Dengue Malária Leptospirose |
| description |
Stanford University School of Medicine. Department of Medicine. Division of Infectious Diseases and Geographic Medicine. Stanford, CA, USA. |
| publishDate |
2014 |
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2014 |
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2015-06-17T12:05:21Z |
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2015-06-17T12:05:21Z |
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info:eu-repo/semantics/article |
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WAGGONER, Jesse J. et al. Multiplex Nucleic Acid Amplification Test for Diagnosis of Dengue Fever, Malaria, and Leptospirosis. Journal of Clinical Microbiology, v.52, n.6, p.2011-2018, jun. 2014. |
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https://www.arca.fiocruz.br/handle/icict/10870 |
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1098-660X |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.1128/JCM.00341-14 |
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WAGGONER, Jesse J. et al. Multiplex Nucleic Acid Amplification Test for Diagnosis of Dengue Fever, Malaria, and Leptospirosis. Journal of Clinical Microbiology, v.52, n.6, p.2011-2018, jun. 2014. 1098-660X 10.1128/JCM.00341-14 |
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