Microscopic detection of Mycobacterium tuberculosis in direct or processed sputum smears

Detalhes bibliográficos
Autor(a) principal: Magalhães, José Luiz de Oliveira
Data de Publicação: 2018
Outros Autores: Lima, Juliana Figueiredo da Costa, Araújo, Ana Albertina de, Coutinho, Ilyana Oliveira, Leal, Nilma Cintra, Almeida, Alzira Maria Paiva de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32822
Resumo: Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.
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spelling Magalhães, José Luiz de OliveiraLima, Juliana Figueiredo da CostaAraújo, Ana Albertina deCoutinho, Ilyana OliveiraLeal, Nilma CintraAlmeida, Alzira Maria Paiva de2019-04-29T13:11:17Z2019-04-29T13:11:17Z20181678-9849https://www.arca.fiocruz.br/handle/icict/3282210.1590/0037-8682-0238-2017engMicroscopic detection of Mycobacterium tuberculosis in direct or processed sputum smearsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Laboratório Municipal de Saúde Pública. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.Introduction: Microscopic identification of active pulmonary tuberculosis (PTB) from direct smears of sputum (DS) is widely used for detection, but has limited sensitivity. Here, we assessed the yield of acid-fast bacilli (AFB) detection in processed sputum smears (PSS). Methods: Sputum samples were simultaneously analyzed by direct sputum smearing and after chemical treatment and spontaneous sedimentation. Results: Of the 1,719 samples analyzed, 16.4% were positive for AFB in conventional DS and 21.4% in PSS, corresponding to a 30% increase in detection. Conclusions: Increased sensitivity from analyzing PSS and better safety protocols will contribute to improved detection and control of the disease.TuberculosisSputum processedSmear microscopyHumanosMycobacterium tuberculosis / isolamento & purificaçãoSensibilidade e EspecificidadeManipulação de Amostras / métodosMutação / microbiologiaTuberculose Pulmonar / diagnósticoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83094https://www.arca.fiocruz.br/bitstream/icict/32822/1/license.txtee3692f7b0b97d5638099ab94397d05fMD51ORIGINALMicroscopic detection of Mycobacterium tuberculosis in direct or processed sputum smears.pdfMicroscopic detection of Mycobacterium tuberculosis in direct or processed sputum 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dc.title.pt_BR.fl_str_mv Microscopic detection of Mycobacterium tuberculosis in direct or processed sputum smears
title Microscopic detection of Mycobacterium tuberculosis in direct or processed sputum smears
spellingShingle Microscopic detection of Mycobacterium tuberculosis in direct or processed sputum smears
Magalhães, José Luiz de Oliveira
Tuberculosis
Sputum processed
Smear microscopy
Humanos
Mycobacterium tuberculosis / isolamento & purificação
Sensibilidade e Especificidade
Manipulação de Amostras / métodos
Mutação / microbiologia
Tuberculose Pulmonar / diagnóstico
title_short Microscopic detection of Mycobacterium tuberculosis in direct or processed sputum smears
title_full Microscopic detection of Mycobacterium tuberculosis in direct or processed sputum smears
title_fullStr Microscopic detection of Mycobacterium tuberculosis in direct or processed sputum smears
title_full_unstemmed Microscopic detection of Mycobacterium tuberculosis in direct or processed sputum smears
title_sort Microscopic detection of Mycobacterium tuberculosis in direct or processed sputum smears
author Magalhães, José Luiz de Oliveira
author_facet Magalhães, José Luiz de Oliveira
Lima, Juliana Figueiredo da Costa
Araújo, Ana Albertina de
Coutinho, Ilyana Oliveira
Leal, Nilma Cintra
Almeida, Alzira Maria Paiva de
author_role author
author2 Lima, Juliana Figueiredo da Costa
Araújo, Ana Albertina de
Coutinho, Ilyana Oliveira
Leal, Nilma Cintra
Almeida, Alzira Maria Paiva de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Magalhães, José Luiz de Oliveira
Lima, Juliana Figueiredo da Costa
Araújo, Ana Albertina de
Coutinho, Ilyana Oliveira
Leal, Nilma Cintra
Almeida, Alzira Maria Paiva de
dc.subject.en.pt_BR.fl_str_mv Tuberculosis
Sputum processed
Smear microscopy
topic Tuberculosis
Sputum processed
Smear microscopy
Humanos
Mycobacterium tuberculosis / isolamento & purificação
Sensibilidade e Especificidade
Manipulação de Amostras / métodos
Mutação / microbiologia
Tuberculose Pulmonar / diagnóstico
dc.subject.decs.pt_BR.fl_str_mv Humanos
Mycobacterium tuberculosis / isolamento & purificação
Sensibilidade e Especificidade
Manipulação de Amostras / métodos
Mutação / microbiologia
Tuberculose Pulmonar / diagnóstico
description Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-04-29T13:11:17Z
dc.date.available.fl_str_mv 2019-04-29T13:11:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32822
dc.identifier.issn.pt_BR.fl_str_mv 1678-9849
dc.identifier.doi.none.fl_str_mv 10.1590/0037-8682-0238-2017
identifier_str_mv 1678-9849
10.1590/0037-8682-0238-2017
url https://www.arca.fiocruz.br/handle/icict/32822
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/32822/1/license.txt
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