An inhibition ELISA to determine alpha macroglobulin levels in mouse plasma

Detalhes bibliográficos
Autor(a) principal: Luz, Mauricio R. M. P.
Data de Publicação: 1994
Outros Autores: Van Leuven, Fred, Araujo Jorge, Tania C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36854
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil.
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spelling Luz, Mauricio R. M. P.Van Leuven, FredAraujo Jorge, Tania C.2019-11-05T12:08:20Z2019-11-05T12:08:20Z1994LUZ, Mauricio R. M. P.; VAN LEUVEN, Fred; ARAUJO JORGE, Tania C. An inhibition ELISA to determine α macroglobulin levels in mouse plasma. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 89, n. 2, p. 203-207, Apr./June 1994.0074-0276https://www.arca.fiocruz.br/handle/icict/3685410.1590/s0074-027619940002000141678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzMurinoglobulinaELISAα-2-macroglobulinA2MELISAMurinoglobulinAn inhibition ELISA to determine alpha macroglobulin levels in mouse plasmainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil.Katholic University of Leuven. Dept. of Human Genetics. Leuven, Belgium.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil.A sensitive method for quantifying mouse plasma alpha-macroglobulins (AM) using an inhibition ELISA is described. AM are important plasma proteinase inhibitors that possibly act also as immunomodulatory molecules. The standard protocol developed in our experiments involves coating well with 10 micrograms/ml A2M in carbonate buffer, followed by incubation with a 1:1 (v/v) mixture of the plasma to be tested (diluted 1/1000) and goat anti-AM (diluted 1/1250). This is followed by further incubation, first with the enzyme-conjugated antibody and with the substrate prior to the reading of absorbance levels of the reaction products. Standard curve samples must be included in each plate, employing known amounts of the purified Murine Alpha-2-Macroglobulin (MuA2M) used for coating, with concentrations ranging from 0.001 to 10 micrograms/ml. Using test samples in triplicates and a 6-point standard curve in a single ELISA plate, 25 plasma samples can be tested accurately. 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dc.title.pt_BR.fl_str_mv An inhibition ELISA to determine alpha macroglobulin levels in mouse plasma
title An inhibition ELISA to determine alpha macroglobulin levels in mouse plasma
spellingShingle An inhibition ELISA to determine alpha macroglobulin levels in mouse plasma
Luz, Mauricio R. M. P.
Murinoglobulina
ELISA
α-2-macroglobulin
A2M
ELISA
Murinoglobulin
title_short An inhibition ELISA to determine alpha macroglobulin levels in mouse plasma
title_full An inhibition ELISA to determine alpha macroglobulin levels in mouse plasma
title_fullStr An inhibition ELISA to determine alpha macroglobulin levels in mouse plasma
title_full_unstemmed An inhibition ELISA to determine alpha macroglobulin levels in mouse plasma
title_sort An inhibition ELISA to determine alpha macroglobulin levels in mouse plasma
author Luz, Mauricio R. M. P.
author_facet Luz, Mauricio R. M. P.
Van Leuven, Fred
Araujo Jorge, Tania C.
author_role author
author2 Van Leuven, Fred
Araujo Jorge, Tania C.
author2_role author
author
dc.contributor.author.fl_str_mv Luz, Mauricio R. M. P.
Van Leuven, Fred
Araujo Jorge, Tania C.
dc.subject.other.pt_BR.fl_str_mv Murinoglobulina
ELISA
topic Murinoglobulina
ELISA
α-2-macroglobulin
A2M
ELISA
Murinoglobulin
dc.subject.en.pt_BR.fl_str_mv α-2-macroglobulin
A2M
ELISA
Murinoglobulin
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil.
publishDate 1994
dc.date.issued.fl_str_mv 1994
dc.date.accessioned.fl_str_mv 2019-11-05T12:08:20Z
dc.date.available.fl_str_mv 2019-11-05T12:08:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LUZ, Mauricio R. M. P.; VAN LEUVEN, Fred; ARAUJO JORGE, Tania C. An inhibition ELISA to determine α macroglobulin levels in mouse plasma. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 89, n. 2, p. 203-207, Apr./June 1994.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36854
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/s0074-02761994000200014
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv LUZ, Mauricio R. M. P.; VAN LEUVEN, Fred; ARAUJO JORGE, Tania C. An inhibition ELISA to determine α macroglobulin levels in mouse plasma. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 89, n. 2, p. 203-207, Apr./June 1994.
0074-0276
10.1590/s0074-02761994000200014
1678-8060
url https://www.arca.fiocruz.br/handle/icict/36854
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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