Infection of mouse neural progenitor cells by Toxoplasma gondii affects in vitro proliferation, differentiation and migration

Detalhes bibliográficos
Autor(a) principal: Pires, Luiza Bendia
Data de Publicação: 2021
Outros Autores: Barbosa, Helene Santos, Santiago, Marcelo Felippe, Adesse, Daniel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/48121
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Neurobiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil.
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spelling Pires, Luiza BendiaBarbosa, Helene SantosSantiago, Marcelo FelippeAdesse, Daniel2021-07-12T12:50:40Z2021-07-12T12:50:40Z2021PIRES, Luiza Bendia et al. Infection of mouse neural progenitor cells by Toxoplasma gondii affects in vitro proliferation, differentiation and migration. bioRxiv, p. 1-46, June 2021.https://www.arca.fiocruz.br/handle/icict/4812110.1101/2021.05.31.446482engbioRxivToxoplasmose congênitaNeurogêneseCélulas progenitoras neuraisNeuroferasToxoplasma gondiiGliogêneseComplexo torchCongenital toxoplasmosisNeurogenesisNeural progenitor cellsNeurospheresToxoplasma gondiiGliogenesisTORCH complexInfection of mouse neural progenitor cells by Toxoplasma gondii affects in vitro proliferation, differentiation and migrationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Neurobiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Neurobiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, BrasilCongenital toxoplasmosis constitutes a major cause of pre- and post-natal complications. Fetal infection with Toxoplasma gondii influences development and can lead to microcephaly, encephalitis, and neurological abnormalities. Few studies have attempted to explain the impact of T. gondii infection on the physiology of mature nerve cells, and no systematic study concerning the effect of infection of neural progenitor cells by T. gondii in the biology of these progenitors is available. We infected cortical intermediate progenitor cell cultivated as neurospheres obtained from E16.5 Swiss Webster mice with T. gondii (Me49 strain) tachyzoites to mimic the developing mouse cerebral cortex in vitro. Infection decreased cell proliferation as detected by Ki67 staining at 48 and 72 hours post infection (hpi) in floating neurospheres, resulting in reduced cellularity at 96 hpi. Neurogenic and gliogenic potential, assessed in plated neurospheres, was shown to be impaired in infected cultures, as indicated by neurofilament heavy chain (NF-200) and GFAP staining, respectively. To further investigate the impact of infection on neuronal differentiation, Neuro2a neuroblasts were infected and after 24 hpi, neurogenic differentiation was induced with serum withdrawal. We confirmed that infection induces a decrease in neuroblast-neuron differentiation rates in cells stained for NF-200, with reduced neuritogenesis. Migration rates were analyzed in plated neurospheres. At 120 h after plating, infected cultures exhibited decreased overall migration rates and altered the radial migration of nestin-, GFAP- and NF-200- positive cells. These findings indicate that T. gondii infection of neural progenitor cells may lead to reduced neuro/gliogenesis due to an imbalance in cell proliferation alongside an altered migratory profile. If translated to the in vivo situation, these data could explain, in part, the cortical malformations observed in congenitally infected individuals.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/48121/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLuisaBPires_DanielAdesse_ètal_prep_IOC_2021.pdfLuisaBPires_DanielAdesse_ètal_prep_IOC_2021.pdfapplication/pdf3837323https://www.arca.fiocruz.br/bitstream/icict/48121/2/LuisaBPires_DanielAdesse_%c3%a8tal_prep_IOC_2021.pdf1195a6f9e3d492610e1a1d6b54747a3aMD52TEXTLuisaBPires_DanielAdesse_ètal_prep_IOC_2021.pdf.txtLuisaBPires_DanielAdesse_ètal_prep_IOC_2021.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Infection of mouse neural progenitor cells by Toxoplasma gondii affects in vitro proliferation, differentiation and migration
title Infection of mouse neural progenitor cells by Toxoplasma gondii affects in vitro proliferation, differentiation and migration
spellingShingle Infection of mouse neural progenitor cells by Toxoplasma gondii affects in vitro proliferation, differentiation and migration
Pires, Luiza Bendia
Toxoplasmose congênita
Neurogênese
Células progenitoras neurais
Neuroferas
Toxoplasma gondii
Gliogênese
Complexo torch
Congenital toxoplasmosis
Neurogenesis
Neural progenitor cells
Neurospheres
Toxoplasma gondii
Gliogenesis
TORCH complex
title_short Infection of mouse neural progenitor cells by Toxoplasma gondii affects in vitro proliferation, differentiation and migration
title_full Infection of mouse neural progenitor cells by Toxoplasma gondii affects in vitro proliferation, differentiation and migration
title_fullStr Infection of mouse neural progenitor cells by Toxoplasma gondii affects in vitro proliferation, differentiation and migration
title_full_unstemmed Infection of mouse neural progenitor cells by Toxoplasma gondii affects in vitro proliferation, differentiation and migration
title_sort Infection of mouse neural progenitor cells by Toxoplasma gondii affects in vitro proliferation, differentiation and migration
author Pires, Luiza Bendia
author_facet Pires, Luiza Bendia
Barbosa, Helene Santos
Santiago, Marcelo Felippe
Adesse, Daniel
author_role author
author2 Barbosa, Helene Santos
Santiago, Marcelo Felippe
Adesse, Daniel
author2_role author
author
author
dc.contributor.author.fl_str_mv Pires, Luiza Bendia
Barbosa, Helene Santos
Santiago, Marcelo Felippe
Adesse, Daniel
dc.subject.other.pt_BR.fl_str_mv Toxoplasmose congênita
Neurogênese
Células progenitoras neurais
Neuroferas
Toxoplasma gondii
Gliogênese
Complexo torch
topic Toxoplasmose congênita
Neurogênese
Células progenitoras neurais
Neuroferas
Toxoplasma gondii
Gliogênese
Complexo torch
Congenital toxoplasmosis
Neurogenesis
Neural progenitor cells
Neurospheres
Toxoplasma gondii
Gliogenesis
TORCH complex
dc.subject.en.pt_BR.fl_str_mv Congenital toxoplasmosis
Neurogenesis
Neural progenitor cells
Neurospheres
Toxoplasma gondii
Gliogenesis
TORCH complex
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Neurobiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-07-12T12:50:40Z
dc.date.available.fl_str_mv 2021-07-12T12:50:40Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PIRES, Luiza Bendia et al. Infection of mouse neural progenitor cells by Toxoplasma gondii affects in vitro proliferation, differentiation and migration. bioRxiv, p. 1-46, June 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/48121
dc.identifier.doi.pt_BR.fl_str_mv 10.1101/2021.05.31.446482
identifier_str_mv PIRES, Luiza Bendia et al. Infection of mouse neural progenitor cells by Toxoplasma gondii affects in vitro proliferation, differentiation and migration. bioRxiv, p. 1-46, June 2021.
10.1101/2021.05.31.446482
url https://www.arca.fiocruz.br/handle/icict/48121
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv bioRxiv
publisher.none.fl_str_mv bioRxiv
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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