A Predicted Mannoprotein Participates in Cryptococcus gattii Capsular Structure
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/30985 |
Resumo: | Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Laboratório de Fungos de Importância Médica e Biotecnológica. Porto Alegre, RS, Brasil. |
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Reuwsaat, Julia Catarina VieiraMotta, HerykGarcia, Ane Wichine AcostaVasconcelos, Carolina BettkerMarques, Bárbara MachadoOliveira, Natália KronbauerRodrigues, JéssicaFerrareze, Patrícia Aline GröhnsFrases, SusanaLopes, WilliamBarcellos, Vanessa AbreuSquizani, Eamim DaidrêHorta, Jorge AndréSchrank, AugustoRodrigues, Marcio LourençoStaats, Charley ChristianVainstein, Marilene HenningKmetzsch, Lívia2019-01-11T16:34:03Z2019-01-11T16:34:03Z2018REUWSAAT J. C. V. et al. A predicted mannoprotein participates in Cryptococcus gattii capsular structure. mSphere, v. 3, n. 3, p. 1-17, Mar./Apr. 2018.2379-5042https://www.arca.fiocruz.br/handle/icict/3098510.1128/mSphere.00023-18engAmerican Society for MicrobiologyManoproteínaEstrutura capsularCryptococcus gattiiCapsular polysaccharideMannoproteinA Predicted Mannoprotein Participates in Cryptococcus gattii Capsular Structureinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Laboratório de Fungos de Importância Médica e Biotecnológica. Porto Alegre, RS, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Laboratório de Fungos de Importância Médica e Biotecnológica. Porto Alegre, RS, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Laboratório de Fungos de Importância Médica e Biotecnológica. Porto Alegre, RS, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Laboratório de Fungos de Importância Médica e Biotecnológica. Porto Alegre, RS, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Laboratório de Fungos de Importância Médica e Biotecnológica. Porto Alegre, RS, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Laboratório de Fungos de Importância Médica e Biotecnológica. Porto Alegre, RS, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Goés. Laboratório de Biologia Celular de Leveduras Patogênicas. Rio de Janeiro, RJ, Brazil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Laboratório de Fungos de Importância Médica e Biotecnológica. Porto Alegre, RS, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brazil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Laboratório de Fungos de Importância Médica e Biotecnológica. Porto Alegre, RS, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Laboratório de Fungos de Importância Médica e Biotecnológica. Porto Alegre, RS, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Laboratório de Fungos de Importância Médica e Biotecnológica. Porto Alegre, RS, Brasil.Universidade de Santa Cruz do Sul. Departamento de Biologia e Farmácia. Programa de Pós-Graduação em Promoção da Saúde. Santa Cruz do Sul, RS, Brazil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Departamento de Biologia Molecular e Biotecnologia. Laboratório de Fungos de Importância Médica e Biotecnológica. Porto Alegre, RS, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brazil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Goés. Laboratório de Biologia Celular de Leveduras Patogênicas. Rio de Janeiro, RJ, Brazil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Departamento de Biologia Molecular e Biotecnologia. Laboratório de Fungos de Importância Médica e Biotecnológica. Porto Alegre, RS, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Departamento de Biologia Molecular e Biotecnologia. Laboratório de Fungos de Importância Médica e Biotecnológica. Porto Alegre, RS, Brasil.Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Departamento de Biologia Molecular e Biotecnologia. Laboratório de Fungos de Importância Médica e Biotecnológica. Porto Alegre, RS, Brasil.The yeast-like pathogen Cryptococcus gattii is an etiological agent of cryptococcosis. The major cryptococcal virulence factor is the polysaccharide capsule, which is composed of glucuronoxylomannan (GXM), galactoxylomannan (GalXM), and mannoproteins (MPs). The GXM and GalXM polysaccharides have been extensively characterized; however, there is little information about the role of mannoproteins in capsule assembly and their participation in yeast pathogenicity. The present study characterized the function of a predicted mannoprotein from C. gattii, designated Krp1. Loss-of-function and gain-of-function mutants were generated, and phenotypes associated with the capsular architecture were evaluated. The null mutant cells were more sensitive to a cell wall stressor that disrupts beta-glucan synthesis. Also, these cells displayed increased GXM release to the culture supernatant than the wild-type strain did. The loss of Krp1 influenced cell-associated cryptococcal polysaccharide thickness and phagocytosis by J774.A1 macrophages in the early hours of interaction, but no difference in virulence in a murine model of cryptococcosis was observed. In addition, recombinant Krp1 was antigenic and differentially recognized by serum from an individual with cryptococcosis, but not with serum from an individual with candidiasis. Taken together, these results indicate that C. gattii Krp1 is important for the cell wall structure, thereby influencing capsule assembly, but is not essential for virulence in vivo. Importance: Cryptococcus gattii has the ability to escape from the host's immune system through poorly understood mechanisms and can lead to the death of healthy individuals. The role of mannoproteins in C. gattii pathogenicity is not completely understood. The present work characterized a protein, Kpr1, that is essential for the maintenance of C. gattii main virulence factor, the polysaccharide capsule. Our data contribute to the understanding of the role of Kpr1 in capsule structuring, mainly by modulating the distribution of glucans in C. gattii cell wall.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83101https://www.arca.fiocruz.br/bitstream/icict/30985/1/license.txt425ef259bcb460998407fbfed7c102d0MD51ORIGINALve_Marcio_Lourenço_CDTS_2018.pdfve_Marcio_Lourenço_CDTS_2018.pdfapplication/pdf2818130https://www.arca.fiocruz.br/bitstream/icict/30985/2/ve_Marcio_Louren%c3%a7o_CDTS_2018.pdfebe8857bb055ac12339905fddb49b711MD52TEXTve_Marcio_Lourenço_CDTS_2018.pdf.txtve_Marcio_Lourenço_CDTS_2018.pdf.txtExtracted texttext/plain75170https://www.arca.fiocruz.br/bitstream/icict/30985/3/ve_Marcio_Louren%c3%a7o_CDTS_2018.pdf.txt52687f37db2b32b6e22a28dc6411532dMD53icict/309852019-01-14 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| dc.title.pt_BR.fl_str_mv |
A Predicted Mannoprotein Participates in Cryptococcus gattii Capsular Structure |
| title |
A Predicted Mannoprotein Participates in Cryptococcus gattii Capsular Structure |
| spellingShingle |
A Predicted Mannoprotein Participates in Cryptococcus gattii Capsular Structure Reuwsaat, Julia Catarina Vieira Manoproteína Estrutura capsular Cryptococcus gattii Capsular polysaccharide Mannoprotein |
| title_short |
A Predicted Mannoprotein Participates in Cryptococcus gattii Capsular Structure |
| title_full |
A Predicted Mannoprotein Participates in Cryptococcus gattii Capsular Structure |
| title_fullStr |
A Predicted Mannoprotein Participates in Cryptococcus gattii Capsular Structure |
| title_full_unstemmed |
A Predicted Mannoprotein Participates in Cryptococcus gattii Capsular Structure |
| title_sort |
A Predicted Mannoprotein Participates in Cryptococcus gattii Capsular Structure |
| author |
Reuwsaat, Julia Catarina Vieira |
| author_facet |
Reuwsaat, Julia Catarina Vieira Motta, Heryk Garcia, Ane Wichine Acosta Vasconcelos, Carolina Bettker Marques, Bárbara Machado Oliveira, Natália Kronbauer Rodrigues, Jéssica Ferrareze, Patrícia Aline Gröhns Frases, Susana Lopes, William Barcellos, Vanessa Abreu Squizani, Eamim Daidrê Horta, Jorge André Schrank, Augusto Rodrigues, Marcio Lourenço Staats, Charley Christian Vainstein, Marilene Henning Kmetzsch, Lívia |
| author_role |
author |
| author2 |
Motta, Heryk Garcia, Ane Wichine Acosta Vasconcelos, Carolina Bettker Marques, Bárbara Machado Oliveira, Natália Kronbauer Rodrigues, Jéssica Ferrareze, Patrícia Aline Gröhns Frases, Susana Lopes, William Barcellos, Vanessa Abreu Squizani, Eamim Daidrê Horta, Jorge André Schrank, Augusto Rodrigues, Marcio Lourenço Staats, Charley Christian Vainstein, Marilene Henning Kmetzsch, Lívia |
| author2_role |
author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Reuwsaat, Julia Catarina Vieira Motta, Heryk Garcia, Ane Wichine Acosta Vasconcelos, Carolina Bettker Marques, Bárbara Machado Oliveira, Natália Kronbauer Rodrigues, Jéssica Ferrareze, Patrícia Aline Gröhns Frases, Susana Lopes, William Barcellos, Vanessa Abreu Squizani, Eamim Daidrê Horta, Jorge André Schrank, Augusto Rodrigues, Marcio Lourenço Staats, Charley Christian Vainstein, Marilene Henning Kmetzsch, Lívia |
| dc.subject.other.pt_BR.fl_str_mv |
Manoproteína Estrutura capsular |
| topic |
Manoproteína Estrutura capsular Cryptococcus gattii Capsular polysaccharide Mannoprotein |
| dc.subject.en.pt_BR.fl_str_mv |
Cryptococcus gattii Capsular polysaccharide Mannoprotein |
| description |
Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia. Laboratório de Fungos de Importância Médica e Biotecnológica. Porto Alegre, RS, Brasil. |
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2018 |
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2018 |
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2019-01-11T16:34:03Z |
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2019-01-11T16:34:03Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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REUWSAAT J. C. V. et al. A predicted mannoprotein participates in Cryptococcus gattii capsular structure. mSphere, v. 3, n. 3, p. 1-17, Mar./Apr. 2018. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/30985 |
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2379-5042 |
| dc.identifier.doi.none.fl_str_mv |
10.1128/mSphere.00023-18 |
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REUWSAAT J. C. V. et al. A predicted mannoprotein participates in Cryptococcus gattii capsular structure. mSphere, v. 3, n. 3, p. 1-17, Mar./Apr. 2018. 2379-5042 10.1128/mSphere.00023-18 |
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eng |
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openAccess |
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American Society for Microbiology |
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American Society for Microbiology |
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