Bradykinin enhances Sindbis virus infection in human brain microvascular endothelial cells

Detalhes bibliográficos
Autor(a) principal: Rust, Naiara Miranda
Data de Publicação: 2012
Outros Autores: Papa, Michelle Premazzi, Scovino, Aline Miranda, Silva, Mayara Marques Carneiro da, Silva, Carlos Eduardo Calzavara, Marques Júnior, Ernesto Torres de Azevedo, Peçanha, Ligia Maria Torres, Scharfstein, Julio, Arruda, Luciana Barros de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9045
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Departamento de Virologia. Rio de Janeiro, RJ. Brasil
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spelling Rust, Naiara MirandaPapa, Michelle PremazziScovino, Aline MirandaSilva, Mayara Marques Carneiro daSilva, Carlos Eduardo CalzavaraMarques Júnior, Ernesto Torres de AzevedoPeçanha, Ligia Maria TorresScharfstein, JulioArruda, Luciana Barros de2014-12-04T15:16:21Z2014-12-04T15:16:21Z2012RUST, Naiara Miranda et al. Bradykinin enhances Sindbis virus infection in human brain microvascular endothelial cells. Virology, v. 422, n. 1, p. 81-91, 2012.0042-6822https://www.arca.fiocruz.br/handle/icict/904510.1016/j.virol.2011.10.003.engElsevier Inc.Bradykinin enhances Sindbis virus infection in human brain microvascular endothelial cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Departamento de Virologia. Rio de Janeiro, RJ. BrasilUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Departamento de Virologia. Rio de Janeiro, RJ. BrasilUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ. BrasilFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Departamento de Imunologia Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ. BrasilUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Departamento de Virologia. Rio de Janeiro, RJ. BrasilSindbis virus (SINV) induces inflammatory and vasoactive responses that are associated with rash and arthritis in human infections. The mechanisms underlying infection-associated microvasculopathy are still unknown. We investigated whether endothelial cells infected by SINV are differentially responsive to bradykinin (BK), a potent inducer of inflammatory edema in a broad range of infectious diseases. Human endothelial cells (HBMECs) infected with SINV presented an upregulation of bradykinin B2 receptors (BK2R) expression. Also, BK reduced SINV-induced apoptosis and enhanced virus replication in HBMECs in a way dependent on BK2R, PI3 kinase and ERK signaling. Strikingly, intracerebral infection of mice in the presence of a BK2R antagonist reduced the local viral load. Our data suggest that SINV infection renders human endothelial cells hypersensitive to BK, which increases host cell survival and viral replication. Ongoing studies may clarify if the deregulation of the kinin pathway contributes to infection-associated vasculopathies in life-threatening arbovirus infectionsSindbisBradykininEndothelial cellsApoptosisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9045/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALBradykinin enhances Sindbis virus infection in human brain microvascular endothelial cells.pdfBradykinin enhances Sindbis virus infection in human brain microvascular endothelial cells.pdfapplication/pdf1158448https://www.arca.fiocruz.br/bitstream/icict/9045/2/Bradykinin%20enhances%20Sindbis%20virus%20infection%20in%20human%20brain%20microvascular%20endothelial%20cells.pdf5c208b59273623f263acf5a38adfebf2MD52TEXTBradykinin enhances Sindbis virus infection in human brain microvascular endothelial cells.pdf.txtBradykinin enhances Sindbis virus infection in human brain microvascular endothelial cells.pdf.txtExtracted texttext/plain69329https://www.arca.fiocruz.br/bitstream/icict/9045/3/Bradykinin%20enhances%20Sindbis%20virus%20infection%20in%20human%20brain%20microvascular%20endothelial%20cells.pdf.txtf933766eba72124cb07ff85d27bfe628MD53icict/90452023-07-12 09:30:19.395oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-12T12:30:19Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Bradykinin enhances Sindbis virus infection in human brain microvascular endothelial cells
title Bradykinin enhances Sindbis virus infection in human brain microvascular endothelial cells
spellingShingle Bradykinin enhances Sindbis virus infection in human brain microvascular endothelial cells
Rust, Naiara Miranda
Sindbis
Bradykinin
Endothelial cells
Apoptosis
title_short Bradykinin enhances Sindbis virus infection in human brain microvascular endothelial cells
title_full Bradykinin enhances Sindbis virus infection in human brain microvascular endothelial cells
title_fullStr Bradykinin enhances Sindbis virus infection in human brain microvascular endothelial cells
title_full_unstemmed Bradykinin enhances Sindbis virus infection in human brain microvascular endothelial cells
title_sort Bradykinin enhances Sindbis virus infection in human brain microvascular endothelial cells
author Rust, Naiara Miranda
author_facet Rust, Naiara Miranda
Papa, Michelle Premazzi
Scovino, Aline Miranda
Silva, Mayara Marques Carneiro da
Silva, Carlos Eduardo Calzavara
Marques Júnior, Ernesto Torres de Azevedo
Peçanha, Ligia Maria Torres
Scharfstein, Julio
Arruda, Luciana Barros de
author_role author
author2 Papa, Michelle Premazzi
Scovino, Aline Miranda
Silva, Mayara Marques Carneiro da
Silva, Carlos Eduardo Calzavara
Marques Júnior, Ernesto Torres de Azevedo
Peçanha, Ligia Maria Torres
Scharfstein, Julio
Arruda, Luciana Barros de
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rust, Naiara Miranda
Papa, Michelle Premazzi
Scovino, Aline Miranda
Silva, Mayara Marques Carneiro da
Silva, Carlos Eduardo Calzavara
Marques Júnior, Ernesto Torres de Azevedo
Peçanha, Ligia Maria Torres
Scharfstein, Julio
Arruda, Luciana Barros de
dc.subject.en.pt_BR.fl_str_mv Sindbis
Bradykinin
Endothelial cells
Apoptosis
topic Sindbis
Bradykinin
Endothelial cells
Apoptosis
description Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Departamento de Virologia. Rio de Janeiro, RJ. Brasil
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-12-04T15:16:21Z
dc.date.available.fl_str_mv 2014-12-04T15:16:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RUST, Naiara Miranda et al. Bradykinin enhances Sindbis virus infection in human brain microvascular endothelial cells. Virology, v. 422, n. 1, p. 81-91, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9045
dc.identifier.issn.none.fl_str_mv 0042-6822
dc.identifier.doi.none.fl_str_mv 10.1016/j.virol.2011.10.003.
identifier_str_mv RUST, Naiara Miranda et al. Bradykinin enhances Sindbis virus infection in human brain microvascular endothelial cells. Virology, v. 422, n. 1, p. 81-91, 2012.
0042-6822
10.1016/j.virol.2011.10.003.
url https://www.arca.fiocruz.br/handle/icict/9045
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier Inc.
publisher.none.fl_str_mv Elsevier Inc.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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