Binding of excreted and/or secreted products of adult hookworms to human NK cells in Necator americanus-infected individuals from Brazil

Detalhes bibliográficos
Autor(a) principal: Carvalho, Andréa Teixeira de
Data de Publicação: 2008
Outros Autores: Fujiwara, Ricardo Toshio, Stemmy, Erik J., Olive, Denise, Damsker, Jesse M., Loukas, Alex, Oliveira, Rodrigo Corrêa, Constant, Stephanie L., Bethony, Jeffrey Michael
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/54365
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil/Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Análises Clínicas. Ouro Preto, MG, Brasil
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spelling Carvalho, Andréa Teixeira deFujiwara, Ricardo ToshioStemmy, Erik J.Olive, DeniseDamsker, Jesse M.Loukas, AlexOliveira, Rodrigo CorrêaConstant, Stephanie L.Bethony, Jeffrey Michael2022-08-03T17:51:56Z2022-08-03T17:51:56Z2008CARVALHO, Andréa Teixeira de et al. Binding of excreted and/or secreted products of adult hookworms to human NK cells in Necator americanus-infected individuals from Brazil. Infect Immun., v. 76, n. 12, p. 5810-5816, 2008. doi: 10.1128/IAI.00419-080019-9567https://www.arca.fiocruz.br/handle/icict/5436510.1128/IAI.00419-08engAmerican Society For MicrobiologyBinding of excreted and/or secreted products of adult hookworms to human NK cells in Necator americanus-infected individuals from Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil/Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Análises Clínicas. Ouro Preto, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil/Department of Microbiology. Immunology and Tropical Medicine. The George Washington University Medical Center. Washington, DC, USADepartment of Microbiology. Immunology and Tropical Medicine. The George Washington University Medical Center. Washington, DC, USADepartment of Microbiology. Immunology and Tropical Medicine. The George Washington University Medical Center. Washington, DC, USADepartment of Microbiology. Immunology and Tropical Medicine. The George Washington University Medical Center. Washington, DC, USAQueensland Institute of Medical Research. Brisbane, Queensland, AustraliaFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, BrasilDepartment of Microbiology. Immunology and Tropical Medicine. The George Washington University Medical Center. Washington, DC, USAFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil/Department of Microbiology. Immunology and Tropical Medicine. The George Washington University Medical Center. Washington, DC, USAThe impact of the interaction between excreted and/or secreted (ES) Necator americanus products and NK cells from Necator-infected individuals was analyzed. We investigated the binding of ES products to NK cells, the expression of NK cell receptors (CD56, CD159a/NKG2A, CD314/NKG2D, CD335/NKp46, and KLRF1/NKp80), the frequency of gamma interferon (IFN-gamma)-producing NK cells after whole-blood in vitro stimulation, and the capacity of N. americanus ES products to induce NK cell chemotaxis. In contrast to those from noninfected individuals, NK cells from Necator-infected individuals demonstrated no binding with N. americanus ES products. This phenomenon was not due to alterations in NK cell receptor expression in infected subjects and could not be reproduced with NK cells from uninfected individuals by incubation with immunoregulatory cytokines (interleukin-10/transforming growth factor beta). Further, we found that a significantly greater percentage of NK cells from infected subjects than NK cells from uninfected individuals spontaneously produced IFN-gamma upon ex vivo culture. Our findings support a model whereby NK cells from Necator-infected individuals may interact with ES products, making these cells refractory to binding with exogenous ES products. During N. americanus infection, human NK cells are attracted to the site of infection by chemotactic ES products produced by adult Necator worms in the gut mucosa. Binding of ES products causes the NK cells to become activated and secrete IFN-gamma locally, thereby contributing to the adult hookworm's ability to evade host immune responses.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/54365/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALBinding of excreted.pdfBinding of excreted.pdfapplication/pdf497780https://www.arca.fiocruz.br/bitstream/icict/54365/2/Binding%20of%20excreted.pdfd6b0f4fd7c65d5a0714fde6b0fcbf0b4MD52icict/543652022-08-03 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dc.title.pt_BR.fl_str_mv Binding of excreted and/or secreted products of adult hookworms to human NK cells in Necator americanus-infected individuals from Brazil
title Binding of excreted and/or secreted products of adult hookworms to human NK cells in Necator americanus-infected individuals from Brazil
spellingShingle Binding of excreted and/or secreted products of adult hookworms to human NK cells in Necator americanus-infected individuals from Brazil
Carvalho, Andréa Teixeira de
title_short Binding of excreted and/or secreted products of adult hookworms to human NK cells in Necator americanus-infected individuals from Brazil
title_full Binding of excreted and/or secreted products of adult hookworms to human NK cells in Necator americanus-infected individuals from Brazil
title_fullStr Binding of excreted and/or secreted products of adult hookworms to human NK cells in Necator americanus-infected individuals from Brazil
title_full_unstemmed Binding of excreted and/or secreted products of adult hookworms to human NK cells in Necator americanus-infected individuals from Brazil
title_sort Binding of excreted and/or secreted products of adult hookworms to human NK cells in Necator americanus-infected individuals from Brazil
author Carvalho, Andréa Teixeira de
author_facet Carvalho, Andréa Teixeira de
Fujiwara, Ricardo Toshio
Stemmy, Erik J.
Olive, Denise
Damsker, Jesse M.
Loukas, Alex
Oliveira, Rodrigo Corrêa
Constant, Stephanie L.
Bethony, Jeffrey Michael
author_role author
author2 Fujiwara, Ricardo Toshio
Stemmy, Erik J.
Olive, Denise
Damsker, Jesse M.
Loukas, Alex
Oliveira, Rodrigo Corrêa
Constant, Stephanie L.
Bethony, Jeffrey Michael
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Andréa Teixeira de
Fujiwara, Ricardo Toshio
Stemmy, Erik J.
Olive, Denise
Damsker, Jesse M.
Loukas, Alex
Oliveira, Rodrigo Corrêa
Constant, Stephanie L.
Bethony, Jeffrey Michael
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil/Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Análises Clínicas. Ouro Preto, MG, Brasil
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2022-08-03T17:51:56Z
dc.date.available.fl_str_mv 2022-08-03T17:51:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, Andréa Teixeira de et al. Binding of excreted and/or secreted products of adult hookworms to human NK cells in Necator americanus-infected individuals from Brazil. Infect Immun., v. 76, n. 12, p. 5810-5816, 2008. doi: 10.1128/IAI.00419-08
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/54365
dc.identifier.issn.pt_BR.fl_str_mv 0019-9567
dc.identifier.doi.none.fl_str_mv 10.1128/IAI.00419-08
identifier_str_mv CARVALHO, Andréa Teixeira de et al. Binding of excreted and/or secreted products of adult hookworms to human NK cells in Necator americanus-infected individuals from Brazil. Infect Immun., v. 76, n. 12, p. 5810-5816, 2008. doi: 10.1128/IAI.00419-08
0019-9567
10.1128/IAI.00419-08
url https://www.arca.fiocruz.br/handle/icict/54365
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society For Microbiology
publisher.none.fl_str_mv American Society For Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/54365/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/54365/2/Binding%20of%20excreted.pdf
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