Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis
Autor(a) principal: | |
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Data de Publicação: | 1992 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/43489 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunidade Celular e Humoral em Protozooses, Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil. |
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Nunes, Marise P.Sobral, Ana Cristina L.Coutinho, Sérgio G.2020-09-20T20:46:47Z2020-09-20T20:46:47Z1992NUNES, Marise P.; SOBRAL, Ana Cristina L.; COUTINHO, Sérgio G. Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 87, n. 4, p. 503-509, Oct./Dec.1992.0074-0276https://www.arca.fiocruz.br/handle/icict/4348910.1590/S0074-027619920004000071678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Trypanosoma cruziInfecção experimentalLimitando a análise de diluiçãoTrypanosoma cruziLimiting dilution analysisExperimental infectionQuantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunidade Celular e Humoral em Protozooses, Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunidade Celular e Humoral em Protozooses, Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunidade Celular e Humoral em Protozooses, Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.Limiting dilution analysis was used to quantify Trypanosoma cruzi in the lymph nodes, liver and heart of Swiss and C57 B1/10 mice. The results showed that, in Swiss and B1/10 mice infected with T. cruzi Y strain, the number of parasites/mg of tissue increased during the course of the infection in both types of mice, although a grater number of parasites were observed in heart tissue from Swiss mice than from B1/10. With regard to liver tissue, it was observed that the parasite load in the initial phase of infection was higher than in heart. In experiments using T. cruzi Colombian strain, the parasite load in the heart of Swiss and B1/10 mice increased relatively slowly, although high levels of parasitization were nonetheless observable by the end of the infection. As for the liver and lymph nodes, the concentration of parasites was lower over the entire course of infection than in heart. Both strains thus maintained their characteristic tissue tropisms. The limiting dilution assay (LDA) proved to be an appropriate method for more precise quantification of T. cruzi, comparing favorably with other direct microscopic methods that only give approximate scores.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43489/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMariseP_Nunes_etal_IOC_1992.pdfMariseP_Nunes_etal_IOC_1992.pdfapplication/pdf314072https://www.arca.fiocruz.br/bitstream/icict/43489/2/MariseP_Nunes_etal_IOC_1992.pdf6316e4679c503f1d020f10dad1d95391MD52TEXTMariseP_Nunes_etal_IOC_1992.pdf.txtMariseP_Nunes_etal_IOC_1992.pdf.txtExtracted texttext/plain7https://www.arca.fiocruz.br/bitstream/icict/43489/3/MariseP_Nunes_etal_IOC_1992.pdf.txt212b0306580d4f0044d18f9a3edcc832MD53icict/434892020-09-21 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dc.title.pt_BR.fl_str_mv |
Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis |
title |
Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis |
spellingShingle |
Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis Nunes, Marise P. Trypanosoma cruzi Infecção experimental Limitando a análise de diluição Trypanosoma cruzi Limiting dilution analysis Experimental infection |
title_short |
Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis |
title_full |
Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis |
title_fullStr |
Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis |
title_full_unstemmed |
Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis |
title_sort |
Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis |
author |
Nunes, Marise P. |
author_facet |
Nunes, Marise P. Sobral, Ana Cristina L. Coutinho, Sérgio G. |
author_role |
author |
author2 |
Sobral, Ana Cristina L. Coutinho, Sérgio G. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Nunes, Marise P. Sobral, Ana Cristina L. Coutinho, Sérgio G. |
dc.subject.other.pt_BR.fl_str_mv |
Trypanosoma cruzi Infecção experimental Limitando a análise de diluição |
topic |
Trypanosoma cruzi Infecção experimental Limitando a análise de diluição Trypanosoma cruzi Limiting dilution analysis Experimental infection |
dc.subject.en.pt_BR.fl_str_mv |
Trypanosoma cruzi Limiting dilution analysis Experimental infection |
description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunidade Celular e Humoral em Protozooses, Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil. |
publishDate |
1992 |
dc.date.issued.fl_str_mv |
1992 |
dc.date.accessioned.fl_str_mv |
2020-09-20T20:46:47Z |
dc.date.available.fl_str_mv |
2020-09-20T20:46:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
NUNES, Marise P.; SOBRAL, Ana Cristina L.; COUTINHO, Sérgio G. Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 87, n. 4, p. 503-509, Oct./Dec.1992. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/43489 |
dc.identifier.issn.pt_BR.fl_str_mv |
0074-0276 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0074-02761992000400007 |
dc.identifier.eissn.none.fl_str_mv |
1678-8060 |
identifier_str_mv |
NUNES, Marise P.; SOBRAL, Ana Cristina L.; COUTINHO, Sérgio G. Quantification of Trypanosoma cruzi in the heart, lymph nodes and liver of experimentally inffected mice, using limiting dilution analysis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 87, n. 4, p. 503-509, Oct./Dec.1992. 0074-0276 10.1590/S0074-02761992000400007 1678-8060 |
url |
https://www.arca.fiocruz.br/handle/icict/43489 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
publisher.none.fl_str_mv |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Fundação Oswaldo Cruz (FIOCRUZ) |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
collection |
Repositório Institucional da FIOCRUZ (ARCA) |
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Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
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repositorio.arca@fiocruz.br |
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