Real-Time PCR Reveals Rapid Dissemination of Leptospira interrogans after Intraperitoneal and Conjunctival Inoculation of Hamsters
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1128/IAI.00094-16 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/17886 |
Resumo: | National Institutes of Health and the National Council of Scientific and Technological Development (CNPq), Brazilian Ministry of Science and Technology. |
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Wunder Junior, Elsio AugustoFigueira, Claudio PereiraSantos, Gisele RLourdault, KristelMatthias, Michael AVinetz, Joseph MRamos, Eduardo Antônio GonçalvesHaake, David APicardeau, MathieuReis, Mitermayer Galvão dosKo, Albert Icksang2017-02-23T11:56:14Z2017-02-23T11:56:14Z2016WUNDER JÚNIOR, E. A. et al. Real-Time PCR Reveals Rapid Dissemination of Leptospira interrogans after Intraperitoneal and Conjunctival Inoculation of Hamsters. Infection and Immunity, v. 84, n. 7, p. 2105-2115, 2016.0019-9567https://www.arca.fiocruz.br/handle/icict/1788610.1128/IAI.00094-16National Institutes of Health and the National Council of Scientific and Technological Development (CNPq), Brazilian Ministry of Science and Technology.Yale School of Public Health. Epidemiology of Microbial Disease Division. New Haven, Connecticut, USA / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilInstitut Pasteur. Unité de Biologie des Spirochètes. Paris, France / University of California. David Geffen School of Medicine. Department of Medicine. Los Angeles, Los Angeles, California, USAUniversity of California San Diego School of Medicine. Division of Infectious Diseases. Department of Medicine. La Jolla, California, USAUniversity of California San Diego School of Medicine. Division of Infectious Diseases. Department of Medicine. La Jolla, California, USAFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversity of California. David Geffen School of Medicine. Department of Medicine. Los Angeles, Los Angeles, California, USAInstitut Pasteur. Unité de Biologie des Spirochètes. Paris, FranceFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilYale School of Public Health. Epidemiology of Microbial Disease Division. New Haven, Connecticut, USA / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilThe pathogen Leptospira interrogans is a highly motile spirochete that causes acute and fulminant infections in humans and other accidental hosts. Hematogenous dissemination is important for infection by the pathogen but remains poorly understood because few animal model studies have used sensitive tools to quantify the bacteria. We evaluated the kinetics of leptospiral infection in Golden Syrian hamsters by a sensitive quantitative real-time PCR (TaqMan) with lipl32 as the target gene. The dissemination and bacterial burden were measured after intraperitoneal infection with a high dose (10(8)) or low dose (2.5 × 10(2)) of leptospires. We also examined the conjunctival challenge route to mimic the natural history of infection. Quantification of leptospires in perfused animals revealed that pathogens were detected in all organs of intraperitoneally infected hamsters, including the eye and brain, within 1 h after inoculation of 10(8) virulent L. interrogans bacteria. Peaks of 10(5) to 10(8) leptospires per gram or per milliliter were achieved in blood and all tissues between day 4 and day 8 after intraperitoneal inoculation of high- and low-dose challenges, respectively, coinciding with macroscopic and histological changes. The conjunctival route resulted in a delay in the time to peak organ burden in comparison to intraperitoneal infection, indicating that although infection could be established, penetration efficiency was low across this epithelial barrier. Surprisingly, infection with a large inoculum of high-passage-number attenuated L. interrogans strains resulted in dissemination to all organs in the first 4 days postinfection, albeit with a lower burden, followed by clearance from the blood and organs 7 days postinfection and survival of all animals. These results demonstrate that leptospiral dissemination and tissue invasion occur. In contrast, development of a critical level of tissue burden and pathology are dependent on the virulence of the infecting strain.engAmerican Society for MicrobiologyLeptospira interrogansLeptospiroseInfecçãoHamstersAnimaisLeptospira interrogansLeptospirosisInfectionHamstersAnimalsReal-Time PCR Reveals Rapid Dissemination of Leptospira interrogans after Intraperitoneal and Conjunctival Inoculation of Hamstersinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/17886/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALWunder Jr EA Real-time PCR....pdfWunder Jr EA Real-time 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| dc.title.pt_BR.fl_str_mv |
Real-Time PCR Reveals Rapid Dissemination of Leptospira interrogans after Intraperitoneal and Conjunctival Inoculation of Hamsters |
| title |
Real-Time PCR Reveals Rapid Dissemination of Leptospira interrogans after Intraperitoneal and Conjunctival Inoculation of Hamsters |
| spellingShingle |
Real-Time PCR Reveals Rapid Dissemination of Leptospira interrogans after Intraperitoneal and Conjunctival Inoculation of Hamsters Wunder Junior, Elsio Augusto Leptospira interrogans Leptospirose Infecção Hamsters Animais Leptospira interrogans Leptospirosis Infection Hamsters Animals |
| title_short |
Real-Time PCR Reveals Rapid Dissemination of Leptospira interrogans after Intraperitoneal and Conjunctival Inoculation of Hamsters |
| title_full |
Real-Time PCR Reveals Rapid Dissemination of Leptospira interrogans after Intraperitoneal and Conjunctival Inoculation of Hamsters |
| title_fullStr |
Real-Time PCR Reveals Rapid Dissemination of Leptospira interrogans after Intraperitoneal and Conjunctival Inoculation of Hamsters |
| title_full_unstemmed |
Real-Time PCR Reveals Rapid Dissemination of Leptospira interrogans after Intraperitoneal and Conjunctival Inoculation of Hamsters |
| title_sort |
Real-Time PCR Reveals Rapid Dissemination of Leptospira interrogans after Intraperitoneal and Conjunctival Inoculation of Hamsters |
| author |
Wunder Junior, Elsio Augusto |
| author_facet |
Wunder Junior, Elsio Augusto Figueira, Claudio Pereira Santos, Gisele R Lourdault, Kristel Matthias, Michael A Vinetz, Joseph M Ramos, Eduardo Antônio Gonçalves Haake, David A Picardeau, Mathieu Reis, Mitermayer Galvão dos Ko, Albert Icksang |
| author_role |
author |
| author2 |
Figueira, Claudio Pereira Santos, Gisele R Lourdault, Kristel Matthias, Michael A Vinetz, Joseph M Ramos, Eduardo Antônio Gonçalves Haake, David A Picardeau, Mathieu Reis, Mitermayer Galvão dos Ko, Albert Icksang |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Wunder Junior, Elsio Augusto Figueira, Claudio Pereira Santos, Gisele R Lourdault, Kristel Matthias, Michael A Vinetz, Joseph M Ramos, Eduardo Antônio Gonçalves Haake, David A Picardeau, Mathieu Reis, Mitermayer Galvão dos Ko, Albert Icksang |
| dc.subject.other.pt_BR.fl_str_mv |
Leptospira interrogans Leptospirose Infecção Hamsters Animais |
| topic |
Leptospira interrogans Leptospirose Infecção Hamsters Animais Leptospira interrogans Leptospirosis Infection Hamsters Animals |
| dc.subject.en.pt_BR.fl_str_mv |
Leptospira interrogans Leptospirosis Infection Hamsters Animals |
| description |
National Institutes of Health and the National Council of Scientific and Technological Development (CNPq), Brazilian Ministry of Science and Technology. |
| publishDate |
2016 |
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2016 |
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2017-02-23T11:56:14Z |
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2017-02-23T11:56:14Z |
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WUNDER JÚNIOR, E. A. et al. Real-Time PCR Reveals Rapid Dissemination of Leptospira interrogans after Intraperitoneal and Conjunctival Inoculation of Hamsters. Infection and Immunity, v. 84, n. 7, p. 2105-2115, 2016. |
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https://www.arca.fiocruz.br/handle/icict/17886 |
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0019-9567 |
| dc.identifier.doi.none.fl_str_mv |
10.1128/IAI.00094-16 |
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WUNDER JÚNIOR, E. A. et al. Real-Time PCR Reveals Rapid Dissemination of Leptospira interrogans after Intraperitoneal and Conjunctival Inoculation of Hamsters. Infection and Immunity, v. 84, n. 7, p. 2105-2115, 2016. 0019-9567 10.1128/IAI.00094-16 |
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American Society for Microbiology |
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American Society for Microbiology |
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