Investigação sobre a presença de leptospiras nos ovários de hamsters experimentalmente infectados com Leptospiras interrogans sorotipo pomona

Detalhes bibliográficos
Autor(a) principal: Camargo, Claudio Roberto de Almeida
Data de Publicação: 1993
Outros Autores: Vasconcellos, Silvio Arruda, Nürmberger Júnior, Rodolfo, Passos, Estevão de Camargo, Morais, Zenaíde Maria de, Visintin, José Antonio
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/52024
Resumo: After inoculating L. interrogans serovar pomona in 75 primiparous hamsters (Mesocricetus auratus), the invasiveness of leptospires into lhe ovaries and lhe ability in causing ovary morphologic alterations were investigated by means of microscopic examination and bacterial isolation. For this purpose, 75 hamsters were inoculated with 0.5 ml of virulent strain containing 30-40 leptospires by the microscopic field and the other 15 hamsters were held as the uninfected controls. Signs and symptoms (prostration, tachypnea, rufled hair, jaundice, and nasal, bucal and perineal hemorrage) were detected in all inoculated animals. The animals were killed in the agonic state of the illness, which were done through 4th and 7th day post inoculation. The ovaries were taken asseptically during the necropsies, thoroughly washed using the sterile phosphate buffered saline, in order to eliminate the possible external contamination. The fresh ovary samples were submitted to the dark field direct microscopic examination. After the formalin fixation, the specimens were stained by means of histopathologic techniques using the Levaditi and Hematoxylin Eosin stains. The ovary smears were also examined by the direct fluorescent antibody technique andlhe bacterial isolation was carried out in the Fletcher’s medium. The dark field direct microscopic examination was found tobe less sensitive in demonstrating the presence of leptospiresin the ovaries. In those specimens stained by the Lcvadititechnique, leptospires were visualized in different ovaryinternal structures, involving the interspace, pellucid zone andin the inner ovules. Through the histopathologic examination,typical morphologic alterations resembling acute infiamatoryprocess were found in 57% of ovaries examined.
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spelling Investigação sobre a presença de leptospiras nos ovários de hamsters experimentalmente infectados com Leptospiras interrogans sorotipo pomonaInvestigation on the presence of leptospires in ovaries of hamsters experimentally infected whith Leptospiras interrogans serovar pomonaLeptospirosisLeptospira interrogans (isolation)Ovaries (hamsters)LeptospiroseLeptospira interrogans (isolamento)Ovários (hamster)After inoculating L. interrogans serovar pomona in 75 primiparous hamsters (Mesocricetus auratus), the invasiveness of leptospires into lhe ovaries and lhe ability in causing ovary morphologic alterations were investigated by means of microscopic examination and bacterial isolation. For this purpose, 75 hamsters were inoculated with 0.5 ml of virulent strain containing 30-40 leptospires by the microscopic field and the other 15 hamsters were held as the uninfected controls. Signs and symptoms (prostration, tachypnea, rufled hair, jaundice, and nasal, bucal and perineal hemorrage) were detected in all inoculated animals. The animals were killed in the agonic state of the illness, which were done through 4th and 7th day post inoculation. The ovaries were taken asseptically during the necropsies, thoroughly washed using the sterile phosphate buffered saline, in order to eliminate the possible external contamination. The fresh ovary samples were submitted to the dark field direct microscopic examination. After the formalin fixation, the specimens were stained by means of histopathologic techniques using the Levaditi and Hematoxylin Eosin stains. The ovary smears were also examined by the direct fluorescent antibody technique andlhe bacterial isolation was carried out in the Fletcher’s medium. The dark field direct microscopic examination was found tobe less sensitive in demonstrating the presence of leptospiresin the ovaries. In those specimens stained by the Lcvadititechnique, leptospires were visualized in different ovaryinternal structures, involving the interspace, pellucid zone andin the inner ovules. Through the histopathologic examination,typical morphologic alterations resembling acute infiamatoryprocess were found in 57% of ovaries examined.De 90 hamsters primíparas com 80 a 120 gramas de peso vivo, 75 foram inoculadas com a dose individual de 0,5 ml de estirpe virulenta do sorotipo pomona (30 a 40 leptospiras ativas por campo microscópico, no aumento de 200 vezes) e as 15 remanescentes constituíram o grupo testemunho não infectado. Todos os animais tratados com leptospiras apresentaram os sinais da infecção (prostração, taquipnéia, eriçamento do pelame, icterícia e hemorragias nasal, bucal e perineal) e foram sacrificados por ocasião da fase agônica da doença, situada entre o quarto e o sétimo dia da inoculação. Nesta oportunidade, os ovários foram colhidos em condições assépticas e submetidos à técnica de visualização de leptospiras (exame direto em microscopia de campo escuro, coloração argêntica de Levaditi e reação de imunofluorescência direta), cultivo em meio de Fletcher e exame histopatológico (coloração de hematoxilina e eosina). A ocorrência de uma possível contaminação estabelecida durante a retirada dos ovários foi investigada através da lavagem em solução salina tamponada estéril. As leptospiras foram demonstradas em todos os ovários do grupo de animais experimentalmente inoculados (lavados e não lavados), através da coloração de Levaditi, reação imunofluorescência direta e também no cultivo em meio de Fletcher. O exame direto em microscopia de campo escuro mostrou ser uma técnica muito pouco sensível. O exame das preparações submetidas à coloração argêntica possibilitou a visualização de leptospiras em diferentes estruturas dos ovários, incluindo: o interstício, a zona pelúcida e o interior dos óvulos. Os exames histopatológicos permitiram observar as alterações morfológicas típicas de um processo inflamatório agudo em 57% dos ovários examinados.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia1993-12-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/5202410.11606/issn.1678-4456.bjvras.1993.52024Brazilian Journal of Veterinary Research and Animal Science; Vol. 30 Núm. 2 (1993); 129-135Brazilian Journal of Veterinary Research and Animal Science; Vol. 30 No. 2 (1993); 129-135Brazilian Journal of Veterinary Research and Animal Science; v. 30 n. 2 (1993); 129-135Brazilian Journal of Veterinary Research and Animal Science; V. 30 N. 2 (1993); 129-1351678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/52024/56074Camargo, Claudio Roberto de AlmeidaVasconcellos, Silvio ArrudaNürmberger Júnior, RodolfoPassos, Estevão de CamargoMorais, Zenaíde Maria deVisintin, José Antonioinfo:eu-repo/semantics/openAccess2020-06-23T04:54:00Zoai:revistas.usp.br:article/52024Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:26.726569Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Investigação sobre a presença de leptospiras nos ovários de hamsters experimentalmente infectados com Leptospiras interrogans sorotipo pomona
Investigation on the presence of leptospires in ovaries of hamsters experimentally infected whith Leptospiras interrogans serovar pomona
title Investigação sobre a presença de leptospiras nos ovários de hamsters experimentalmente infectados com Leptospiras interrogans sorotipo pomona
spellingShingle Investigação sobre a presença de leptospiras nos ovários de hamsters experimentalmente infectados com Leptospiras interrogans sorotipo pomona
Camargo, Claudio Roberto de Almeida
Leptospirosis
Leptospira interrogans (isolation)
Ovaries (hamsters)
Leptospirose
Leptospira interrogans (isolamento)
Ovários (hamster)
title_short Investigação sobre a presença de leptospiras nos ovários de hamsters experimentalmente infectados com Leptospiras interrogans sorotipo pomona
title_full Investigação sobre a presença de leptospiras nos ovários de hamsters experimentalmente infectados com Leptospiras interrogans sorotipo pomona
title_fullStr Investigação sobre a presença de leptospiras nos ovários de hamsters experimentalmente infectados com Leptospiras interrogans sorotipo pomona
title_full_unstemmed Investigação sobre a presença de leptospiras nos ovários de hamsters experimentalmente infectados com Leptospiras interrogans sorotipo pomona
title_sort Investigação sobre a presença de leptospiras nos ovários de hamsters experimentalmente infectados com Leptospiras interrogans sorotipo pomona
author Camargo, Claudio Roberto de Almeida
author_facet Camargo, Claudio Roberto de Almeida
Vasconcellos, Silvio Arruda
Nürmberger Júnior, Rodolfo
Passos, Estevão de Camargo
Morais, Zenaíde Maria de
Visintin, José Antonio
author_role author
author2 Vasconcellos, Silvio Arruda
Nürmberger Júnior, Rodolfo
Passos, Estevão de Camargo
Morais, Zenaíde Maria de
Visintin, José Antonio
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Camargo, Claudio Roberto de Almeida
Vasconcellos, Silvio Arruda
Nürmberger Júnior, Rodolfo
Passos, Estevão de Camargo
Morais, Zenaíde Maria de
Visintin, José Antonio
dc.subject.por.fl_str_mv Leptospirosis
Leptospira interrogans (isolation)
Ovaries (hamsters)
Leptospirose
Leptospira interrogans (isolamento)
Ovários (hamster)
topic Leptospirosis
Leptospira interrogans (isolation)
Ovaries (hamsters)
Leptospirose
Leptospira interrogans (isolamento)
Ovários (hamster)
description After inoculating L. interrogans serovar pomona in 75 primiparous hamsters (Mesocricetus auratus), the invasiveness of leptospires into lhe ovaries and lhe ability in causing ovary morphologic alterations were investigated by means of microscopic examination and bacterial isolation. For this purpose, 75 hamsters were inoculated with 0.5 ml of virulent strain containing 30-40 leptospires by the microscopic field and the other 15 hamsters were held as the uninfected controls. Signs and symptoms (prostration, tachypnea, rufled hair, jaundice, and nasal, bucal and perineal hemorrage) were detected in all inoculated animals. The animals were killed in the agonic state of the illness, which were done through 4th and 7th day post inoculation. The ovaries were taken asseptically during the necropsies, thoroughly washed using the sterile phosphate buffered saline, in order to eliminate the possible external contamination. The fresh ovary samples were submitted to the dark field direct microscopic examination. After the formalin fixation, the specimens were stained by means of histopathologic techniques using the Levaditi and Hematoxylin Eosin stains. The ovary smears were also examined by the direct fluorescent antibody technique andlhe bacterial isolation was carried out in the Fletcher’s medium. The dark field direct microscopic examination was found tobe less sensitive in demonstrating the presence of leptospiresin the ovaries. In those specimens stained by the Lcvadititechnique, leptospires were visualized in different ovaryinternal structures, involving the interspace, pellucid zone andin the inner ovules. Through the histopathologic examination,typical morphologic alterations resembling acute infiamatoryprocess were found in 57% of ovaries examined.
publishDate 1993
dc.date.none.fl_str_mv 1993-12-02
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/52024
10.11606/issn.1678-4456.bjvras.1993.52024
url https://www.revistas.usp.br/bjvras/article/view/52024
identifier_str_mv 10.11606/issn.1678-4456.bjvras.1993.52024
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/52024/56074
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 30 Núm. 2 (1993); 129-135
Brazilian Journal of Veterinary Research and Animal Science; Vol. 30 No. 2 (1993); 129-135
Brazilian Journal of Veterinary Research and Animal Science; v. 30 n. 2 (1993); 129-135
Brazilian Journal of Veterinary Research and Animal Science; V. 30 N. 2 (1993); 129-135
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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