In vitro differentiation of human dendritic cells and their markers in leishmania infection

Detalhes bibliográficos
Autor(a) principal: Paixão, Amanda R
Data de Publicação: 2022
Outros Autores: Tibúrcio, Rafael, Carvalho, Elaine, Silva, Thaílla, Veras, Patrícia S. T, Tavares, Natalia M, Menezes, Juliana P. B. de, Brodskyn, Cláudia I
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/54914
Resumo: Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Hospedeiro-Parasita e Epidemiologia. Salvador, BA, Brasil.
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spelling Paixão, Amanda RTibúrcio, RafaelCarvalho, ElaineSilva, ThaíllaVeras, Patrícia S. TTavares, Natalia MMenezes, Juliana P. B. deBrodskyn, Cláudia I2022-09-28T15:15:46Z2022-09-28T15:15:46Z2022PAIXÃO, Amanda R. et al. In vitro differentiation of human dendritic cells and their markers in leishmania infection. Journal of Visualized Experiments, v. 182, p. 1-14, 2022.1940-087Xhttps://www.arca.fiocruz.br/handle/icict/5491410.3791/62794engMyJove CorporationDendritic CellsInfectionsLeishmaniaCélulas dendríticasInfecçãoLeishmaniaDendritic CellsInfectionIn vitro differentiation of human dendritic cells and their markers in leishmania infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Hospedeiro-Parasita e Epidemiologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Hospedeiro-Parasita e Epidemiologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Hospedeiro-Parasita e Epidemiologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Hospedeiro-Parasita e Epidemiologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Hospedeiro-Parasita e Epidemiologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Hospedeiro-Parasita e Epidemiologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Hospedeiro-Parasita e Epidemiologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Hospedeiro-Parasita e Epidemiologia. Salvador, BA, Brasil.Leishmaniasis comprises a collection of clinical manifestations associated with the infection of obligate intracellular protozoans, Leishmania. The life cycle of Leishmania parasites consists of two alternating life stages (amastigotes and promastigotes), during which parasites reside within either arthropod vectors or vertebrate hosts, respectively. Notably, the complex interactions between Leishmania parasites and several cells of the immune system largely influence the outcome of infection. Importantly, although macrophages are known to be the main host niche for Leishmania replication, parasites are also phagocytosed by other innate immune cells, such as neutrophils and dendritic cells (DCs). DCs play a major role in bridging the innate and adaptive branches of immunity and thus orchestrate immune responses against a wide range of pathogens. The mechanisms by which Leishmania and DCs interact remain unclear and involve aspects of pathogen capture, the dynamics of DC maturation and activation, DC migration to draining lymph node (dLNs), and antigen presentation to T cells. Although a large body of studies support the notion that DCs play a dual role in modulating immune responses against Leishmania, the participation of these cells in susceptibility or resistance to Leishmania remains poorly understood. After infection, DCs undergo a maturation process associated with the upregulation of surface major histocompatibility complex (MHC) II, in addition to costimulatory molecules (namely, CD40, CD80, and CD86). Understanding the role of DCs in infection outcome is crucial to developing therapeutic and prophylactic strategies to modulate the immune response against Leishmania. This paper describes a method for the characterization of Leishmania-DC interaction. This detailed protocol provides guidance throughout the steps of DC differentiation, the characterization of cell surface molecules, and infection protocols, allowing scientists to investigate DC response to Leishmania infection and gain insight into the roles played by these cells in the course of infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/54914/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPaixão, Amanda - in vitro differentiation human.pdfPaixão, Amanda - in vitro differentiation human.pdfapplication/pdf579572https://www.arca.fiocruz.br/bitstream/icict/54914/2/Paix%c3%a3o%2c%20Amanda%20-%20in%20vitro%20differentiation%20human.pdf73db17f995be2c55394d94afbb791958MD52icict/549142023-03-15 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dc.title.en_US.fl_str_mv In vitro differentiation of human dendritic cells and their markers in leishmania infection
title In vitro differentiation of human dendritic cells and their markers in leishmania infection
spellingShingle In vitro differentiation of human dendritic cells and their markers in leishmania infection
Paixão, Amanda R
Dendritic Cells
Infections
Leishmania
Células dendríticas
Infecção
Leishmania
Dendritic Cells
Infection
title_short In vitro differentiation of human dendritic cells and their markers in leishmania infection
title_full In vitro differentiation of human dendritic cells and their markers in leishmania infection
title_fullStr In vitro differentiation of human dendritic cells and their markers in leishmania infection
title_full_unstemmed In vitro differentiation of human dendritic cells and their markers in leishmania infection
title_sort In vitro differentiation of human dendritic cells and their markers in leishmania infection
author Paixão, Amanda R
author_facet Paixão, Amanda R
Tibúrcio, Rafael
Carvalho, Elaine
Silva, Thaílla
Veras, Patrícia S. T
Tavares, Natalia M
Menezes, Juliana P. B. de
Brodskyn, Cláudia I
author_role author
author2 Tibúrcio, Rafael
Carvalho, Elaine
Silva, Thaílla
Veras, Patrícia S. T
Tavares, Natalia M
Menezes, Juliana P. B. de
Brodskyn, Cláudia I
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Paixão, Amanda R
Tibúrcio, Rafael
Carvalho, Elaine
Silva, Thaílla
Veras, Patrícia S. T
Tavares, Natalia M
Menezes, Juliana P. B. de
Brodskyn, Cláudia I
dc.subject.mesh.en_US.fl_str_mv Dendritic Cells
Infections
topic Dendritic Cells
Infections
Leishmania
Células dendríticas
Infecção
Leishmania
Dendritic Cells
Infection
dc.subject.other.en_US.fl_str_mv Leishmania
Células dendríticas
Infecção
dc.subject.en.en_US.fl_str_mv Leishmania
Dendritic Cells
Infection
description Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Interação Hospedeiro-Parasita e Epidemiologia. Salvador, BA, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-09-28T15:15:46Z
dc.date.available.fl_str_mv 2022-09-28T15:15:46Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PAIXÃO, Amanda R. et al. In vitro differentiation of human dendritic cells and their markers in leishmania infection. Journal of Visualized Experiments, v. 182, p. 1-14, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/54914
dc.identifier.issn.en_US.fl_str_mv 1940-087X
dc.identifier.doi.none.fl_str_mv 10.3791/62794
identifier_str_mv PAIXÃO, Amanda R. et al. In vitro differentiation of human dendritic cells and their markers in leishmania infection. Journal of Visualized Experiments, v. 182, p. 1-14, 2022.
1940-087X
10.3791/62794
url https://www.arca.fiocruz.br/handle/icict/54914
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MyJove Corporation
publisher.none.fl_str_mv MyJove Corporation
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/54914/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/54914/2/Paix%c3%a3o%2c%20Amanda%20-%20in%20vitro%20differentiation%20human.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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