Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2005 |
| Outros Autores: | , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/5962 |
Resumo: | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Leishmanioses e Vacinas. Belo Horizonte, MG, Brasil / The George Washington University. Washington, DC, USA. |
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Fujiwara, Ricardo ToshioVale, André MacedoSilva, João Carlos França daCosta, Roberto Teodoro daQuetz, Josiane da SilvaMartins Filho, Olindo AssisReis, Alexandre BarbosaOliveira, Rodrigo Corrêa deCoelho, George Luiz Lins MachadoBueno, Lilian LacerdaBethony, Jeffrey MichaelFrank, GlenNascimento, EvaldoGenaro, OdairMayrink, WilsonReed, Steven G.Campos Neto, Antonio2012-12-12T13:14:54Z2012-12-12T13:14:54Z2005FUJIWARA, Ricardo Toshio et al. Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis. Veterinary Research, v. 36, p. 827–838, 2005.0928-4249https://www.arca.fiocruz.br/handle/icict/596210.1051/vetres:2005033engBioMed CentralImmunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Leishmanioses e Vacinas. Belo Horizonte, MG, Brasil / The George Washington University. Washington, DC, USA.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Leishmanioses e Vacinas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Leishmanioses e Vacinas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Leishmanioses e Vacinas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Leishmanioses e Vacinas. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Doença de Chagas. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil / Universidade Federal de Ouro Preto. Escola de Farmácia. Ouro Preto, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil.Universidade Federal de Ouro Preto. Escola de Farmácia. Ouro Preto, MG, Brasil.The George Washington University. Washington, DC, USA.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil / The George Washington University. Washington, DC, USA.Heska Corporation. Fort Collins, CO, USA.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Leishmanioses e Vacinas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Leishmanioses e Vacinas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Leishmanioses e Vacinas. Belo Horizonte, MG, Brasil.The Infectious Disease Research Institute. Seattle, WA, USA.The Forsyth Institute. Boston, MA, USA.Control of canine visceral leishmaniasis (VL) remains a difficult and serious problem mostly because there is no reliable and effective vaccine available to prevent this disease. A mixture of three recombinant leishmanial antigens (TSA, LeIF and LmSTI1) encoded by three genes highly conserved in the Leishmania genus have been shown to induce excellent protection against infection in both murine and simian models of cutaneous leishmaniasis. A human clinical trial with these antigens is currently underway. Because of the high degree of conservation, these antigens might be useful vaccine candidates for VL as well. In the present study, using the dog model of the visceral disease, we evaluated the immunogenicity of these three antigens formulated with two different adjuvants, MPL-SE® and AdjuPrime®. The results were compared with a whole parasite vaccine formulated with BCG as the adjuvant. In order to investigate if sensitization with the recombinant antigens would result in recognition of the corresponding native parasite antigens upon infection, the animals were exposed for four weeks after the termination of the immunization protocol with the recombinant antigens to a low number of L. chagasi promastigotes, an etiological agent of VL. Immune response was evaluated by quantitative ELISA in the animal sera before and after exposure to the viable parasites. Both antigen specific IgG1 and IgG2 antibody levels were measured. Immunization of dogs with the recombinant antigens formulated in either MPL-SE® or AdjuPrime® resulted in high antibody levels particularly to LmSTI1. In addition, this immunization although to low levels, resulted in the development of antibody response to the whole parasite lysate. Importantly, experimental exposure with low numbers of culture forms of L. chagasi promastigotes caused a clear boost in the immune response to both the recombinant antigens and the corresponding native molecules. The boost response was predominantly of the IgG2 isotype in animals primed with the recombinant antigens plus MPL-SE®. In contrast, animals primed with the recombinant antigens formulated in AdjuPrime® as well as animals vaccinated with crude antigen preparation responded with mixed IgG1/IgG2 isotypes. These results point to the possible use of this antigen cocktail formulated with the adjuvant MPL-SE® in efficacy field trials against canine VL.Visceral leishmaniasisDogsVaccineLeishmania chagasiinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINAL27. Immunogenicity in dogs of three recombinant.pdf27. Immunogenicity in dogs of three recombinant.pdfapplication/pdf354129https://www.arca.fiocruz.br/bitstream/icict/5962/1/27.%20Immunogenicity%20in%20dogs%20of%20three%20recombinant.pdff4b66972c83cda316fff26bb9a4d34edMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81949https://www.arca.fiocruz.br/bitstream/icict/5962/2/license.txtaad6e5ff39a8b3723a1981fb3bcd1becMD52TEXT27. Immunogenicity in dogs of three recombinant.pdf.txt27. 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| dc.title.pt_BR.fl_str_mv |
Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis |
| title |
Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis |
| spellingShingle |
Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis Fujiwara, Ricardo Toshio Visceral leishmaniasis Dogs Vaccine Leishmania chagasi |
| title_short |
Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis |
| title_full |
Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis |
| title_fullStr |
Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis |
| title_full_unstemmed |
Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis |
| title_sort |
Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis |
| author |
Fujiwara, Ricardo Toshio |
| author_facet |
Fujiwara, Ricardo Toshio Vale, André Macedo Silva, João Carlos França da Costa, Roberto Teodoro da Quetz, Josiane da Silva Martins Filho, Olindo Assis Reis, Alexandre Barbosa Oliveira, Rodrigo Corrêa de Coelho, George Luiz Lins Machado Bueno, Lilian Lacerda Bethony, Jeffrey Michael Frank, Glen Nascimento, Evaldo Genaro, Odair Mayrink, Wilson Reed, Steven G. Campos Neto, Antonio |
| author_role |
author |
| author2 |
Vale, André Macedo Silva, João Carlos França da Costa, Roberto Teodoro da Quetz, Josiane da Silva Martins Filho, Olindo Assis Reis, Alexandre Barbosa Oliveira, Rodrigo Corrêa de Coelho, George Luiz Lins Machado Bueno, Lilian Lacerda Bethony, Jeffrey Michael Frank, Glen Nascimento, Evaldo Genaro, Odair Mayrink, Wilson Reed, Steven G. Campos Neto, Antonio |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Fujiwara, Ricardo Toshio Vale, André Macedo Silva, João Carlos França da Costa, Roberto Teodoro da Quetz, Josiane da Silva Martins Filho, Olindo Assis Reis, Alexandre Barbosa Oliveira, Rodrigo Corrêa de Coelho, George Luiz Lins Machado Bueno, Lilian Lacerda Bethony, Jeffrey Michael Frank, Glen Nascimento, Evaldo Genaro, Odair Mayrink, Wilson Reed, Steven G. Campos Neto, Antonio |
| dc.subject.en.pt_BR.fl_str_mv |
Visceral leishmaniasis Dogs Vaccine Leishmania chagasi |
| topic |
Visceral leishmaniasis Dogs Vaccine Leishmania chagasi |
| description |
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Leishmanioses e Vacinas. Belo Horizonte, MG, Brasil / The George Washington University. Washington, DC, USA. |
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2005 |
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2005 |
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2012-12-12T13:14:54Z |
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2012-12-12T13:14:54Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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FUJIWARA, Ricardo Toshio et al. Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis. Veterinary Research, v. 36, p. 827–838, 2005. |
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https://www.arca.fiocruz.br/handle/icict/5962 |
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0928-4249 |
| dc.identifier.doi.none.fl_str_mv |
10.1051/vetres:2005033 |
| identifier_str_mv |
FUJIWARA, Ricardo Toshio et al. Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis. Veterinary Research, v. 36, p. 827–838, 2005. 0928-4249 10.1051/vetres:2005033 |
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BioMed Central |
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BioMed Central |
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