Serological diagnosis of visceral leishmaniasis by an enzyme immunoassay using protein A in naturally infected dogs

Detalhes bibliográficos
Autor(a) principal: Lima,Valéria Marçal Felix de
Data de Publicação: 2005
Outros Autores: Biazzono,Luciane, Silva,Ana Cláudia, Correa,Ana Paula Ferreira Lopes, Luvizotto,Maria Cecília Rui
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2005000400005
Resumo: A rapid indirect enzyme-linked immunosorbent assay (ELISA) was developed for measuring antibodies against Leishmania chagasi using total antigen from lysed promastigotes. Fifty symptomatic mixed breed dogs from a region of high incidence of visceral leishmaniasis in Brazil were examined. The results showed that in the positive animals, diagnosed by cytological examination, the ELISA using protein A assay system (mean optical density ± SD / 2.078 ± 0.631) detected more antibodies than the anti-IgG assay (mean optical density ± SD / 1.008 ± 0.437), while in the negative animals, the results by both systems were similar. These results suggest that the ELISA assay using protein A peroxidase conjugated could be useful to detect early infected animals in endemic areas, and thus help to control the spread of the infection.
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spelling Serological diagnosis of visceral leishmaniasis by an enzyme immunoassay using protein A in naturally infected dogsLeishmania chagasivisceral leishmaniasisprotein AdogsA rapid indirect enzyme-linked immunosorbent assay (ELISA) was developed for measuring antibodies against Leishmania chagasi using total antigen from lysed promastigotes. Fifty symptomatic mixed breed dogs from a region of high incidence of visceral leishmaniasis in Brazil were examined. The results showed that in the positive animals, diagnosed by cytological examination, the ELISA using protein A assay system (mean optical density ± SD / 2.078 ± 0.631) detected more antibodies than the anti-IgG assay (mean optical density ± SD / 1.008 ± 0.437), while in the negative animals, the results by both systems were similar. These results suggest that the ELISA assay using protein A peroxidase conjugated could be useful to detect early infected animals in endemic areas, and thus help to control the spread of the infection.Colégio Brasileiro de Patologia Animal - CBPA2005-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2005000400005Pesquisa Veterinária Brasileira v.25 n.4 2005reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2005000400005info:eu-repo/semantics/openAccessLima,Valéria Marçal Felix deBiazzono,LucianeSilva,Ana CláudiaCorrea,Ana Paula Ferreira LopesLuvizotto,Maria Cecília Ruieng2006-02-01T00:00:00Zoai:scielo:S0100-736X2005000400005Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2006-02-01T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv Serological diagnosis of visceral leishmaniasis by an enzyme immunoassay using protein A in naturally infected dogs
title Serological diagnosis of visceral leishmaniasis by an enzyme immunoassay using protein A in naturally infected dogs
spellingShingle Serological diagnosis of visceral leishmaniasis by an enzyme immunoassay using protein A in naturally infected dogs
Lima,Valéria Marçal Felix de
Leishmania chagasi
visceral leishmaniasis
protein A
dogs
title_short Serological diagnosis of visceral leishmaniasis by an enzyme immunoassay using protein A in naturally infected dogs
title_full Serological diagnosis of visceral leishmaniasis by an enzyme immunoassay using protein A in naturally infected dogs
title_fullStr Serological diagnosis of visceral leishmaniasis by an enzyme immunoassay using protein A in naturally infected dogs
title_full_unstemmed Serological diagnosis of visceral leishmaniasis by an enzyme immunoassay using protein A in naturally infected dogs
title_sort Serological diagnosis of visceral leishmaniasis by an enzyme immunoassay using protein A in naturally infected dogs
author Lima,Valéria Marçal Felix de
author_facet Lima,Valéria Marçal Felix de
Biazzono,Luciane
Silva,Ana Cláudia
Correa,Ana Paula Ferreira Lopes
Luvizotto,Maria Cecília Rui
author_role author
author2 Biazzono,Luciane
Silva,Ana Cláudia
Correa,Ana Paula Ferreira Lopes
Luvizotto,Maria Cecília Rui
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Lima,Valéria Marçal Felix de
Biazzono,Luciane
Silva,Ana Cláudia
Correa,Ana Paula Ferreira Lopes
Luvizotto,Maria Cecília Rui
dc.subject.por.fl_str_mv Leishmania chagasi
visceral leishmaniasis
protein A
dogs
topic Leishmania chagasi
visceral leishmaniasis
protein A
dogs
description A rapid indirect enzyme-linked immunosorbent assay (ELISA) was developed for measuring antibodies against Leishmania chagasi using total antigen from lysed promastigotes. Fifty symptomatic mixed breed dogs from a region of high incidence of visceral leishmaniasis in Brazil were examined. The results showed that in the positive animals, diagnosed by cytological examination, the ELISA using protein A assay system (mean optical density ± SD / 2.078 ± 0.631) detected more antibodies than the anti-IgG assay (mean optical density ± SD / 1.008 ± 0.437), while in the negative animals, the results by both systems were similar. These results suggest that the ELISA assay using protein A peroxidase conjugated could be useful to detect early infected animals in endemic areas, and thus help to control the spread of the infection.
publishDate 2005
dc.date.none.fl_str_mv 2005-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2005000400005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2005000400005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-736X2005000400005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.25 n.4 2005
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
collection Pesquisa Veterinária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
repository.mail.fl_str_mv colegio@cbpa.org.br||pvb@pvb.com.br
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