Associations between systemic inflammation, mycobacterial loads in sputum and radiological improvement after treatment initiation in pulmonary TB patients from Brazil: a prospective cohort study

Detalhes bibliográficos
Autor(a) principal: Mesquita, Eliene Denites Duarte
Data de Publicação: 2016
Outros Autores: Gil-Santana, Leonardo, Ramalho, Daniela, Tonomura, Elise, Silva, Elisangela Costa da, Oliveira, Martha Maria de, Andrade, Bruno de Bezerril, Kritski, Afrânio Lineu
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16411
Resumo: CNPq/INCT 573548/2008-0; Faperj Process: Processo - E-26/110.974/2011.
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spelling Mesquita, Eliene Denites DuarteGil-Santana, LeonardoRamalho, DanielaTonomura, EliseSilva, Elisangela Costa daOliveira, Martha Maria deAndrade, Bruno de BezerrilKritski, Afrânio Lineu2016-12-01T17:15:35Z2016-12-01T17:15:35Z2016MESQUITA, E. D. D. et al. Associations between systemic inflammation, mycobacterial loads in sputum and radiological improvement after treatment initiation in pulmonary TB patients from Brazil: a prospective cohort study. BMC Infectious Diseases, v. 16, p. 368, 2016.1471-2334https://www.arca.fiocruz.br/handle/icict/1641110.1186/s12879-016-1736-3CNPq/INCT 573548/2008-0; Faperj Process: Processo - E-26/110.974/2011.Ary Parreira Institute. State Secretary of Health of Rio de Janeiro. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Integrado de Microbiologia e Imunorregulação (LIMI). Unidade de Medicina Investigativa. Salvador, BA, Brasil / Fundação José Silveira. Multinational Organization Network Sponsoring Translational and Epidemiological Research (MONSTER) Initiative. Salvador, BA, Brasil / Faculdade de Tecnologia e Ciências. School of Medicine. Salvador, BA, BrasilFederal University of Rio de Janeiro. School of Medicine. Tuberculosis Academic Program. Rio de Janeiro, RJ, BrasilFederal University of Rio de Janeiro. School of Medicine. Radiology Department. Rio de Janeiro, RJ, BrasilFederal University of Rio de Janeiro. School of Medicine. Tuberculosis Academic Program. Rio de Janeiro, RJ, Brasil / State University of North Fluminense Darcy Ribeiro. Recognize the Biology Laboratory. Center of Bioscience and Biotechnology. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Development Center for Technology on Health. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Integrado de Microbiologia e Imunorregulação (LIMI). Unidade de Medicina Investigativa. Salvador, BA, Brasil / Fundação José Silveira. Multinational Organization Network Sponsoring Translational and Epidemiological Research (MONSTER) Initiative. Salvador, BA, Brasil / Faculdade de Tecnologia e Ciências. School of Medicine. Salvador, BA, BrasilFederal University of Rio de Janeiro. School of Medicine. Tuberculosis Academic Program. Rio de Janeiro, RJ, BrasilRede-TB Study groupMycobacterium tuberculosis infection is known to cause inflammation and lung tissue damage in high-risk populations. Nevertheless, direct associations between mycobacterial loads, systemic inflammation and pulmonary lesions upon treatment initiation have not been fully characterized. In the present exploratory study, we prospectively depict the immune profile, microbial clearance and evolution of radiographic lesions in a pulmonary tuberculosis (PTB) patient cohort before and 60 days after anti-tuberculous treatment (ATT) initiation. Methods: Circulating levels of cytokines (IL-2, IL-4, IL-6, IL-10, IFN-γ, TNF-α) and C-reactive protein (CRP), as well as values of erythrocyte sedimentation rate (ESR) were measured in cryopreserved serum samples obtained from 73 PTB patients at pre-ATT and day 60 of treatment. Changes of the immune profile over time were compared with mycobacterial loads in sputum and culture conversion at day 60 of ATT. Additional analyses tested associations between improvement of chest radiographic lesions at day 60 and pre-treatment status of inflammation and mycobacterial loads. Results: Within the inflammatory parameters evaluated, values of CRP, IL-2, IL-4, TNF-α and ESR significantly decreased upon treatment initiation. On the converse, IL-10 levels substantially increased at day 60 of ATT, whereas concentrations of IL-6 and IFN-γ remained unchanged. Multidimensional analyses revealed that ESR, IL-2, IL-4 and CRP were the parameters with the highest power to discriminate individuals before and after treatment initiation. We further demonstrated that higher bacterial loads in sputum at pre-ATT were associated with increased systemic inflammation and higher risk for positive M. tuberculosis sputum cultures at day 60 of treatment. Furthermore, we found that pre-ATT mycobacterial loads in sputum and systemic inflammation synergistically associated with the status of radiographic lesions during treatment (Relative risk for chest X-ray improvement: 10.0, 95 % confidence interval: 2.4–40.0, P = 0.002). Conclusions: M. tuberculosis loads in sputum are directly associated to the status of systemic inflammation and potentially impact the immune profile, culture conversion and evolution of lung lesions upon ATT initiation.engBioMed CentralTuberculoseInflamaçãoProteína C-reativaBiomarcadorTaxa de sedimentação de eritrócitosBacilosTratamento antituberculosoAvaliação radiográficaTuberculosisInflammationC-reactive proteinBiomarkerErythrocyte sedimentation rateAcid-fast bacilliAnti-tuberculous treatmentRadiographic evaluationAssociations between systemic inflammation, mycobacterial loads in sputum and radiological improvement after treatment initiation in pulmonary TB patients from Brazil: a prospective cohort studyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Associations between systemic inflammation, mycobacterial loads in sputum and radiological improvement after treatment initiation in pulmonary TB patients from Brazil: a prospective cohort study
title Associations between systemic inflammation, mycobacterial loads in sputum and radiological improvement after treatment initiation in pulmonary TB patients from Brazil: a prospective cohort study
spellingShingle Associations between systemic inflammation, mycobacterial loads in sputum and radiological improvement after treatment initiation in pulmonary TB patients from Brazil: a prospective cohort study
Mesquita, Eliene Denites Duarte
Tuberculose
Inflamação
Proteína C-reativa
Biomarcador
Taxa de sedimentação de eritrócitos
Bacilos
Tratamento antituberculoso
Avaliação radiográfica
Tuberculosis
Inflammation
C-reactive protein
Biomarker
Erythrocyte sedimentation rate
Acid-fast bacilli
Anti-tuberculous treatment
Radiographic evaluation
title_short Associations between systemic inflammation, mycobacterial loads in sputum and radiological improvement after treatment initiation in pulmonary TB patients from Brazil: a prospective cohort study
title_full Associations between systemic inflammation, mycobacterial loads in sputum and radiological improvement after treatment initiation in pulmonary TB patients from Brazil: a prospective cohort study
title_fullStr Associations between systemic inflammation, mycobacterial loads in sputum and radiological improvement after treatment initiation in pulmonary TB patients from Brazil: a prospective cohort study
title_full_unstemmed Associations between systemic inflammation, mycobacterial loads in sputum and radiological improvement after treatment initiation in pulmonary TB patients from Brazil: a prospective cohort study
title_sort Associations between systemic inflammation, mycobacterial loads in sputum and radiological improvement after treatment initiation in pulmonary TB patients from Brazil: a prospective cohort study
author Mesquita, Eliene Denites Duarte
author_facet Mesquita, Eliene Denites Duarte
Gil-Santana, Leonardo
Ramalho, Daniela
Tonomura, Elise
Silva, Elisangela Costa da
Oliveira, Martha Maria de
Andrade, Bruno de Bezerril
Kritski, Afrânio Lineu
author_role author
author2 Gil-Santana, Leonardo
Ramalho, Daniela
Tonomura, Elise
Silva, Elisangela Costa da
Oliveira, Martha Maria de
Andrade, Bruno de Bezerril
Kritski, Afrânio Lineu
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mesquita, Eliene Denites Duarte
Gil-Santana, Leonardo
Ramalho, Daniela
Tonomura, Elise
Silva, Elisangela Costa da
Oliveira, Martha Maria de
Andrade, Bruno de Bezerril
Kritski, Afrânio Lineu
dc.subject.other.pt_BR.fl_str_mv Tuberculose
Inflamação
Proteína C-reativa
Biomarcador
Taxa de sedimentação de eritrócitos
Bacilos
Tratamento antituberculoso
Avaliação radiográfica
topic Tuberculose
Inflamação
Proteína C-reativa
Biomarcador
Taxa de sedimentação de eritrócitos
Bacilos
Tratamento antituberculoso
Avaliação radiográfica
Tuberculosis
Inflammation
C-reactive protein
Biomarker
Erythrocyte sedimentation rate
Acid-fast bacilli
Anti-tuberculous treatment
Radiographic evaluation
dc.subject.en.pt_BR.fl_str_mv Tuberculosis
Inflammation
C-reactive protein
Biomarker
Erythrocyte sedimentation rate
Acid-fast bacilli
Anti-tuberculous treatment
Radiographic evaluation
description CNPq/INCT 573548/2008-0; Faperj Process: Processo - E-26/110.974/2011.
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-12-01T17:15:35Z
dc.date.available.fl_str_mv 2016-12-01T17:15:35Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv MESQUITA, E. D. D. et al. Associations between systemic inflammation, mycobacterial loads in sputum and radiological improvement after treatment initiation in pulmonary TB patients from Brazil: a prospective cohort study. BMC Infectious Diseases, v. 16, p. 368, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16411
dc.identifier.issn.pt_BR.fl_str_mv 1471-2334
dc.identifier.doi.none.fl_str_mv 10.1186/s12879-016-1736-3
identifier_str_mv MESQUITA, E. D. D. et al. Associations between systemic inflammation, mycobacterial loads in sputum and radiological improvement after treatment initiation in pulmonary TB patients from Brazil: a prospective cohort study. BMC Infectious Diseases, v. 16, p. 368, 2016.
1471-2334
10.1186/s12879-016-1736-3
url https://www.arca.fiocruz.br/handle/icict/16411
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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