Cohort study of molecular identification and typing of Mycobacterium abscessus, Mycobacterium massiliense, and Mycobacterium bolletii

Detalhes bibliográficos
Autor(a) principal: Zelazny, Adrian M.
Data de Publicação: 2009
Outros Autores: Root, Jeremy M., Shea, Yvonne R., Colombo, Rhonda E., Shamputa, Isdore C., Stock, Frida, Conlan, Sean, McNulty, Steven, Brown-Elliott, Barbara A., Wallace, Richard J., Olivier, Kenneth N., Holland, Steven M., Sampaio, Elizabeth P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/29054
Resumo: National Institutes of Health. Clinical Center. Department of Laboratory Medicine. Microbiology Service. Bethesda, Maryland, USA / National Institutes of Health. Clinical Center. Department of Laboratory Medicine. Immunopathogenesis Section. Bethesda, Maryland, USA.
id CRUZ_feba28dda46938d26814e6e603338412
oai_identifier_str oai:www.arca.fiocruz.br:icict/29054
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Zelazny, Adrian M.Root, Jeremy M.Shea, Yvonne R.Colombo, Rhonda E.Shamputa, Isdore C.Stock, FridaConlan, SeanMcNulty, StevenBrown-Elliott, Barbara A.Wallace, Richard J.Olivier, Kenneth N.Holland, Steven M.Sampaio, Elizabeth P.2018-09-25T18:04:55Z2018-09-25T18:04:55Z2009ZELAZNY, Adrian M. et al. Cohort Study of Molecular Identification and Typing of Mycobacterium abscessus, Mycobacterium massiliense, and Mycobacterium bolletii. Journal of Clinical Microbiology, v.47, n.7, p.1985-1995, July 2009.0095-1137https://www.arca.fiocruz.br/handle/icict/2905410.1128/JCM.01688-081098-660XengAmerican Society for Microbiologyidentificação molecularestudo de coorteMycobacterium abscessusMycobacterium massilienseMycobacterium bolletiidoença pulmonarCohort Studyestudo de coorteMycobacterium abscessusMycobacterium massilienseMycobacterium bolletiilung diseaseCohort study of molecular identification and typing of Mycobacterium abscessus, Mycobacterium massiliense, and Mycobacterium bolletiiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNational Institutes of Health. Clinical Center. Department of Laboratory Medicine. Microbiology Service. Bethesda, Maryland, USA / National Institutes of Health. Clinical Center. Department of Laboratory Medicine. Immunopathogenesis Section. Bethesda, Maryland, USA.National Institutes of Health. Clinical Center. Department of Laboratory Medicine. Immunopathogenesis Section. Bethesda, Maryland, USA.National Institutes of Health. Clinical Center. Department of Laboratory Medicine. Microbiology Service. Bethesda, Maryland, USANational Institutes of Health. Clinical Center. Department of Laboratory Medicine. Immunopathogenesis Section. Bethesda, Maryland, USA.National Institutes of Health. Clinical Center. Department of Laboratory Medicine. Tuberculosis Research Section. Bethesda, Maryland, USA.National Institutes of Health. Clinical Center. Department of Laboratory Medicine. Microbiology Service. Bethesda, Maryland, USANational Institutes of Health. National Human Genome Research Institute. Bethesda, Maryland, USA.The University of Texas Health Science Center. Department of Microbiology. Tyler, Texas, USA.The University of Texas Health Science Center. Department of Microbiology. Tyler, Texas, USA.The University of Texas Health Science Center. Department of Microbiology. Tyler, Texas, USA.National Institutes of Health. Clinical Center. Department of Laboratory Medicine. Immunopathogenesis Section. Bethesda, Maryland, USA.National Institutes of Health. Clinical Center. Department of Laboratory Medicine. Immunopathogenesis Section. Bethesda, Maryland, USA / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Mycobacterium abscessus is the most common cause of rapidly growing mycobacterial chronic lung disease. Recently, two new M. abscessus-related species, M. massiliense and M. bolletii, have been described. Health care-associated outbreaks have recently been investigated by the use of molecular identification and typing tools; however, very little is known about the natural epidemiology and pathogenicity of M. massiliense or M. bolletii outside of outbreak situations. The differentiation of these two species from M. abscessus is difficult and relies on the sequencing of one or more housekeeping genes. We performed extensive molecular identification and typing of 42 clinical isolates of M. abscessus, M. massiliense, and M. bolletii from patients monitored at the NIH between 1999 and 2007. The corresponding clinical data were also examined. Partial sequencing of rpoB, hsp65, and secA led to the unambiguous identification of 26 M. abscessus isolates, 7 M. massiliense isolates, and 2 M. bolletii isolates. The identification results for seven other isolates were ambiguous and warranted further sequencing and an integrated phylogenetic analysis. Strain relatedness was assessed by repetitive-sequence-based PCR (rep-PCR) and pulsed-field gel electrophoresis (PFGE), which showed the characteristic clonal groups for each species. Five isolates with ambiguous species identities as M. abscessus-M. massiliense by rpoB, hsp65, and secA sequencing clustered as a distinct group by rep-PCR and PFGE together with the M. massiliense type strain. Overall, the clinical manifestations of disease caused by each species were similar. In summary, a multilocus sequencing approach (not just rpoB partial sequencing) is required for division of M. abscessus and closely related species. Molecular typing complements sequence-based identification and provides information on prevalent clones with possible relevant clinical aspects.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALelisabeth2_sampaio_etal_IOC_2009.pdfelisabeth2_sampaio_etal_IOC_2009.pdfapplication/pdf1445529https://www.arca.fiocruz.br/bitstream/icict/29054/2/elisabeth2_sampaio_etal_IOC_2009.pdf8222b19cc6edf5b2e7fcb87dfd6309bdMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/29054/3/license.txt5a560609d32a3863062d77ff32785d58MD53TEXTelisabeth2_sampaio_etal_IOC_2009.pdf.txtelisabeth2_sampaio_etal_IOC_2009.pdf.txtExtracted texttext/plain49262https://www.arca.fiocruz.br/bitstream/icict/29054/4/elisabeth2_sampaio_etal_IOC_2009.pdf.txt96f08316cc61898de87a272048d6fb1bMD54icict/290542018-09-26 02:01:36.915oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-09-26T05:01:36Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Cohort study of molecular identification and typing of Mycobacterium abscessus, Mycobacterium massiliense, and Mycobacterium bolletii
title Cohort study of molecular identification and typing of Mycobacterium abscessus, Mycobacterium massiliense, and Mycobacterium bolletii
spellingShingle Cohort study of molecular identification and typing of Mycobacterium abscessus, Mycobacterium massiliense, and Mycobacterium bolletii
Zelazny, Adrian M.
identificação molecular
estudo de coorte
Mycobacterium abscessus
Mycobacterium massiliense
Mycobacterium bolletii
doença pulmonar
Cohort Study
estudo de coorte
Mycobacterium abscessus
Mycobacterium massiliense
Mycobacterium bolletii
lung disease
title_short Cohort study of molecular identification and typing of Mycobacterium abscessus, Mycobacterium massiliense, and Mycobacterium bolletii
title_full Cohort study of molecular identification and typing of Mycobacterium abscessus, Mycobacterium massiliense, and Mycobacterium bolletii
title_fullStr Cohort study of molecular identification and typing of Mycobacterium abscessus, Mycobacterium massiliense, and Mycobacterium bolletii
title_full_unstemmed Cohort study of molecular identification and typing of Mycobacterium abscessus, Mycobacterium massiliense, and Mycobacterium bolletii
title_sort Cohort study of molecular identification and typing of Mycobacterium abscessus, Mycobacterium massiliense, and Mycobacterium bolletii
author Zelazny, Adrian M.
author_facet Zelazny, Adrian M.
Root, Jeremy M.
Shea, Yvonne R.
Colombo, Rhonda E.
Shamputa, Isdore C.
Stock, Frida
Conlan, Sean
McNulty, Steven
Brown-Elliott, Barbara A.
Wallace, Richard J.
Olivier, Kenneth N.
Holland, Steven M.
Sampaio, Elizabeth P.
author_role author
author2 Root, Jeremy M.
Shea, Yvonne R.
Colombo, Rhonda E.
Shamputa, Isdore C.
Stock, Frida
Conlan, Sean
McNulty, Steven
Brown-Elliott, Barbara A.
Wallace, Richard J.
Olivier, Kenneth N.
Holland, Steven M.
Sampaio, Elizabeth P.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Zelazny, Adrian M.
Root, Jeremy M.
Shea, Yvonne R.
Colombo, Rhonda E.
Shamputa, Isdore C.
Stock, Frida
Conlan, Sean
McNulty, Steven
Brown-Elliott, Barbara A.
Wallace, Richard J.
Olivier, Kenneth N.
Holland, Steven M.
Sampaio, Elizabeth P.
dc.subject.other.pt_BR.fl_str_mv identificação molecular
estudo de coorte
Mycobacterium abscessus
Mycobacterium massiliense
Mycobacterium bolletii
doença pulmonar
topic identificação molecular
estudo de coorte
Mycobacterium abscessus
Mycobacterium massiliense
Mycobacterium bolletii
doença pulmonar
Cohort Study
estudo de coorte
Mycobacterium abscessus
Mycobacterium massiliense
Mycobacterium bolletii
lung disease
dc.subject.en.pt_BR.fl_str_mv Cohort Study
estudo de coorte
Mycobacterium abscessus
Mycobacterium massiliense
Mycobacterium bolletii
lung disease
description National Institutes of Health. Clinical Center. Department of Laboratory Medicine. Microbiology Service. Bethesda, Maryland, USA / National Institutes of Health. Clinical Center. Department of Laboratory Medicine. Immunopathogenesis Section. Bethesda, Maryland, USA.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2018-09-25T18:04:55Z
dc.date.available.fl_str_mv 2018-09-25T18:04:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ZELAZNY, Adrian M. et al. Cohort Study of Molecular Identification and Typing of Mycobacterium abscessus, Mycobacterium massiliense, and Mycobacterium bolletii. Journal of Clinical Microbiology, v.47, n.7, p.1985-1995, July 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/29054
dc.identifier.issn.pt_BR.fl_str_mv 0095-1137
dc.identifier.doi.none.fl_str_mv 10.1128/JCM.01688-08
dc.identifier.eissn.none.fl_str_mv 1098-660X
identifier_str_mv ZELAZNY, Adrian M. et al. Cohort Study of Molecular Identification and Typing of Mycobacterium abscessus, Mycobacterium massiliense, and Mycobacterium bolletii. Journal of Clinical Microbiology, v.47, n.7, p.1985-1995, July 2009.
0095-1137
10.1128/JCM.01688-08
1098-660X
url https://www.arca.fiocruz.br/handle/icict/29054
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/29054/2/elisabeth2_sampaio_etal_IOC_2009.pdf
https://www.arca.fiocruz.br/bitstream/icict/29054/3/license.txt
https://www.arca.fiocruz.br/bitstream/icict/29054/4/elisabeth2_sampaio_etal_IOC_2009.pdf.txt
bitstream.checksum.fl_str_mv 8222b19cc6edf5b2e7fcb87dfd6309bd
5a560609d32a3863062d77ff32785d58
96f08316cc61898de87a272048d6fb1b
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324864319225856

Registros relacionados