Phenotypic and molecular characterization of quinolone resistance in Mycobacterium abscessus subsp. bolletii recovered from postsurgical infections

Detalhes bibliográficos
Autor(a) principal: Moura, Vinicius Calado Nogueira de
Data de Publicação: 2012
Outros Autores: Silva, Marlei Gomes da, Gomes, Karen Machado, Coelho, Fábrice Santana, Sampaio, Jorge Luiz Mello, Mello, Fernanda Carvalho de Queiroz, Lourenço, Maria Cristina da Silva, Amorim, Efigênia de Lourdes Teixeira, Duarte, Rafael Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36591
Resumo: 2020-10-21
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spelling Moura, Vinicius Calado Nogueira deSilva, Marlei Gomes daGomes, Karen MachadoCoelho, Fábrice SantanaSampaio, Jorge Luiz MelloMello, Fernanda Carvalho de QueirozLourenço, Maria Cristina da SilvaAmorim, Efigênia de Lourdes TeixeiraDuarte, Rafael Silva2019-10-21T12:40:05Z2019-10-21T12:40:05Z2012MOURA, Vinicius Calado Nogueira de et al. Phenotypic and molecular characterization of quinolone resistance in Mycobacterium abscessus subsp. bolletii recovered from postsurgical infections. Journal of Medical Microbiology, v. 61, p. 115-125, 2012.0022-2615https://www.arca.fiocruz.br/handle/icict/3659110.1099/jmm.0.034942-01473-5644engMicrobiology SocietyPhenotypic and molecular characterization of quinolone resistance in Mycobacterium abscessus subsp. bolletii recovered from postsurgical infectionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-10-21Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.Hospital Universitário Pedro Ernesto. Rio de Janeiro, RJ, Brasil.Instituto Fleury de Ensino e Pesquisa. São Paulo, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Doenças do Tórax. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Evandro Chagas. Rio de Janeiro, RJ, Brasil.Sérgio Franco Medicina Diagnóstica. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.Several outbreaks of infections caused by rapidly growing mycobacteria (RGM) were reported in many Brazilian states (2032 notified cases) from 2004 to 2010. Most of the confirmed cases were mainly associated with Mycobacterium massiliense (recently renamed as Mycobacterium abscessus subsp. bolletii) BRA100 clone, recovered from patients who had undergone invasive procedures in which medical instruments had not been properly sterilized and/or disinfected. Since quinolones have been an option for the treatment of general RGM infections and have been suggested for therapeutic schemes for these outbreaks, we evaluated the in vitro activities of all generations of quinolones for clinical and reference RGM by broth microdilution, and analysed the peptide sequences of the quinolone resistance determining regions (QRDRs) of GyrA and GyrB after DNA sequencing followed by amino acid translation. Fifty-four isolates of M. abscessus subsp. bolletii, including clone BRA100, recovered in different states of Brazil, and 19 reference strains of RGM species were characterized. All 54 M. abscessus subsp. bolletii isolates were resistant to all generations of quinolones and showed the same amino acids in the QRDRs, including the Ala-83 in GyrA, and Arg-447 and Asp-464 in GyrB, described as being responsible for an intrinsic low level of resistance to quinolones in mycobacteria. However, other RGM species showed distinct susceptibilities to this class of antimicrobials and patterns of mutations contrary to what has been traditionally defined, suggesting that other mechanisms of resistance, different from gyrA or gyrB mutations, may also be involved in resistance to high levels of quinolones.Mycobacterium abscessus subsp. bolletiiQuinolonePostsurgical infectionsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/36591/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Moura_Vinicius_etal_INI_2012.pdfve_Moura_Vinicius_etal_INI_2012.pdfapplication/pdf746076https://www.arca.fiocruz.br/bitstream/icict/36591/2/ve_Moura_Vinicius_etal_INI_2012.pdf374d3f4420810b1143a189824f7e8566MD52TEXTve_Moura_Vinicius_etal_INI_2012.pdf.txtve_Moura_Vinicius_etal_INI_2012.pdf.txtExtracted texttext/plain42802https://www.arca.fiocruz.br/bitstream/icict/36591/3/ve_Moura_Vinicius_etal_INI_2012.pdf.txt721357c59297a7a4828929b9ac17cc97MD53icict/365912021-03-24 16:32:37.933oai:www.arca.fiocruz.br:icict/36591Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:37Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Phenotypic and molecular characterization of quinolone resistance in Mycobacterium abscessus subsp. bolletii recovered from postsurgical infections
title Phenotypic and molecular characterization of quinolone resistance in Mycobacterium abscessus subsp. bolletii recovered from postsurgical infections
spellingShingle Phenotypic and molecular characterization of quinolone resistance in Mycobacterium abscessus subsp. bolletii recovered from postsurgical infections
Moura, Vinicius Calado Nogueira de
Mycobacterium abscessus subsp. bolletii
Quinolone
Postsurgical infections
title_short Phenotypic and molecular characterization of quinolone resistance in Mycobacterium abscessus subsp. bolletii recovered from postsurgical infections
title_full Phenotypic and molecular characterization of quinolone resistance in Mycobacterium abscessus subsp. bolletii recovered from postsurgical infections
title_fullStr Phenotypic and molecular characterization of quinolone resistance in Mycobacterium abscessus subsp. bolletii recovered from postsurgical infections
title_full_unstemmed Phenotypic and molecular characterization of quinolone resistance in Mycobacterium abscessus subsp. bolletii recovered from postsurgical infections
title_sort Phenotypic and molecular characterization of quinolone resistance in Mycobacterium abscessus subsp. bolletii recovered from postsurgical infections
author Moura, Vinicius Calado Nogueira de
author_facet Moura, Vinicius Calado Nogueira de
Silva, Marlei Gomes da
Gomes, Karen Machado
Coelho, Fábrice Santana
Sampaio, Jorge Luiz Mello
Mello, Fernanda Carvalho de Queiroz
Lourenço, Maria Cristina da Silva
Amorim, Efigênia de Lourdes Teixeira
Duarte, Rafael Silva
author_role author
author2 Silva, Marlei Gomes da
Gomes, Karen Machado
Coelho, Fábrice Santana
Sampaio, Jorge Luiz Mello
Mello, Fernanda Carvalho de Queiroz
Lourenço, Maria Cristina da Silva
Amorim, Efigênia de Lourdes Teixeira
Duarte, Rafael Silva
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Moura, Vinicius Calado Nogueira de
Silva, Marlei Gomes da
Gomes, Karen Machado
Coelho, Fábrice Santana
Sampaio, Jorge Luiz Mello
Mello, Fernanda Carvalho de Queiroz
Lourenço, Maria Cristina da Silva
Amorim, Efigênia de Lourdes Teixeira
Duarte, Rafael Silva
dc.subject.en.pt_BR.fl_str_mv Mycobacterium abscessus subsp. bolletii
Quinolone
Postsurgical infections
topic Mycobacterium abscessus subsp. bolletii
Quinolone
Postsurgical infections
description 2020-10-21
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2019-10-21T12:40:05Z
dc.date.available.fl_str_mv 2019-10-21T12:40:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MOURA, Vinicius Calado Nogueira de et al. Phenotypic and molecular characterization of quinolone resistance in Mycobacterium abscessus subsp. bolletii recovered from postsurgical infections. Journal of Medical Microbiology, v. 61, p. 115-125, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36591
dc.identifier.issn.pt_BR.fl_str_mv 0022-2615
dc.identifier.doi.none.fl_str_mv 10.1099/jmm.0.034942-0
dc.identifier.eissn.none.fl_str_mv 1473-5644
identifier_str_mv MOURA, Vinicius Calado Nogueira de et al. Phenotypic and molecular characterization of quinolone resistance in Mycobacterium abscessus subsp. bolletii recovered from postsurgical infections. Journal of Medical Microbiology, v. 61, p. 115-125, 2012.
0022-2615
10.1099/jmm.0.034942-0
1473-5644
url https://www.arca.fiocruz.br/handle/icict/36591
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Microbiology Society
publisher.none.fl_str_mv Microbiology Society
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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