The antioxidant role of xanthurenic acid in the Aedes aegypti midgut during digestion of a blood meal

Detalhes bibliográficos
Autor(a) principal: Lima, Vitor L. A.
Data de Publicação: 2012
Outros Autores: Dias, Felipe, Nunes, Rodrigo D., Pereira, Luiza O., Santos, Tiago S. R., Chiarini, Luciana B., Ramos, Tadeu D., Mendes, Bernardo J. Silva, Perales, Jonas, Valente, Richard H., Oliveira, Pedro L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18757
Resumo: Universidade Federal de Alagoas. Instituto de Química e Biotecnologia. Maceió, AL, Brasil.
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spelling Lima, Vitor L. A.Dias, FelipeNunes, Rodrigo D.Pereira, Luiza O.Santos, Tiago S. R.Chiarini, Luciana B.Ramos, Tadeu D.Mendes, Bernardo J. SilvaPerales, JonasValente, Richard H.Oliveira, Pedro L.2017-05-04T15:54:22Z2017-05-04T15:54:22Z2012LIMA, Vitor L. A. et al. The Antioxidant Role of Xanthurenic Acid in the Aedes aegypti Midgut during Digestion of a Blood Meal. Plos One, v.7, n.6, e38349, 8p, June 2012.1932-6203https://www.arca.fiocruz.br/handle/icict/1875710.1371/journal.pone.0038349engPublic Library of ScienceAedes aegyptiAntioxidantesTriptofanoDigestãoAedes aegyptiXanthurenic AcidAntioxidant RoleDigestionblood mealTryptophanThe antioxidant role of xanthurenic acid in the Aedes aegypti midgut during digestion of a blood mealinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Alagoas. Instituto de Química e Biotecnologia. Maceió, AL, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Programa de Biologia Molecular e Biotecnologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Programa de Biologia Molecular e Biotecnologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Me´dicas. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Programa de Biologia Molecular e Biotecnologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro, Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Programa de Biologia Molecular e Biotecnologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Programa de Biologia Molecular e Biotecnologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Programa de Biologia Molecular e Biotecnologia. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular, Rio de Janeiro, RJ, Brasil.In the midgut of the mosquito Aedes aegypti, a vector of dengue and yellow fever, an intense release of heme and iron takes place during the digestion of a blood meal. Here, we demonstrated via chromatography, light absorption and mass spectrometry that xanthurenic acid (XA), a product of the oxidative metabolism of tryptophan, is produced in the digestive apparatus after the ingestion of a blood meal and reaches milimolar levels after 24 h, the period of maximal digestive activity. XA formation does not occur in the White Eye (WE) strain, which lacks kynurenine hydroxylase and accumulates kynurenic acid. The formation of XA can be diminished by feeding the insect with 3,4-dimethoxy-N-[4-(3-nitrophenyl)thiazol-2-yl] benzenesulfonamide (Ro-61-8048), an inhibitor of XA biosynthesis. Moreover, XA inhibits the phospholipid oxidation induced by heme or iron. A major fraction of this antioxidant activity is due to the capacity of XA to bind both heme and iron, which occurs at a slightly alkaline pH (7.5-8.0), a condition found in the insect midgut. The midgut epithelial cells of the WE mosquito has a marked increase in occurrence of cell death, which is reversed to levels similar to the wild type mosquitoes by feeding the insects with blood supplemented with XA, confirming the protective role of this molecule. Collectively, these results suggest a new role for XA as a heme and iron chelator that provides protection as an antioxidant and may help these animals adapt to a blood feeding habit.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18757/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALluiza_pereira_etal_IOC_2012.pdfluiza_pereira_etal_IOC_2012.pdfapplication/pdf2344997https://www.arca.fiocruz.br/bitstream/icict/18757/2/luiza_pereira_etal_IOC_2012.pdf4c85a98ab80cc9f46d9c96570ee08a52MD52TEXTluiza_pereira_etal_IOC_2012.pdf.txtluiza_pereira_etal_IOC_2012.pdf.txtExtracted texttext/plain38306https://www.arca.fiocruz.br/bitstream/icict/18757/3/luiza_pereira_etal_IOC_2012.pdf.txt807de5350903d301375faf74df997b23MD53icict/187572023-09-14 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dc.title.pt_BR.fl_str_mv The antioxidant role of xanthurenic acid in the Aedes aegypti midgut during digestion of a blood meal
title The antioxidant role of xanthurenic acid in the Aedes aegypti midgut during digestion of a blood meal
spellingShingle The antioxidant role of xanthurenic acid in the Aedes aegypti midgut during digestion of a blood meal
Lima, Vitor L. A.
Aedes aegypti
Antioxidantes
Triptofano
Digestão
Aedes aegypti
Xanthurenic Acid
Antioxidant Role
Digestion
blood meal
Tryptophan
title_short The antioxidant role of xanthurenic acid in the Aedes aegypti midgut during digestion of a blood meal
title_full The antioxidant role of xanthurenic acid in the Aedes aegypti midgut during digestion of a blood meal
title_fullStr The antioxidant role of xanthurenic acid in the Aedes aegypti midgut during digestion of a blood meal
title_full_unstemmed The antioxidant role of xanthurenic acid in the Aedes aegypti midgut during digestion of a blood meal
title_sort The antioxidant role of xanthurenic acid in the Aedes aegypti midgut during digestion of a blood meal
author Lima, Vitor L. A.
author_facet Lima, Vitor L. A.
Dias, Felipe
Nunes, Rodrigo D.
Pereira, Luiza O.
Santos, Tiago S. R.
Chiarini, Luciana B.
Ramos, Tadeu D.
Mendes, Bernardo J. Silva
Perales, Jonas
Valente, Richard H.
Oliveira, Pedro L.
author_role author
author2 Dias, Felipe
Nunes, Rodrigo D.
Pereira, Luiza O.
Santos, Tiago S. R.
Chiarini, Luciana B.
Ramos, Tadeu D.
Mendes, Bernardo J. Silva
Perales, Jonas
Valente, Richard H.
Oliveira, Pedro L.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lima, Vitor L. A.
Dias, Felipe
Nunes, Rodrigo D.
Pereira, Luiza O.
Santos, Tiago S. R.
Chiarini, Luciana B.
Ramos, Tadeu D.
Mendes, Bernardo J. Silva
Perales, Jonas
Valente, Richard H.
Oliveira, Pedro L.
dc.subject.other.pt_BR.fl_str_mv Aedes aegypti
Antioxidantes
Triptofano
Digestão
topic Aedes aegypti
Antioxidantes
Triptofano
Digestão
Aedes aegypti
Xanthurenic Acid
Antioxidant Role
Digestion
blood meal
Tryptophan
dc.subject.en.pt_BR.fl_str_mv Aedes aegypti
Xanthurenic Acid
Antioxidant Role
Digestion
blood meal
Tryptophan
description Universidade Federal de Alagoas. Instituto de Química e Biotecnologia. Maceió, AL, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2017-05-04T15:54:22Z
dc.date.available.fl_str_mv 2017-05-04T15:54:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LIMA, Vitor L. A. et al. The Antioxidant Role of Xanthurenic Acid in the Aedes aegypti Midgut during Digestion of a Blood Meal. Plos One, v.7, n.6, e38349, 8p, June 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18757
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0038349
identifier_str_mv LIMA, Vitor L. A. et al. The Antioxidant Role of Xanthurenic Acid in the Aedes aegypti Midgut during Digestion of a Blood Meal. Plos One, v.7, n.6, e38349, 8p, June 2012.
1932-6203
10.1371/journal.pone.0038349
url https://www.arca.fiocruz.br/handle/icict/18757
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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