Pastas endodônticas formuladas com óleos vegetais: ação na microbiota bucal e na formação de ponte dentinária em cães

Detalhes bibliográficos
Autor(a) principal: Dias, Fernanda Gosuen Gonçalves
Data de Publicação: 2011
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório do Centro Universitário Braz Cubas
Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/695
Resumo: This study evaluated the action of endodontics pastes formulated with calcium hydroxide P.A. and different vehicles (physiological solution – paste A, Pterodon emarginatus Vogel oil - paste B and Copaifera langsdorffii Desfon oil - paste C) against oral microorganisms, and on bridge dentin formation in dogs. The antimicrobial action of the pastes and their individual components were evaluated by the technique of determining the minimum inhibitory concentration (MIC) by agar dilution. The materials were diluted at concentrations of 0,5%, 1,0%, 2,0% and 4,0%, and evaluated against fifteen standard strains of aerobic, microaerophilic, anaerobic and facultative anaerobes microorganisms that are related to endodontic diseases. The microrganisms were grown in ideal conditions and, after incubation, the data were obtained and analyzed (Anova One-Way, Tukey, p≤0,05). For the assessment of dentin bridge formation, six upper incisors of ten animals were treated, being the right side and the middle capped with paste A (Group A), the central right and left with paste B (Group B) and the medial and left lateral side teeth with paste C (Group C). After 90 days, the teeth were extracted to obtain histological sections, with the objective of evaluating the degree of dentin bridge formation. The data were subjected to statistical analysis (Kruskal-Wallis, p≤0,05). According to the antimicrobial action, the materials were classified in the following decrescent order: calcium hydroxide P.A., paste A, paste C, paste B, Pterodon emarginatus Vogel oil, and Copaifera langsdorffii Desfon oil, with statistically significant differences between the hydroxide calcium P.A., pastes, and vehicle (p ≤ 0,05). At the end of experimental time, it was observed that the three pastes were similar in relation to the formation of dentin bridge. Considering the microorganisms selected for this study, it was concluded that the pastes were similar in terms of antimicrobial potential, differing from the individual components (p≤0,05). Regarding to the formation of dentin bridge, at the end of experimental period, there were no significant differences between the teeth treated with different pastes.
id CUB_bf8e10be29a0d37e9833a082008a3fb4
oai_identifier_str oai:repositorio.cruzeirodosul.edu.br:123456789/695
network_acronym_str CUB
network_name_str Repositório do Centro Universitário Braz Cubas
repository_id_str
spelling Pastas endodônticas formuladas com óleos vegetais: ação na microbiota bucal e na formação de ponte dentinária em cãesEndodontics pastes formulated with vegetable oils: action on oral microbiota and on dentin bridge formation in dogsEndodontia - CãesMicrobiologiaCapeamento da polpa dentáriaPlantas medicinaisCNPQ::CIENCIAS AGRARIAS::ZOOTECNIAThis study evaluated the action of endodontics pastes formulated with calcium hydroxide P.A. and different vehicles (physiological solution – paste A, Pterodon emarginatus Vogel oil - paste B and Copaifera langsdorffii Desfon oil - paste C) against oral microorganisms, and on bridge dentin formation in dogs. The antimicrobial action of the pastes and their individual components were evaluated by the technique of determining the minimum inhibitory concentration (MIC) by agar dilution. The materials were diluted at concentrations of 0,5%, 1,0%, 2,0% and 4,0%, and evaluated against fifteen standard strains of aerobic, microaerophilic, anaerobic and facultative anaerobes microorganisms that are related to endodontic diseases. The microrganisms were grown in ideal conditions and, after incubation, the data were obtained and analyzed (Anova One-Way, Tukey, p≤0,05). For the assessment of dentin bridge formation, six upper incisors of ten animals were treated, being the right side and the middle capped with paste A (Group A), the central right and left with paste B (Group B) and the medial and left lateral side teeth with paste C (Group C). After 90 days, the teeth were extracted to obtain histological sections, with the objective of evaluating the degree of dentin bridge formation. The data were subjected to statistical analysis (Kruskal-Wallis, p≤0,05). According to the antimicrobial action, the materials were classified in the following decrescent order: calcium hydroxide P.A., paste A, paste C, paste B, Pterodon emarginatus Vogel oil, and Copaifera langsdorffii Desfon oil, with statistically significant differences between the hydroxide calcium P.A., pastes, and vehicle (p ≤ 0,05). At the end of experimental time, it was observed that the three pastes were similar in relation to the formation of dentin bridge. Considering the microorganisms selected for this study, it was concluded that the pastes were similar in terms of antimicrobial potential, differing from the individual components (p≤0,05). Regarding to the formation of dentin bridge, at the end of experimental period, there were no significant differences between the teeth treated with different pastes.Esse trabalho avaliou a ação de pastas endodônticas formuladas com hidróxido de cálcio P.A. e diferentes veículos (solução fisiológica – pasta A, óleo de Pterodon emarginatus Vogel – pasta B e óleo de Copaifera langsdorffii Desfon – pasta C) frente a microrganismos bucais e na formação de ponte dentinária em cães. A ação antimicrobiana das pastas e de seus componentes individuais foi avaliada pela técnica da determinação da concentração inibitória mínima (CIM) pela diluição em ágar. Os materiais foram diluídos nas concentrações de 0,5%, 1,0%, 2,0% e 4,0% e avaliados frente a quinze cepas padrão de microrganismos aeróbios, microaerófilos, anaeróbios e anaeróbios facultativos relacionados a doenças endodônticas. Os microrganismos foram cultivados em condições ideais e, após a incubação, os dados foram obtidos e analisados (Anova One- Way, Tukey, p≤0,05). Para a avaliação da formação de ponte dentinária, seis incisivos superiores de dez animais foram tratados, sendo os laterais e médios direitos capeados com pasta A (Grupo A), os centrais direito e esquerdo com pasta B (Grupo B) e os dentes médios e laterais esquerdos com pasta C (Grupo C). Após 90 dias, os dentes foram extraídos para obtenção de cortes histológicos, com o objetivo de se avaliar o grau de formação de ponte dentinária. Os dados obtidos foram submetidos à análise estatística (Kruskal-Wallis, p<0,05). De acordo com a ação antimicrobiana, os materiais foram classificados na seguinte ordem decrescente: hidróxido de cálcio P.A., pasta A, pasta C, pasta B, óleo de Pterodon emarginatus Vogel e óleo de Copaifera langsdorffii Desfon, com diferenças estatisticamente significantes entre o hidróxido de cálcio P.A., as pastas e os veículos (p≤0,05). Ao final do tempo experimental, observou-se que as três pastas avaliadas foram semelhantes quanto à formação de ponte dentinária. Considerando-se os microrganismos selecionados para o trabalho, conclui-se que as pastas avaliadas foram semelhantes entre si em termos de potencial antimicrobiano, diferindo dos componentes individuais (p≤0,05). Em relação à formação de ponte dentinária, ao final do tempo experimental, não foram observadas diferenças significantes entre os dentes tratados com as diferentes pastas.Universidade de FrancaBrasilPós-GraduaçãoPrograma de Mestrado em Cirurgia e Anestesiologia VeterináriaUNIFRANHonsho, Cristiane dos Santos9135785602712466http://lattes.cnpq.br/9135785602712466Casemiro, Luciana Assirati9439742609603039http://lattes.cnpq.br/9439742609603039Teófilo, Juliana MazzonetoSouza, Fabiana Ferreira de7294582289403049http://lattes.cnpq.br/7294582289403049Dias, Fernanda Gosuen Gonçalves2020-05-22T19:03:26Z2020-05-22T19:03:26Z2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfDIAS, Fernanda Gosuen Gonçalves. Pastas endodônticas formuladas com óleos vegetais: ação em microbiota bucal e na formação de ponte dentinária em cães. Franca, 2011. 67 f. Dissertação (Mestrado em Cirurgia e Anestesiologia Veterinária) - Universidade de Franca. 2011.https://repositorio.cruzeirodosul.edu.br/handle/123456789/695porinfo:eu-repo/semantics/openAccessreponame:Repositório do Centro Universitário Braz Cubasinstname:Centro Universitário Braz Cubas (CUB)instacron:CUB2020-05-22T19:05:28Zoai:repositorio.cruzeirodosul.edu.br:123456789/695Repositório InstitucionalPUBhttps://repositorio.brazcubas.edu.br/oai/requestbibli@brazcubas.edu.bropendoar:2020-05-22T19:05:28Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB)false
dc.title.none.fl_str_mv Pastas endodônticas formuladas com óleos vegetais: ação na microbiota bucal e na formação de ponte dentinária em cães
Endodontics pastes formulated with vegetable oils: action on oral microbiota and on dentin bridge formation in dogs
title Pastas endodônticas formuladas com óleos vegetais: ação na microbiota bucal e na formação de ponte dentinária em cães
spellingShingle Pastas endodônticas formuladas com óleos vegetais: ação na microbiota bucal e na formação de ponte dentinária em cães
Dias, Fernanda Gosuen Gonçalves
Endodontia - Cães
Microbiologia
Capeamento da polpa dentária
Plantas medicinais
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA
title_short Pastas endodônticas formuladas com óleos vegetais: ação na microbiota bucal e na formação de ponte dentinária em cães
title_full Pastas endodônticas formuladas com óleos vegetais: ação na microbiota bucal e na formação de ponte dentinária em cães
title_fullStr Pastas endodônticas formuladas com óleos vegetais: ação na microbiota bucal e na formação de ponte dentinária em cães
title_full_unstemmed Pastas endodônticas formuladas com óleos vegetais: ação na microbiota bucal e na formação de ponte dentinária em cães
title_sort Pastas endodônticas formuladas com óleos vegetais: ação na microbiota bucal e na formação de ponte dentinária em cães
author Dias, Fernanda Gosuen Gonçalves
author_facet Dias, Fernanda Gosuen Gonçalves
author_role author
dc.contributor.none.fl_str_mv Honsho, Cristiane dos Santos
9135785602712466
http://lattes.cnpq.br/9135785602712466
Casemiro, Luciana Assirati
9439742609603039
http://lattes.cnpq.br/9439742609603039
Teófilo, Juliana Mazzoneto
Souza, Fabiana Ferreira de
7294582289403049
http://lattes.cnpq.br/7294582289403049
dc.contributor.author.fl_str_mv Dias, Fernanda Gosuen Gonçalves
dc.subject.por.fl_str_mv Endodontia - Cães
Microbiologia
Capeamento da polpa dentária
Plantas medicinais
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA
topic Endodontia - Cães
Microbiologia
Capeamento da polpa dentária
Plantas medicinais
CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA
description This study evaluated the action of endodontics pastes formulated with calcium hydroxide P.A. and different vehicles (physiological solution – paste A, Pterodon emarginatus Vogel oil - paste B and Copaifera langsdorffii Desfon oil - paste C) against oral microorganisms, and on bridge dentin formation in dogs. The antimicrobial action of the pastes and their individual components were evaluated by the technique of determining the minimum inhibitory concentration (MIC) by agar dilution. The materials were diluted at concentrations of 0,5%, 1,0%, 2,0% and 4,0%, and evaluated against fifteen standard strains of aerobic, microaerophilic, anaerobic and facultative anaerobes microorganisms that are related to endodontic diseases. The microrganisms were grown in ideal conditions and, after incubation, the data were obtained and analyzed (Anova One-Way, Tukey, p≤0,05). For the assessment of dentin bridge formation, six upper incisors of ten animals were treated, being the right side and the middle capped with paste A (Group A), the central right and left with paste B (Group B) and the medial and left lateral side teeth with paste C (Group C). After 90 days, the teeth were extracted to obtain histological sections, with the objective of evaluating the degree of dentin bridge formation. The data were subjected to statistical analysis (Kruskal-Wallis, p≤0,05). According to the antimicrobial action, the materials were classified in the following decrescent order: calcium hydroxide P.A., paste A, paste C, paste B, Pterodon emarginatus Vogel oil, and Copaifera langsdorffii Desfon oil, with statistically significant differences between the hydroxide calcium P.A., pastes, and vehicle (p ≤ 0,05). At the end of experimental time, it was observed that the three pastes were similar in relation to the formation of dentin bridge. Considering the microorganisms selected for this study, it was concluded that the pastes were similar in terms of antimicrobial potential, differing from the individual components (p≤0,05). Regarding to the formation of dentin bridge, at the end of experimental period, there were no significant differences between the teeth treated with different pastes.
publishDate 2011
dc.date.none.fl_str_mv 2011
2020-05-22T19:03:26Z
2020-05-22T19:03:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv DIAS, Fernanda Gosuen Gonçalves. Pastas endodônticas formuladas com óleos vegetais: ação em microbiota bucal e na formação de ponte dentinária em cães. Franca, 2011. 67 f. Dissertação (Mestrado em Cirurgia e Anestesiologia Veterinária) - Universidade de Franca. 2011.
https://repositorio.cruzeirodosul.edu.br/handle/123456789/695
identifier_str_mv DIAS, Fernanda Gosuen Gonçalves. Pastas endodônticas formuladas com óleos vegetais: ação em microbiota bucal e na formação de ponte dentinária em cães. Franca, 2011. 67 f. Dissertação (Mestrado em Cirurgia e Anestesiologia Veterinária) - Universidade de Franca. 2011.
url https://repositorio.cruzeirodosul.edu.br/handle/123456789/695
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de Franca
Brasil
Pós-Graduação
Programa de Mestrado em Cirurgia e Anestesiologia Veterinária
UNIFRAN
publisher.none.fl_str_mv Universidade de Franca
Brasil
Pós-Graduação
Programa de Mestrado em Cirurgia e Anestesiologia Veterinária
UNIFRAN
dc.source.none.fl_str_mv reponame:Repositório do Centro Universitário Braz Cubas
instname:Centro Universitário Braz Cubas (CUB)
instacron:CUB
instname_str Centro Universitário Braz Cubas (CUB)
instacron_str CUB
institution CUB
reponame_str Repositório do Centro Universitário Braz Cubas
collection Repositório do Centro Universitário Braz Cubas
repository.name.fl_str_mv Repositório do Centro Universitário Braz Cubas - Centro Universitário Braz Cubas (CUB)
repository.mail.fl_str_mv bibli@brazcubas.edu.br
_version_ 1798311343082700800